Comparison of four tests to evaluate the reactivity of rabbit sera against envelope or Gag-related proteins of bovine leukemia virus (BLV)

Production and Characterization of Monoclonal Antibodies against Bovine Leukaemia Virus using Various Crude Antigen Preparations: a Comparative Study

“…The second criterion was that the purity of the immunogen for the production of mAbs is irrelevant, as seen by a number of authors ( Galfre and Milstein, 1981; Zola and Brooks, 1982). In addition, we also used PV ( Buck et al, 1988), owing not only to the simplicity of the preparation, but also to its immunogenicity ( Domenech et al, 1998). This semi‐purification protocol had not been used before for the production of mAbs.…”

“…Polyclonal serum antibodies were not able to react in WB with gp51SU that had undergone protein denaturation ( Domenech et al, 1998). Other immunological tests, such as AGID or inhibition of syncytia formation, must be used to confirm anti‐gp51SU antibodies presence ( Domenech et al, 1998).…”

“…Polyclonal serum antibodies were not able to react in WB with gp51SU that had undergone protein denaturation ( Domenech et al, 1998). Other immunological tests, such as AGID or inhibition of syncytia formation, must be used to confirm anti‐gp51SU antibodies presence ( Domenech et al, 1998). Nevertheless, some of our mAbs produced by hyperimmunizing mice with antigen preparations where gp51SU may have been presented in a different protein conformation from the native protein, can detect gp51SU in the conformation that is encountered in WB as previously described above.…”

Analysis by Sodium Dodecyl Sulfate Polyacrylamide gel Electrophoresis and Western Blot of Nonspecific and Specific Viral Proteins Frequently Detected in Different Antigen Preparations of Bovine Leukemia Virus

“…The difficulty of detecting antibodies against gp51 using WB was previously described 11 ; hence, a consensus among the different tests should be used to confirm whether an animal is infected with BLV. 11 The 2 BLV-encoded regulatory proteins, Tax (34-38 kD 25 ) and Rex (16-19 kD 24 ), that are present in BLV-expressing cells but not in virions were not observed. Both antibodies against Tax and Rex have been detected in serum from cattle and sheep in the late tumor stage.…”

“…14,28 However, WB results, although helpful and very informative, are difficult to interpret if adequate controls are not taken into consideration. 11 A number of cell lines have been developed by various investigators. FLK-BLV derived from fetal lamb kidney 27 and BLV-bat 2 from mature bat lung 13 have been the cell lines of choice for investigators studying BLV.…”