Comparison of four hematology analyzers, CELL‐DYN Sapphire, ADVIA 120, Coulter LH 750, and Sysmex XE‐2100, in terms of clinical usefulness

Abstract

Summary We evaluated the clinical usefulness (leukocyte distribution classification, morphologic classification, and morphologic flags) of the following four hematology analyzers: CELL‐DYN Sapphire (CD‐Sapphire) (Abbott Diagnostics, Santa Clara, CA, USA), ADVIA 120 (Bayer Diagnostics, Tarrytown, NY, USA), Beckman Coulter LH 750 (Beckman Coulter, Miami, FL, USA), and Sysmex XE‐2100 (TOA Medical Electronics Co., Kobe, Japan). Four hundred thirty samples from patients and 100 samples from healthy individuals were analyzed. For distributional classification, the sensitivity rates of CD‐Sapphire, ADVIA 120, LH 750, and XE‐2100 were 93.1, 95.9, 94.9, and 94.9%, respectively, and the efficiency rates were 80.7, 81.6, 84.1, and 84.2%, respectively. For morphologic classification, the sensitivity rates of CD‐Sapphire, ADVIA 120, LH 750, and XE‐2100 were 88.6, 93.2, 77.3, and 94.3%, respectively, and the efficiency rates were 80.9, 73.0, 79.5, and 74.2%, respectively. Comparing the findings in different morphologic flags, XE‐2100 showed the highest sensitivity for Blasts flag (90.9%); CD‐Sapphire showed the highest sensitivity for Immature granulocytes and/or Left‐shift flag (85.5%); ADVIA 120 showed the highest sensitivity for Atypical lymphocytes flag (60.0%); and LH 750 showed the highest sensitivity for Nucleated RBC flag (75.0%). Our results demonstrate that the four analyzers are comparable in overall performance.

Evaluation of ABX Pentra DX 120 and Sysmex XE‐2100 in umbilical cord blood

“…The high sensitivity of Sysmex XE‐2100 blast flag has been reported in comparison with the other analyzers. Such a finding has been related to the false‐positive results . Our observation is in line with the previous studies, which were performed on adults' peripheral blood.…”

Acute leukemia detection rate by automated blood count parameters and peripheral smear review

“…Even extrapolating our results to those using ADVIA analyzers is problematic because of blast flag interlaboratory variation. In our study, the frequencies of blast flags in those without acute leukemia in both settings were low, whereas others have reported the frequency of blast flags to be 14.2% and 51% in such patients, most likely due to analyzer variation. Furthermore, our study did not examined the influence of the smear review findings on the identification of diseases other than acute leukemia.…”

Biomarkers of Hypochromia: The Contemporary Assessment of Iron Status and Erythropoiesis

“…It has been published the new extended RBC parameters as measured on CELL-DYN Sapphire show a high degree of correlation with those of the Advia analyzers, although the absolute values may differ [42, 43]. This renders it necessary to establish instrument-specific reference ranges and clinical decision values [44, 45].…”