Comparison of fast versus slow rewarming following acute moderate hypothermia in rats

The aim of this study was to determine the effects of erythropoietin (EPO), moderate hypothermia, and a combination thereof on the kidneys of newborn rats damaged during perinatal asphyxia. An animal model of perinatal asphyxia (Wistar rats) was used in which after birth, newborn rats were divided into four groups of 15 animals each: G1, rats exposed only to asphyxia; G2, rats exposed to asphyxia and hypothermia (rectal temperature 32°C) and which received EPO (darbepoetin alpha) intraperitoneally; G3, rats exposed to asphyxia and hypothermia; G4, rats exposed to asphyxia and which received EPO. The rats were sacrificed on the 7th day of life and histopathological evaluation of kidneys was performed. Damage to the proximal tubules was significantly higher in group G1 rats than in groups G2, G3, and G4 rats (p < 0.01). Damage to the distal tubules was found only in group G1 rats. Histological changes in the proximal tubules were more prominent than in the distal tubules (p < 0.01). The immature glomeruli zone was less expressed in group G4 rats than in groups G1, G2, and G3 rats (p < 0.01). Based on these results, we conclude that EPO and hypothermia, as well as the combination thereof, have a protective effect on rats' kidneys damaged during perinatal asphyxia.

show abstract

“…Clinical studies investigating the therapeutic use of hypothermia recommend a warming rate no faster than 0.5°C/h [28].…”

Section: Discussionmentioning

confidence: 99%

The influence of EPO and hypothermia on the kidneys of rats after perinatal asphyxia

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Although optimal methods for re-warming were not tested in newborn animals, adult animal studies indicated that slow re-warming was preferred. 15,16 …”

Section: Pathophysiologic Basis For Therapeutic Strategiesmentioning

confidence: 99%

Hypothermia and Other Treatment Options for Neonatal Encephalopathy: An Executive Summary of the Eunice Kennedy Shriver NICHD Workshop

Higgins¹,

Raju²,

Edwards³

et al. 2011

The Journal of Pediatrics

201

138

View full text Add to dashboard Cite

“…Evaluations of animal models including fetal sheep, newborn mice, rat pups, piglets, and nonhuman primates2—7, 9, 12, 13, 15, 16, 23–24 with timed hypoxic-ischemic injury have been performed and been reviewed 25, 26…”

Section: Pathogenesis Of Hiementioning

confidence: 99%

Hypothermia for hypoxic ischemic encephalopathy in infants ≥36weeks

Higgins

Shankaran

2009

Early Human Development

View full text Add to dashboard Cite

Hypoxic ischemic encephalopathy is a serious condition affecting infants which can result in death and disability. This is a summary of pathogenesis of HIE, animal studies of cooling for hypoxic and ischemic models, human hypothermia trials, and the American Academy of Pediatrics publication on hypothermia for HIE. Hypothermia for neonatal HIE is continuing to evolve as a therapy. Studies, gaps in knowledge and opportunities for research are presented herein.

show abstract

Comparison of fast versus slow rewarming following acute moderate hypothermia in rats

Cited by 16 publications

References 34 publications

The influence of EPO and hypothermia on the kidneys of rats after perinatal asphyxia

The influence of EPO and hypothermia on the kidneys of rats after perinatal asphyxia

Hypothermia and Other Treatment Options for Neonatal Encephalopathy: An Executive Summary of the Eunice Kennedy Shriver NICHD Workshop

Hypothermia for hypoxic ischemic encephalopathy in infants ≥36weeks

Contact Info

Product

Resources

About