Comparison of four kits for the detection of staphylococcal enterotoxin in foods from outbreaks of food poisoning

Wieneke, Antonnette A.

doi:10.1016/0168-1605(91)90122-6

Cited by 51 publications

(29 citation statements)

References 5 publications

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“…Ingestion results in nausea, vomiting and diarrhoea while inhalation provokes non-productive cough, chest pain and dyspnea. Classical ways of detection are mainly based on multiplex PCR (McLauchlin et al 2000;Mehrotra et al 2000) and immunoassays (Freed et al 1982;Wieneke 1991), which allow quantitative or qualitative measurements in less than 24 hours. A peptide displaying-phage for the optical detection of SEB with biosensor technology was developed by Goldman et al (2000).…”

Section: Detection Of Staphylococcal Enterotoxin B (Seb)mentioning

confidence: 99%

The Use of Phages and Aptamers as Alternatives to Antibodies in Medical and Food Diagnostics

Mehta¹,

Dorst²,

Devriese³

et al. 2011

Biomedical Engineering, Trends, Research and Technologies

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Section: Detection Of Staphylococcal Enterotoxin B (Seb)mentioning

confidence: 99%

The Use of Phages and Aptamers as Alternatives to Antibodies in Medical and Food Diagnostics

Mehta¹,

Dorst²,

Devriese³

et al. 2011

Biomedical Engineering, Trends, Research and Technologies

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“…The Bommeli SET-EIA test is a solid-phase ELISA that uses polystyrene beads coated with antibodies against staphylococcal enterotoxins A-D. Wienke (1991) compared the Bommeli kit with three other commercial kits and found it was the most sensitive for the detection of staphylococcal enterotoxin in foods from food poisoning outbreaks. The kit detected enterotoxin in 14 of 18 foods, compared with nine for RPLA test and 10 for Transia ELISA.…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

“…Rose et al (1989) found that non-specific reactions could be reduced by the addition of 10 nmol l −1 hexametaphosphate to the diluent. Wienke (1991) compared four immunological test kits for the detection of staphylococcal enterotoxin in food and concluded that no single method was superior, although the SET-RPLA was the cheapest and easiest to perform.…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

A review of analytical methods for the detection of bacterial toxins

Pimbley

Patel

1998

Journal of Applied Microbiology

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“…SET RPLA could differentiate the enterotoxins A-D but its use is time-consuming. Due to the use of latex particles, no interference with endogenous enzymes has been observed [18]. The introduction of molecular techniques and the availability of DNA sequence information for all described SEs have allowed the detection of specific enterotoxin gene sequences by Polymerase Chain Reaction (PCR) [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Enterotoxigenic coagulase positive Staphylococcus in milk and milk products, lben and jben, in northern Morocco

Bendahou

Abid

Bouteldoun

et al. 2009

J Infect Dev Ctries

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Background: The aim of this research was to determine the prevalence of enterotoxin genes (sea-seo) in Coagulase Positive Staphylococcus (CPS) isolated from unpasteurized milk and milk products. These results were compared with the results obtained by using the detection kit SET-RPLA for the specific detection of staphylococcal enterotoxins (SEA-SED). Methodology: Eighty-one samples of milk and milk products were analyzed for the presence of Staphylococcus strains. Forty-six coagulase positive Staphylococcus isolates were tested for the production of staphylococcal enterotoxins (SEA-SED) by using the reversed passive latex agglutination method. The strains were also tested for the presence of se genes (sea-seo) by polymerase chain reaction. Results: One or more classical enterotoxin products (SEA-SED) were observed in 39% of the strains tested, while se genes were detected in 56.5%. SEA and sea were most commonly detected. For newly discovered se genes among CPS isolates tested in this study, except the seh gene which was revealed in four isolates (8.7 %), none of the strains harbored any of the other se genes (see, seg, sei, sej, sek, sel, sem, seo and sen). Conclusions: The finding of a pathogen such as staphylococci-producing SEs and containing se genes in milk and milk products in northern Morocco may indicate a problem for public health in this region. The presence of enterotoxigenic strains in food does not always necessarily mean that the toxin will be produced. For that reason, the combination of both methods (RPLA and PCR) is a guarantee for success in diagnostic analysis tests.

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Comparison of four kits for the detection of staphylococcal enterotoxin in foods from outbreaks of food poisoning

Cited by 51 publications

References 5 publications

The Use of Phages and Aptamers as Alternatives to Antibodies in Medical and Food Diagnostics

The Use of Phages and Aptamers as Alternatives to Antibodies in Medical and Food Diagnostics

A review of analytical methods for the detection of bacterial toxins

Enterotoxigenic coagulase positive Staphylococcus in milk and milk products, lben and jben, in northern Morocco

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