2010
DOI: 10.1111/j.1742-4658.2009.07490.x
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Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis

Abstract: Antifreeze proteins are structurally diverse polypeptides that have thermal hysteresis activity and have been discovered in many cold-adapted organisms. Of these, fungal antifreeze protein has been purified and partially characterized only in a species of psychrophilic basidiomycete, Typhula ishikariensis. Here we report a new fungal antifreeze protein from another psychrophile, Antarctomyces psychrotrophicus. We examined its biochemical properties and thermal hysteresis activity, and compared them with those … Show more

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Cited by 86 publications
(53 citation statements)
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“…X-ray diffraction data were collected to 0.95-Å resolution at −173°C on BL44B2 at the SPring-8. The electron density map showed one-to-one correspondence with the amino acid sequence of TisAFP6, thereby showing that only TisAFP6 had only been crystallized out of the the mixture of isoforms (25). The crystallographic R factor and free R factor of the final structure were 0.112 and 0.129, respectively.…”
Section: Methodsmentioning
confidence: 91%
See 1 more Smart Citation
“…X-ray diffraction data were collected to 0.95-Å resolution at −173°C on BL44B2 at the SPring-8. The electron density map showed one-to-one correspondence with the amino acid sequence of TisAFP6, thereby showing that only TisAFP6 had only been crystallized out of the the mixture of isoforms (25). The crystallographic R factor and free R factor of the final structure were 0.112 and 0.129, respectively.…”
Section: Methodsmentioning
confidence: 91%
“…The snow mold fungus, Typhula ishikariensis, attaches onto dormant plants and secretes its AFP (TisAFP) toward the extracellular space under snow cover (25). Purified TisAFP is a mixture of 223-residue polypeptide isoforms.…”
mentioning
confidence: 99%
“…AFPs are extracellular polypeptides that depress the freezing point of water and modify the structure of ice crystals. They have been described in cold-adapted organisms, among which the basidiomycetes Coprinus psychromorbidus (Hoshino et al, 2003a) and Typhula ishikariensis (Hoshino et al, 2003b) and the ascomycete Antarctomyces psychrotrophicus (Xiao et al, 2010). Blast against Aspergillus genomes did not give any hit.…”
Section: Mechanisms Of Freeze Tolerance and Possible Role Of Dprcmentioning
confidence: 96%
“…Only two mushrooms (enoki and shiitake), one snow mold fungus ( Typhula ishikariensis ), and two yeast organisms ( Glaciozyma antarctica and Glaciozyma sp. AY30) have been characterized both genomically and for their antifreeze properties [9,10,140,141]. …”
Section: Marine-derived Afpsmentioning
confidence: 99%