Comparison of four monoclonal antibodies reacting with gastric gland mucous cell-derived mucins of rat and frog

Kurihara, Makoto; Ishihara, Kazuhíko; Ota, Hiroyoshi; Katsuyama, Tsutomu; Nakano, T.; Naito, Manami; Hotta, Kyoko

doi:10.1016/s0305-0491(98)10113-x

Cited by 9 publications

(2 citation statements)

References 15 publications

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“…To clarify this, we carried out HIK1083 immunohistochemical staining. The epitope that reacts with HIK1083 is a mucinous carbohydrate chain whose non-reduced end is connected to N -acetylglucosamine in the α form ( 24 ) ; this epitope is common to different species of vertebrates ( 25 ) . In the present study, most of the secreted mucous was removed in the process of preparing the specimen for analysis; thus, the part of the tissue that was stained with the HIK1083 antibody indicated gland mucous cells.…”

Section: Discussionmentioning

confidence: 99%

Viscous methyl cellulose solution thickens gastric mucosa and increases the number of gland mucous cells in mice

Takabayashi

Sekiguchi

2013

Br J Nutr

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We have observed a stiffening of the gastric wall in mice following oral administration of viscous methyl cellulose (MC) solution as a vehicle for food chemicals, an effect which has not previously been reported. To further investigate this effect, young male ICR mice (n 48) were divided into four groups (one control and three experimental groups) of twelve mice each. Experimental groups I, II and III were administered 0·2 ml of 0·5 % (w/v) MC viscous solution/d via a stomach tube for 2, 3 and 4 weeks, respectively. Stomachs were collected following 12 h fasting. The weight of the stomach and mucosa of seven mice per group was measured, and the mucosa was used for the measurement of 8-oxo-2 0 -deoxyguanosine (8-oxodG). Stomach tissue slices from the remaining five mice per group were stained with haematoxylin and eosin to measure mucosal thickness, and were immunostained with an HIK1083 antibody to determine the number of gastric gland mucous cells. The mucosal thickness of the groups administered the MC solution for 3 and 4 weeks, and the size of the HIK1083-immunostained area of the groups administered the MC solution for 2, 3 and 4 weeks were significantly increased (P,0·01) compared with those of the control group. The amount of 8-oxodG was not significantly affected. These results indicate that oral administration of viscous MC solution leads to the thickening of the gastric mucosa, which may be related to an increase in the number of gland mucous cells.

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Section: Discussionmentioning

confidence: 99%

Viscous methyl cellulose solution thickens gastric mucosa and increases the number of gland mucous cells in mice

Takabayashi

Sekiguchi

2013

Br J Nutr

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“…Briefly, COS-1 cells, which are originally negative for αGlcNAc, were co-transfected with a human stomach cDNA library constructed in the mammalian expression vector pcDNAI together with a cDNA encoding the membrane-bound sialoglycoprotein of leukocytes leukosialin (CD43) which contains 80 O -glycans in its extracellular domain [3]. Transfected cells were then screened using monoclonal antibodies specific for αGlcNAc, including HIK1083 [8], PGM36, and PGM37 [14]. Transfected cells recognized by any of these antibodies were enriched by fluorescence-activated cell sorting.…”

Section: Molecular Cloning and Expression Of α4gnt The Enzyme Responmentioning

confidence: 99%

Dual Roles of Gastric Gland Mucin-specific <i>O</i>-glycans in Prevention of Gastric Cancer

Nakayama

2014

Acta Histochem. Cytochem

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Gastric gland mucin is secreted from gland mucous cells, including pyloric gland cells and mucous neck cells located in the lower layer of the gastric mucosa. These mucins typically contain O-glycans carrying terminal α1,4-linked N-acetylglucosamine residues (αGlcNAc) attached to the scaffold protein MUC6, and biosynthesis of the O-glycans is catalyzed by the glycosyltransferase, α1,4-N-acetylglucosaminyltransferase (α4GnT). We previously used expression cloning to isolate cDNA encoding α4GnT, and then demonstrated that αGlcNAc functions as natural antibiotic against Helicobacter pylori, a microbe causing various gastric diseases including gastric cancer. More recently, it was shown that αGlcNAc serves as a tumor suppressor for differentiated-type adenocarcinoma. This review summarizes these findings and identifies dual roles for αGlcNAc in gastric cancer.

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Alpha-1,4-N-Acetylglucosaminyltransferase (A4GNT)

Nakayama

2014

Handbook of Glycosyltransferases and Related Genes

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Comparison of four monoclonal antibodies reacting with gastric gland mucous cell-derived mucins of rat and frog

Cited by 9 publications

References 15 publications

Viscous methyl cellulose solution thickens gastric mucosa and increases the number of gland mucous cells in mice

Viscous methyl cellulose solution thickens gastric mucosa and increases the number of gland mucous cells in mice

Dual Roles of Gastric Gland Mucin-specific <i>O</i>-glycans in Prevention of Gastric Cancer

Alpha-1,4-N-Acetylglucosaminyltransferase (A4GNT)

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