Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus

Abstract

The present observation records the lipid classes and their fatty acid composition of the neutral lipid and phospholipid of the trematode Paramphistomum cervi and liver of its host Capra hircus, the common Indian goat. Thin Layer Chromatography and Gas Liquid Chromatography were used to identify different neutral lipid and phospholipid components. The results show that among the neutral lipid fractions, the amount of combined hydrocarbon, wax ester and steryl ester is more or less equal in parasite and its host, but the percent of triacylglycerol is more in host liver than the parasite and the percent of total sterol is more in parasite than that of its host liver. Among the phospholipid fractions, phosphatidylinositol and phosphatidylethanolamine in the trematode parasite P. cervi and phosphatidylcholine and cardiolipin in the host's liver are the major components. The predominant fatty acids in the neutral lipid and phospholipid fractions of both the host liver and the trematode P. cervi include C16:0, C18:0, C18:1, and C18:2. The results reveal that the parasite P. cervi take up almost all the lipid and fatty acids from their host, which is required for their life cycle.

Variation in lipid and fatty acid uptake among nematode and cestode parasites and their host, domestic fowl: host–parasite interaction

“…Cestode also absorbs more C 18:1 from WE. Palmitoleic (C 16:1 ) is also found which was also reported by Ghosh and Misra (2014) in WE of P. cervi.…”

“…All the samples have considerable amount of dienoic fatty acids among PL fractions. Linoleic (C 18:2 ) acid is predominant among all the samples which was also reported by Aisien and Ogiji (1989) and Ghosh and Misra (2014). F. hepatica can form C 20:2 from C 18:2 (Barrett 1981) and S. mansoni can elongate linoleic series of fatty acids to C 22:4 (Fripp et al 1976).…”

“…Liver has high level of SE (7.53 %), nematode shows 0.96 %, whereas, cestode have 1.96 %. According to Ghosh and Misra (2014), SE is low in P. cervi than the host. Paratenuisentis ambiguus has specific mechanisms for the regulation of the sterol and fatty acid composition of its NLs and cholesterol as the major sterol in both P. ambiguous and its host Anguilla anguilla (Weber et al 1994).…”

“…Therefore, it can be assumed that stigmasterols play an important role in the life cycles of both the parasites i.e. The result showed that R. (F.) echinobothrida is rich (53 %) in cholesterol; P. cervi (Ghosh and Misra 2014) absorbs greater quantity of campesterol (47 %) and stigmasterol (49 %) than other, whereas A. galli takes all the three sterols in moderate amount; Raillietina and Paramphistomum take up minimum of stigmasterol and cholesterol, respectively. These sterols might be related to their greater reproductive efficiency.…”

“…The total analysis of major lipid classes and fatty acids composition has already been done on trematode parasite, Paramphistomum cervi, rumen fluid and liver of the mammalian host, Capra hircus (Ghosh and Misra 2011). Ghosh and Misra (2014) made did further analysis by comparing the fatty acid components of the NLs and PLs of the trematode, P. cervi and liver of its mammalian host, C. hircus. Similar studies have not been performed in an avian system.…”