Comparison of Genome Sequence of PVY Isolates with Biological Properties

Abstract

The whole RNA genomes of six European Potato virus Y (PVY) isolates, representing isolates of the PVY O , PVY N and PVY NTN subgroups, were sequenced and the reactions of a range of tobacco and potato cultivars after the inoculation were determined. One isolate induced symptoms of veinal necrosis when inoculated to tobacco, but reacted with PVY O -specific antibodies. The genome sequence of this isolate showed that it had a recombinant genome comprising large sections of sequence that are characteristic of N and O sub-group isolates. Another isolate caused mosaic symptoms when inoculated to tobacco, and reacted with PVY N -specific antibodies. The nucleotide sequence of its genome was very closely related to the genome sequence published for two potato-infecting PVY isolates from North America. These three isolates are the members of interesting group NA (North American). They have highly similar genome sequences throughout their length, but biologically represent isolates from the PVY O , PVY N and PVY NTN subgroups. The occurrence of these biologically diverse isolates with their highly homologous genomes brings into question the exact nature of gene structure in relation to function.Resumen Se secuenciaron los genomas completos de ARN de seis aislamientos europeos del virus Y de la papa (PVY) representantes de aislamientos de los subgrupos de PVY O , PVY N y PVY NTN y se determinaron las reacciones de una serie de cultivares de tabaco y papa después de las inoculaciones. Un aislamiento indujo síntomas de necrosis de las nervaduras cuando fue inoculado en tabaco pero reaccionó con los anticuerpos específicos de PVY O . La secuencia del genoma de este aislamiento mostró que tenía un genoma recombinante con grandes secciones de secuencias que son características de aislamientos de los subgrupos N y O. Otro aislamiento causó síntomas de mosaico cuando se inoculó a tabaco y reaccionó con anticuerpos específicos de PVY N . La secuencia de nucleótidos de su genoma fue relacionada muy cercanamente con la secuencia publicada de dos aislamientos de PVY de Norteamérica que infectan papa. Estos tres aislamientos son miembros del grupo NA (Norte Americano). Ellos tienen una secuencia de genoma altamente similar, pero biológicamente representan aislamientos de los subgrupos PVY O , PVY N y PVY NTN . La presencia de estos aislamientos biológica-mente diferentes con sus genomas altamente homólogos, pone en cuestión la naturaleza exacta del gen con relación a su función.

Recombinants of PVY Strains Predominate Among Isolates from Potato Crop in Poland

“…Overall, our data confirms the large diversity of PVY species that exists, even within the isolates collected from potato, and is similar to the diversity reported by others (Piche et al 2004;Baldauf et al 2006;Barker et al 2009;Nie et al 2011). The occurrence of biologically diverse isolates with their highly homologous genomes brings into question the exact nature of gene structure in relation to function (Barker et al 2009). Point mutation has been shown to be responsible for serotype variation of certain isolates (Chikh Ali et al 2007;Karasev et al 2010).…”

Potato virus Y (PVY) in Seed Potato Certification

“…2011). However, Barker et al (2009) who studied NTN isolates in greenhouse tests described virus symptoms as severe, systemic necrosis and early death of plant up to no visible symptoms despite high absorbance values in ELISA depending on potato cultivars. In another study, Physalis floridana as the test plant in greenhouse tests was infected by two different NTN isolates of the same strain.…”

Resistance to multiple viruses in transgenic tobacco expressing fused, tandem repeat, virus-derived double-stranded RNAs

“…The cDNA clones were of PVA (strain U [17]), PVY (strain O, SCRI-O isolate [18]), PLRV (Canadian isolate [19]), PMTV (Swedish isolate [20]), and TRV (isolate PpK20 [21] The 600-bp and a 1000-bp fused, tandem viral sequences were prepared using overlapping PCR (Supplementary Fig. S1) with the primer pairs listed in Supplementary Table S1.…”