Comparison of four different sampling methods for detecting pharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae in children

Rapola, Satu; Salo, Eeva; Kiiski, Pirjo; Leinonen, Maija; Takala, Aino K.

doi:10.1128/jcm.35.5.1077-1079.1997

Cited by 84 publications

(34 citation statements)

References 14 publications

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“…In general, the detection rates differ for each respiratory virus but are especially high in RSV-infected patients [24]. The detection rates for common (Streptococcus pneumoniae and Haemophilus influenzae) and atypical bacterial pathogens (M. pneumoniae and C. pneumoniae) are comparable between NPA and nasal swab [25,26].…”

Section: Discussionmentioning

confidence: 99%

High antibiotic prescription rates in hospitalized children with human metapneumovirus infection in comparison to RSV infection emphasize the value of point-of-care diagnostics

et al. 2018

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hMPV infections inherit a two times higher probability of antibiotic treatment. There was no significant difference in laboratory findings or body temperature between hMPV infection and infections caused by other pathogens. Clinical symptoms seem not to differ from those in RSV illness. Nonetheless, RSV infections triggered significantly lower antibiotic prescription rates. A considerate application of a POC-mPCR for patients with RSV-like symptoms and age of 1 year and older with a negative RSV-RADT might lead to higher detection rates of hMPV and a reduction in prescription of antibiotics.

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Section: Discussionmentioning

confidence: 99%

High antibiotic prescription rates in hospitalized children with human metapneumovirus infection in comparison to RSV infection emphasize the value of point-of-care diagnostics

et al. 2018

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“…Nevertheless, studies comparing two sampling techniques suggest that the yield of both methods were similar for H. influenzae . 22,23 Crowding or number of siblings in a family was reported to increase Hib carriage rate. 19,24,25 The influence of concurrent upper respiratory symptoms on Hib carriage rates remains controversial, and studies differ in their findings on the influence of season, sex, tobacco exposure and race on carriage rates.…”

Section: Discussionmentioning

confidence: 99%

Pharyngeal colonization with Haemophilus influenzae type b among healthy Turkish infants and children

Bakır

Yağcı²,

Ülger³

et al. 2002

Pediatrics International

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Medicine, Pittsburgh, PA I n c o n t r a s t t o normotensive adults, b o r d e r l i n e hypertensives S t r a t e e i e s f o r management o f children attending DC f a c i l i t i e s do n o t demonstrate forearm v a s o d i l a t i o n w i t h a h i a h sodium d i e t a f t e r occErrence of a Ease of HIB disease a r e controversial. ?he success o f chemoprophylaxis has been v a r i a b l e. Failure of rifam-p i n prophylaxis a s c u r r e n t l y recommended, may r e s u l t from usage limited t o d i r e c t contacts o f t h e index case. ?his prospective study was designed t o a s c e r t a i n the e x t e n t of colonization i n household contacts o f colonized children attending DC f a c i l i t i e s with an index case of HIB disease. Outer membrane p r o t e i n (OMP) analysis was used t o determine s i m i l a r i t y between s t r a i n s i s o l a t-ed from contacts and index cases. In DC children from 6 f a c i l i t-i e s , 15% were colonized with i d e n t i c a l subtypes of HIB. In add-i t i o n , 7% o f children i n the l a r g e r DC centers c a r r i e d non-iden-t i c a l subtypes. Colonization with i d e n t i c a l subtypes i n children from DC homes was g r e a t e r than i n t h e l a r g e r DC centers (91% vs 8%, p<0.00001). Within families o f children with i d e n t i c a l OMP subtypes, 25% (17% of parents and 44% o f s i b l i n g s) were colonized despite lack of d i r e c t contact with t h e index case. ?his colon-i z a t i o n r a t e was comparable t o t h a t of household contacts o f index cases. Of household contacts o f DC children with non-ident-i c a l subtypes of HIB, 13% were colonized. W e conclude t h a t colonized household contacts a r e a p o t e n t i a l source of HIB i n f e c t i o n f o r susceptible D C children and may a l s o warrant prophylaxis. and show augmented nevrogenic v a s o c o n s t r i c t o r responses d u r i n g b a r o r e f l e x s t i m u l a t i o n. Children from t h e lower (LoQ) and upper (HiQ) q u i n t i l e s o f blood pressure (BP) d i s t r i b u t i o n were examined. Six adolescent males from each group were studied on each o f three d i e t s : ad l i b , low sodium (10 mEq) and h i g h sodium (310 mEq). U r i n a r y sodium, aldosterone and k a l l i k r e i n and plasma r e n i n confirmed d i e t a r y compl iance and sodium balance. Weight, BP and h e a r t r a t e (HR) d i d n o t change. During h i g h sodium, f o r e-arm vascular r e s i s t a n c e (FVR) decreased from 16.2 t o 10.4 U (p< 0.05) i n LoQ. However, HiQ d i d n o t show a decrease i n FVR, 16.3 vs 15.6 U (p>0.05). Both groups responded s i m i l a r l y t o c a r d i o-pulmonary b a r o r e f l e x i n h i b i t i o n induced by lower body negative pressure w i t h s i g n i f i c a n t l y (p<0.01) increased HR, decreased forearm blood f l o w and increased FVR. HR response was augmented d u r i n g low sodium (p<0.001). P o s i t i v e and negative pressure applied t o t h e neck a f f e c t e d t h e h i g h pressure b a r o r e f l e x c o n t r o l ...

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“…Although obtaining a nasopharyngeal (NP) swab is minimally invasive, performance of multiple tests usually requires obtaining multiple specimens. Although some investigators have reported using nasal samples for multiple tests, (1)(2)(3)(4)(5) we are not aware of any studies that have used a single NP swab to evaluate viral, bacterial and host parameters.…”

Section: Introductionmentioning

confidence: 99%

A method of processing nasopharyngeal swabs to enable multiple testing

et al. 2019

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Objective To develop a method to perform multiple tests on single nasopharyngeal (NP) swab. Study design We collected a NP swab on children aged 2–12 years with acute sinusitis and processed it for bacterial culture, viruses, cytokine expression, and 16S ribosomal RNA gene sequencing analysis. During the course of the study, we expand the scope of evaluation to include RNA sequencing, which we accomplished by cutting the tip of the swab. Results Of the 174 children enrolled, 126 (72.4%) had a positive bacterial culture and 121(69.5%) tested positive for a virus. Cytokine measurement, as judged by the adequate levels of a housekeeping enzyme (GAPDH), appeared successful. From the samples used for 16S ribosomal sequencing we recovered, on average, 16,000 sequences per sample, accounting for a total of 2,646 operational taxonomic units across all samples sequenced. Samples used for RNA sequencing had a mean RNA Integrity number of 6.0. Cutting the tip of the swab did not affect the recovery yield for viruses or bacteria, nor did it affect species richness in microbiome analysis. Conclusion We describe a minimally invasive sample collection protocol that allows for multiple diagnostic and research investigations in young children.

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Comparison of four different sampling methods for detecting pharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae in children

Cited by 84 publications

References 14 publications

High antibiotic prescription rates in hospitalized children with human metapneumovirus infection in comparison to RSV infection emphasize the value of point-of-care diagnostics

High antibiotic prescription rates in hospitalized children with human metapneumovirus infection in comparison to RSV infection emphasize the value of point-of-care diagnostics

Pharyngeal colonization with Haemophilus influenzae type b among healthy Turkish infants and children

A method of processing nasopharyngeal swabs to enable multiple testing

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