1994
DOI: 10.1128/jcm.32.7.1816-1819.1994
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of four different methods for epidemiologic typing of Acinetobacter baumannii

Abstract: A set of 103 epidemiologically well-defined Acinetobacter baumannii isolates obtained from nine hospital outbreaks and 21 unrelated strains were characterized by pulsed-field gel electrophoresis (PFGE) of total genomic DNA digested with ApaI. Among outbreak strains, eight different patterns and five possible variants were identified by PFGE. Results were compared with those from traditional typing methods such as plasmid profile analysis, antimicrobial susceptibility, and biotyping. Plasmid analysis revealed s… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
41
0
3

Year Published

1995
1995
2015
2015

Publication Types

Select...
4
2
2

Relationship

1
7

Authors

Journals

citations
Cited by 87 publications
(44 citation statements)
references
References 27 publications
0
41
0
3
Order By: Relevance
“…In addition, A. baumannii blood culture isolates recovered from patients in 49 medical institutions of various sizes throughout the USA between 1995 and 1998 (SCOPE Project [24]) were analyzed. Epidemiological details of the 74 isolates investigated have been described previously [6,15,25] and are summarized in Table 1. Identification of Acinetobacter spp.…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…In addition, A. baumannii blood culture isolates recovered from patients in 49 medical institutions of various sizes throughout the USA between 1995 and 1998 (SCOPE Project [24]) were analyzed. Epidemiological details of the 74 isolates investigated have been described previously [6,15,25] and are summarized in Table 1. Identification of Acinetobacter spp.…”
Section: Methodsmentioning
confidence: 99%
“…The relatedness of all isolates was assessed using RAPD‐PCR analysis with two different primers (ERIC‐2 and M13) and Ready‐to‐Go RAPD Analysis beads (Pharmacia Biotech, Freiburg, Germany) as described previously [16]. Analysis was confirmed using PFGE analysis of genomic DNA digested with Apa I as described previously [6]. Fingerprint patterns were compared visually and by Molecular Analyst software (Bio‐Rad Laboratories, Munich, Germany).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Three of these are common in clinical culture material, namely, A. baumannii (DNA group 2), the unnamed DNA group 3, and Tjernberg and Ursing's DNA group 13 (29). A. baumannii seems to be the most prevalent Acinetobacter species isolated from clinical specimens in hospitals (6,22), and most hospital outbreaks have been attributed to this species (15,16,25,27). However, in these reports the strains have been identified phenotypically only and therefore the identification may not be fully reliable.…”
mentioning
confidence: 99%
“…These genes were not detected in any isolate. Pulsed-field gel electrophoresis of SmaI-digested genomic DNA was performed with a CHEF-DRIII system (Bio-Rad, Hemel Hempstead, England) (13). The technique was applied to the 35 isolates, and banding patterns were compared visually by following the criteria of Tenover et al (16).…”
mentioning
confidence: 99%