Comparison of fertile and sterile male gametogenesis in Cryptomeria japonica D. Don

Futamura, Norihiro; Igasaki, Tomohiro; Saito, Maki; Taira, Hideaki; Shinohara, Kenji

doi:10.1007/s11295-019-1335-8

“…Out of these genes, MS1 is the most frequent in C. japonica male-sterility breeding materials, with 11 male-sterile trees homozygous for MS1 ( ms1/ms1 ) and five male-fertile trees heterozygous for MS1 ( Ms1/ms1 ) found in Japan 5 , 6 , 11 – 18 . The C. japonica trees that are homozygous for ms1 become male-sterile due to the failure of exine development during microspore formation 16 , 19 , but it remains unclear whether MS1 is caused by a single genetic mutation event. Additionally, there is no information about the geographical distribution of the genetic mutation responsible for MS1 .…”

Section: Introductionmentioning

confidence: 99%

Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)

Hasegawa

¹

,

Wei

²

,

Matsumoto

³

et al. 2021

Sci Rep

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Identifying causative genes for a target trait in conifer reproduction is challenging for species lacking whole-genome sequences. In this study, we searched for the male-sterility gene (MS1) in Cryptomeria japonica, aiming to promote marker-assisted selection (MAS) of male-sterile C. japonica to reduce the pollinosis caused by pollen dispersal from artificial C. japonica forests in Japan. We searched for mRNA sequences expressed in male strobili and found the gene CJt020762, coding for a lipid transfer protein containing a 4-bp deletion specific to male-sterile individuals. We also found a 30-bp deletion by sequencing the entire gene of another individual with the ms1. All nine breeding materials with the allele ms1 had either a 4-bp or 30-bp deletion in gene CJt020762, both of which are expected to result in faulty gene transcription and function. Furthermore, the 30-bp deletion was detected from three of five individuals in the Ishinomaki natural forest. From our findings, CJt020762 was considered to be the causative gene of MS1. Thus, by performing MAS using two deletion mutations as a DNA marker, it will be possible to find novel breeding materials of C. japonica with the allele ms1 adapted to the unique environment of each region of the Japanese archipelago.

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“…breeding materials, with 11 male-sterile trees homozygous for MS1 (ms1/ms1) and five male-fertile trees heterozygous for MS1 (Ms1/ms1) found in Japan 5,6,[11][12][13][14][15][16][17][18] . The C. japonica trees that are homozygous for ms1 become male-sterile due to the failure of exine development during microspore formation 16,19 , but it remains unclear whether MS1 is caused by a single genetic mutation event. Additionally, there is no information about the geographical distribution of the genetic mutation responsible for MS1.…”

mentioning

confidence: 99%

Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonicaD. Don)

Hasegawa

¹

,

Ueno

²

,

Wei

³

et al. 2020

Preprint

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Identifying causative genes for a target trait in conifer reproduction is challenging for species lacking whole-genome sequences. In this study, we searched for the male-sterility gene (MS1) in Cryptomeria japonica, aiming to promote marker-assisted selection (MAS) of male-sterile C. japonica to reduce the pollinosis caused by pollen dispersal from artificial C. japonica forests in Japan. We searched for mRNA sequences expressed in male strobili and found the gene CJt020762, coding for a lipid transfer protein containing a 4-bp deletion specific to male-sterile individuals. We also found a 30-bp deletion by sequencing the entire gene of another individual with the ms1. All nine breeding materials with the allele ms1 had either a 4-bp or 30-bp deletion in gene CJt020762, both of which are expected to result in faulty gene transcription and function. Furthermore, the 30-bp deletion was detected from three of five individuals in the Ishinomaki natural forest. From our findings, CJt020762 was considered to be the causative gene of MS1. Thus, by performing MAS using two deletion mutations as a DNA marker, it will be possible to find novel breeding materials of C. japonica with the allele ms1 adapted to the unique environment of each region of the Japanese archipelago.

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“…According to Tsubomura et al (2016), who categorized the gene expression of C. japonica male strobili into eight clusters along the developmental stage, the expression pattern of CJt020762 may be classified into C7 (genes expressed in the early and middle developmental stages). Unfortunately, the past gene expression studies by microarray [7,63] were unable to find this candidate gene, as these microarrays did not have probes designed for the gene.…”

Section: Expression Differences Between Male-fertile and -Sterile Sammentioning

confidence: 99%

Construction of a reference transcriptome for the analysis of male sterility in sugi (Cryptomeria japonica D. Don) focusing on MALE STERILITY 1 (MS1)

Wei

¹

,

Ueno

²

,

Ujino‐Ihara

³

et al. 2021

Self Cite

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Sugi (Cryptomeria japonica D. Don) is an important conifer used for afforestation in Japan. As the genome of this species is 11 Gbps, it is too large to assemble within a short timeframe. Transcriptomics is one approach that can address this deficiency. Here we designed a workflow consisting of three stages to de novo assemble transcriptome using Oases and Trinity. The three transcriptomic stage used were independent assembly, automatic and semi-manual integration, and refinement by filtering out potential contamination. We identified a set of 49,795 cDNA and an equal number of translated proteins. According to the benchmark set by BUSCO, 87.01% of cDNAs identified were complete genes, and 78.47% were complete and single-copy genes. Compared to other full-length cDNA resources collected by Sanger and PacBio sequencers, the extent of the coverage in our dataset was the highest, indicating that these data can be safely used for further studies. When two tissue-specific libraries were compared, there were significant expression differences between male strobili and leaf and bark sets. Moreover, subtle expression difference between male-fertile and sterile libraries were detected. Orthologous genes from other model plants and conifer species were identified. We demonstrated that our transcriptome assembly output (CJ3006NRE) can serve as a reference transcriptome for future functional genomics and evolutionary biology studies.

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Comparison of fertile and sterile male gametogenesis in Cryptomeria japonica D. Don

Cited by 8 publications

References 79 publications

Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)

Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)

Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonicaD. Don)

Construction of a reference transcriptome for the analysis of male sterility in sugi (Cryptomeria japonica D. Don) focusing on MALE STERILITY 1 (MS1)

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