Comparison of Four Different Isolation Media for Staphylococcus aureus

오민희,; 강성일,; 홍상필,; 오세욱,

doi:10.3746/jkfn.2009.38.5.606

Journal of the Korean Society of Food Science and Nutrition

2009

DOI: 10.3746/jkfn.2009.38.5.606

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Comparison of Four Different Isolation Media for Staphylococcus aureus

오민희 오민희¹,

강성일 강성일²,

홍상필 홍상필³

et al.

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Cited by 2 publications

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Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products

Kim¹,

Lee²,

Seo³

et al. 2012

Journal of Food Hygiene and Safety

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-Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), BairdParker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat (60 o C for 90s), and cold (−20 o C for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.

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Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products

Kim¹,

Lee²,

Seo³

et al. 2012

Journal of Food Hygiene and Safety

View full text Add to dashboard Cite

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Toxin Genes and Antibiotic Resistance of Food Poisoning Bacteria Isolated from Food Service Equipment in Childcare Centers

Kim,

et al. 2024

J. Fd Hyg. Safety

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Comparison of Four Different Isolation Media for Staphylococcus aureus

Cited by 2 publications

References 10 publications

Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products

Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products

Toxin Genes and Antibiotic Resistance of Food Poisoning Bacteria Isolated from Food Service Equipment in Childcare Centers

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