Comparison of Four Parasitological Techniques for Laboratory Diagnosis of Eggs from Spirometra spp. in Wild Mammal Fecal Samples

Dib, Laís Verdan; Palmer, João Pedro Siqueira; Lima, Camila de Souza Carvalho Class de; Ramos, Raissa Cristina Ferreira; Bastos, Otílio Machado Pereira; Uchôa, Cláudia Maria Antunes; Amendoeira, Maria Regina Reis; Fonseca, Ana Beatriz Monteiro; Bastos, Augusto Cezar Machado Pereira; Barbosa, Alynne da Silva

doi:10.2478/s11686-019-00120-1

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…Diagnoses of parasites of the family Diphyllobothriidae were only obtained using the spontaneous sedimentation technique, suggesting greater sensitivity for the diagnosis of cestodes of this family compared to other techniques. This corroborates the data reported by Dib et al (2019) , who compared different coproparasitological techniques in the diagnosis of S. mansonoides (Pseudophyllidea: Diphyllobothriidae) in wild mammals and observed greater sensitivity in sedimentation techniques than in flotation techniques.…”

Section: Discussionsupporting

confidence: 91%

“…The diagnosis of the trematode P. illiciens indicated a possible difference between the techniques, scoring better performance of the CFS and spontaneous sedimentation techniques when compared to that of Faust et al (1939) . A positive performance of the sedimentation technique was expected because it is indicated for the diagnosis of heavy eggs such as trematodes ( Foreyt, 1989 ; Dib et al, 2019 ). However, the CFS technique, which is routinely used for the diagnosis of oocysts and helminth eggs, demonstrated excellent performance in the diagnosis of P. illiciens .…”

Section: Discussionmentioning

confidence: 99%

“…This result is similar to that found by Rocha et al (2014) , who compared the CFS technique with centrifugal sedimentation in formalin-ether (the standard technique in the diagnosis of P. illiciens ) and observed greater performance in the diagnosis of P. illiciens by CFS. Dib et al (2019) explained that this occurred because the high density of sucrose solution allowed some sealed eggs to float.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of gastrointestinal parasites in domestic cats (Felis catus) diagnosed by different coproparasitological techniques in the municipality of Seropédica, Rio de Janeiro

Silva,

Campos,

Lima

et al. 2023

Rev. Bras. Parasitol. Vet.

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The objectives of this work were to investigate the occurrence of parasites in feces of cats, compare different coproparasitological techniques for their diagnosis and determine associations with parasitism. The samples were processed using three different coproparasitological techniques: centrifugal flotation in sucrose, centrifugal flotation in ZnSO4 and simple sedimentation. The parasitic association between parasitism and variables such as age, sex and fecal consistency was performed using the chi-square test or the G test with a significance level of 5%. A total of 237 samples were analyzed, of which 93 (39.2%) were positive, being Ancylostoma spp. (17.3%), Giardia intestinalis (12.2%), Platynosomum illiciens (8.0%), Cystoisospora spp. (6.3%), Toxoplasma gondii/ Hammondia hammondi (3.4%), Diphyllobothriidae (2.1%), Toxocara spp. (1.7%), Dipylidium caninum (1.3%) and Mesocestoides spp. (0.8%). In the parasitism association analysis, it was possible to verify a statistical difference in the age category for Cystoisospora spp. (p=0.001) observing a strong relationship between parasitism and young animals, the association with sex proved to be important for P. illiciens (p<0.001) with a higher frequency of parasitized females and fecal consistency revealed to be related to the parasites G. intestinalis (p=0.007) and P. illiciens (p=0.033) showing a higher number of positive animals for these parasites with normal fecal consistency. In conclusion, we observed a higher occurrence of Ancylostoma spp. and G. intestinalis in fecal samples from domestic cats received in routine diagnoses and the presence of other parasites with zoonotic potential, as well as the relationship of these diagnosed parasites with the categories sex, age and fecal consistency.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Prevalence of gastrointestinal parasites in domestic cats (Felis catus) diagnosed by different coproparasitological techniques in the municipality of Seropédica, Rio de Janeiro

Silva,

Campos,

Lima

et al. 2023

Rev. Bras. Parasitol. Vet.

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“…The different performance observed in the recovery of B. coli cysts from fecal samples from pigs could be associated with the consistency of the sample and the parasitic infection intensity. Moreover, the amount of fecal material examined and the sugar-salt solution used in the coprological analysis [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison between five coprological methods for the diagnosis of Balantidium coli cysts in fecal samples from pigs

Pinilla

Florez

2021

Vet World

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Background and Aim: Balantidium coli is a protozoan that can infect humans and non-human primates, being the domestic pigs the animals most affected by this parasite. This study aimed to compare the performance of five coprological methods for diagnosis of Balantidium coli cysts in fecal samples from pigs. Materials and Methods: From September to December 2019, 558 fecal samples were collected from the rectum of backyard pigs in the Bucaramanga metropolitan area, Colombia. The average age of the sampled animals was 3-4 months. Fecal samples collected were tested using the following coprological techniques: Direct examination with Lugol's iodine solution, buffered saline, centrifugation/flotation and McMaster techniques, and Ziehl–Neelsen method. Results: The results indicate that B. coli cysts were diagnosed most frequently through direct examination with Lugol's iodine solution (52.7% of the samples) followed by direct examination with a buffered saline solution (37.6%). Moderate concordance (k=0.41; p<0.05) was determined between direct method with Lugol's iodine and buffered saline solution, McMaster and buffered saline (k=0.35; p<0.05), and centrifugation/flotation and buffered saline (k=0.28; p<0.05) showed a fair degree of concordance. The rest of the comparisons were classified as poor. The flotation techniques (centrifugation/ flotation and McMaster) did not show good recovery of cysts. Conclusion: It is concluded that the most efficient method for diagnosing this parasitosis was to an association between direct examination with Lugol's iodine solution and buffered saline.

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“…Nowadays, I also work on the assessment of parasite biodiversity in captive and free living wild animals, both in urban and wild environments, including protected areas in Atlantic Forest biome such as Serra dos Órgãos National Park, Itatiaia National Park and PedraSelada State Park, and in their surroundings. [ 1 2 3 4 5 ] In addition, our research groups have been working with protozoa that can cause damage to the health of animals and even problems to public health due to the potential for zoonotic transmission evidenced by any of these agents. Within this context, helminths and apicomplexan protozoa are included, which can infect domestic animals in urban and rural environments.…”

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confidence: 99%

Face to face

Barbosa

2021

Tropical Parasitology

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Comparison of Four Parasitological Techniques for Laboratory Diagnosis of Eggs from Spirometra spp. in Wild Mammal Fecal Samples

Cited by 6 publications

References 19 publications

Prevalence of gastrointestinal parasites in domestic cats (Felis catus) diagnosed by different coproparasitological techniques in the municipality of Seropédica, Rio de Janeiro

Prevalence of gastrointestinal parasites in domestic cats (Felis catus) diagnosed by different coproparasitological techniques in the municipality of Seropédica, Rio de Janeiro

Comparison between five coprological methods for the diagnosis of Balantidium coli cysts in fecal samples from pigs

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