Comparison of four mechanical methods to retrieve spermatozoa from testicular tissue

Verheyen, Greta; Croo, Ilse De; Tournaye, Herman; Pletincx, I.; Devroey, Paul; Steirteghem, A.C. Van

doi:10.1093/oxfordjournals.humrep.a135828

Cited by 123 publications

(47 citation statements)

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“…The aim of tissue processing is to facilitate sperm search, and both mechanical and enzymatic techniques yield similar results (28,29). After processing, epididymal and testicular spermatozoa can be either used for ICSI or cryopreserved.…”

Section: Laboratory Processing Of Surgi-cally-retrieved Spermmentioning

confidence: 99%

Sperm retrieval techniques for assisted reproduction

Esteves¹,

Miyaoka²,

Agarwal³

2011

Int. braz j urol.

127

117

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Different surgical methods such as PESA, MESA, TESA, TESE and micro-TESE have been developed to retrieve spermatozoa from either the epididymis or the testis according to the type of azoospermia, i.e., obstructive or non-obstructive. Laboratory techniques are used to remove contaminants, cellular debris, and red blood cells following collection of the epididymal fluid or testicular tissue. Surgically-retrieved spermatozoa may be used for intracytoplasmic sperm injection (ICSI) and/or cryopreservation. In this article, we review the surgical procedures for retrieving spermatozoa from both the epididymis and the testicle and provide technical details of the commonly used methods. A critical analysis of the advantages and limitations of the current surgical methods to retrieve sperm from males with obstructive and non-obstructive azoospermia is presented along with an overview of the laboratory techniques routinely used to process surgically-retrieved sperm. Lastly, we summarize the results from the current literature of sperm retrieval, as well as the clinical outcome of ICSI in the clinical scenario of obstructive and nonobstructive azoospermia.

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Section: Laboratory Processing Of Surgi-cally-retrieved Spermmentioning

confidence: 99%

Sperm retrieval techniques for assisted reproduction

Esteves¹,

Miyaoka²,

Agarwal³

2011

Int. braz j urol.

127

117

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“…A few groups have reported some elimination of RBC with lysate buffer (6,28). However, in our experience, the removal of RBC from TESE specimens with lysate buffer is only minimal.…”

Section: Discussionmentioning

confidence: 63%

Development of a new and efficient laboratory method for processing testicular sperm.

Hammitt¹,

Sattler²,

Ferrigni³

et al. 2001

Fertility and Sterility

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Purpose : Testicular biopsy specimens contain large amounts of debris that makes sperm pickup for ICSI more difficult than with epididymal aspirates. We sought to develop improved processing techniques for testicular sperm extraction (TESE). Methods : Retrievals were with azoospermic male partner scheduled to undergo percutaneous epididymal sperm aspiration (PESA) and TESE. The study group consisted of 9 retrievals with a new TESE technique (TESE-N). The control group was 21 retrievals with PESA and 3 retrievals with a previous TESE technique (TESE-P). Results : TESE-N eliminated almost all debris, which made ICSI sperm pick-up more rapid. TESE-N, PESA, and TESE-P fertilization (77, 75, and 72%) and ongoing/delivered pregnancy rates per retrieval (67, 76, and 67%) were similar. Conclusions : Our new technique provides for easy removal of debris from TESE specimens and fertilization and pregnancy rates equal to epididymal sperm's. Eliminating debris from TESE specimens allows for rapid sperm pick-up for ICSI, making the procedure more efficient for embryology staff.

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“…Six of them had spermatozoa in their testicular tissue (positive) and the remaining five had none (negative). The mean age was 33.2 years (range [27][28][29][30][31][32][33][34][35][36][37][38] for the positive group and 33.0 years (range 27-38) for the negative group. All 11 men had been diagnosed as being azoospermic after at least two consecutive semen analyses, with an abnormal 47,XXY constitutional blood karyotype.…”

Section: Infertile Patientsmentioning

confidence: 99%

“…Initial microscopic examination of the wet preparation was performed at ×400 magnification under an inverted microscope with the Hoffman Modulation Contrast System (Modulation Optics Inc., Greenvale, NY, USA). The testicular tissue was centrifuged at 300×g for 7 min and the pellet was suspended with 2 ml of erythrocyte-lysing buffer [27] for 10 min. The small testicular tissue specimen underwent enzymatic treatment with 1, 000 IU/ml collagenase type IV (Sigma-Aldrich, C5138) in order to completely digest the testicular tissue [28].…”

Section: Testicular Cell Retrievalmentioning

confidence: 99%

Ploidy of spermatogenic cells of men with non-mosaic Klinefelter’s syndrome as measured by a computerized cell scanning system

Komsky-Elbaz

Raziel

Ben‐Ami

et al. 2015

J Assist Reprod Genet

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Purpose This study aims to characterize the origin of testicular post-meiotic cells in non-mosaic Klinefelter's syndrome (KS). Methods The study included testicular tissue specimens from 11 non-mosaic KS patients, with (6 positive) and without (5 negative) spermatozoa presence. The obtained testicular cells were affixed and stained for morphology followed by fluorescence in situ hybridization (FISH) for centromeric probes X, Y, and 18. We used a computerized automated cell scanning system that enables simultaneous viewing of morphology and FISH in the same cell. Results A total of 12,387 cells from the positive cases, 11,991 cells from the negative cases, and 1,711 cells from the controls were analyzed. The majority of spermatogonia were 47, XXY in both the positive and negative KS cases (88.9±4.76 % and 90.6±4.58 %) as were primary spermatocytes (76.8±8.14 % and 79.6±7.30 %). The respective rates of secondary spermatocytes and post-meiotic cells (round, elongating spermatids and sperm cells) were 1.1±1.39 % in the positive cases, 2.9±3.33 % in the negative cases, compared to 67.6±6.22 % in the controls (P<0.02). Pairing of both 18 and XY homologous chromosomes in 46,XY primary spermatocytes was 2.5 ±2.31 % and 3.4±2.39 %, respectively, compared to 19.8± 8.95 % in the control group (P<0.02) and in 47,XXY primary spermatocytes in 2.4±3.8 % in the positive group and 3.2± 2.26 % in the negative group. Conclusions This study presents data to indicate that the majority of primary spermatocytes in the testes of non-mosaic KS patients are 47,XXY and could possibly develop into postmeiotic cells.

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Comparison of four mechanical methods to retrieve spermatozoa from testicular tissue

Cited by 123 publications

References 0 publications

Sperm retrieval techniques for assisted reproduction

Sperm retrieval techniques for assisted reproduction

Development of a new and efficient laboratory method for processing testicular sperm.

Ploidy of spermatogenic cells of men with non-mosaic Klinefelter’s syndrome as measured by a computerized cell scanning system

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