Background: Various connections have been machined to improve the fit between the dental abutment and implant. In vivo, the instability created by imprecisely fitting components can cause soft tissue irritation and bacterial colonization of the implant system. The aim of this study was to quantify abutment stability under in vitro force applications. Methods: Abutment stability and fit were quantitatively measured after application of rotational, vertical, and horizontal forces. Findings: The abutment connection held by friction (Friction-Fit) was the only group to have 0°angular rotation. A significantly greater vertical force was required to pull the abutment from the implant for the Friction-Fit connection as compared to all other experimental groups. The abutment connection held by a mechanically locking friction-fit with four grooves (CrossFit) and Friction-Fit demonstrated significantly lower lateral movement as compared to all other connections. The remaining connections evaluated included two hexagon connections that rely on screw placement for abutment fit (Conical + Hex #1 and Conical + Hex #2), one connection with protruding slots to align with recessed channels inside the implant (Conical + 6 Indexing Slots), and an internal connection that allows for abutment indexing every 120°(Internal Tri-Channel). Interpretation: Internal connection geometry influenced the degree of abutment movement. Friction-Fit and CrossFit connections exhibited the lowest rotational and horizontal motions. Significant differences were found between Friction-Fit and CrossFit following the application of a vertical force, with the Friction-Fit requiring a significantly greater pull force to separate the abutment from the implant.