Comparison of Ginsenoside Contents and Pattern Similarity Between Root Parts of New Cultivars in Panax ginseng C.A. Meyer

안인옥,; 이성식,; 이장호,; 이미자,; 조병구,

doi:10.5142/jgr.2008.32.1.015

Cited by 30 publications

(8 citation statements)

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“…Ginsenoside profiles and accumulation patterns can differ based on genotype (Ahn et al, 2008; Kim et al, 2009) and growth conditions (Matkowski, 2008). In this study, we focused on the genetic component of ginsenoside accumulation by using five genetically fixed inbred cultivars, CP, CS, GO, SH, and SU (Kwon et al, 2001; Ahn et al, 2008; Lee et al, 2008) and isolating ginsenosides from in vitro cultured adventitious roots grown under a controlled environment.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we focused on the genetic component of ginsenoside accumulation by using five genetically fixed inbred cultivars, CP, CS, GO, SH, and SU (Kwon et al, 2001; Ahn et al, 2008; Lee et al, 2008) and isolating ginsenosides from in vitro cultured adventitious roots grown under a controlled environment. We quantified the variation among cultivars in the contents of ginsenosides and intermediates in the ginsenoside biosynthesis pathway as well as the relevant differences in expression of the underlying genes.…”

Section: Discussionmentioning

confidence: 99%

“…Dozens of ginseng accessions have been bred through pure line selection from three local landrace populations (Jakyung, Chungkyung, and Hwangsook) and registered as cultivated varieties (cultivars) in Korea (Kim et al, 2012). These cultivars exhibit different morphological and physiological characters (Kim et al, 2012; Lee et al, 2015), as well as different metabolite accumulation patterns (Ahn et al, 2008; Kim et al, 2009; Lee et al, 2011; Cho et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

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Integrated Transcriptomic and Metabolomic Analysis of Five Panax ginseng Cultivars Reveals the Dynamics of Ginsenoside Biosynthesis

et al. 2017

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Panax ginseng C.A. Meyer is a traditional medicinal herb that produces bioactive compounds such as ginsenosides. Here, we investigated the diversity of ginsenosides and related genes among five genetically fixed inbred ginseng cultivars (Chunpoong [CP], Cheongsun [CS], Gopoong [GO], Sunhyang [SH], and Sunun [SU]). To focus on the genetic diversity related to ginsenoside biosynthesis, we utilized in vitro cultured adventitious roots from the five cultivars grown under controlled environmental conditions. PCA loading plots based on secondary metabolite composition classified the five cultivars into three groups. We selected three cultivars (CS, SH, and SU) to represent the three groups and conducted further transcriptome and gas chromatographymass spectrometry analyses to identify genes and intermediates corresponding to the variation in ginsenosides among cultivars. We quantified ginsenoside contents from the three cultivars. SH had more than 12 times the total ginsenoside content of CS, with especially large differences in the levels of panaxadiol-type ginsenosides. The expression levels of genes encoding squalene epoxidase (SQE) and dammarenediol synthase (DDS) were also significantly lower in CS than SH and SU, which is consistent with the low levels of ginsenoside produced in this cultivar. Methyl jasmonate (MeJA) treatment increased the levels of panaxadiol-type ginsenosides up to 4-, 13-, and 31-fold in SH, SU, and CS, respectively. MeJA treatment also greatly increased the quantity of major intermediates and the expression of the underlying genes in the ginsenoside biosynthesis pathway; these intermediates included squalene, 2,3-oxidosqualene, and dammarenediol II, especially in CS, which had the lowest ginsenoside content under normal culture conditions. We conclude that SQE and DDS are the most important genetic factors for ginsenoside biosynthesis with diversity among ginseng cultivars.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrated Transcriptomic and Metabolomic Analysis of Five Panax ginseng Cultivars Reveals the Dynamics of Ginsenoside Biosynthesis

et al. 2017

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“…In the present study, to homogenize the edaphic and environmental conditions, different wild-collected licorice genotypes were first cultivated in the same location and then investigated for the desired phytochemical and morphological traits. Some reports have indicated the use of selective breeding to introduce superior varieties for Ephedra sinica 32 and Panax ginseng 33 .…”

Section: Discussionmentioning

confidence: 99%

Introducing the glycyrrhizic acid and glabridin rich genotypes from the cultivated Iranian licorice (Glycyrrhiza glabra L.) populations to exploit in production systems

Esmaeili,

Mirjalili,

Karami

et al. 2024

Sci Rep

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Currently, the stable, uniform, and highly efficient production of raw materials for pharmaceutical companies has received special attention. To meet these criteria and reduce harvesting pressure on the natural habitats of licorice (Glycyrrhiza glabra L.), cultivation of this valuable plant is inevitable. In the present study, to introduce the glycyrrhizic acid (GA)- and glabridin-rich genotypes from cultivated Iranian licorice, forty genotypes from eight high-potential wild populations were cultivated and evaluated under the same environmental conditions. The GA content varied from 5.00 ± 0.04 mg/g DW (TF2 genotype) to 23.13 ± 0.02 mg/g DW (I5 genotype). The highest and lowest glabridin content were found in the K2 (0.72 ± 0.021 mg/g DW) and M5 (0.02 ± 0.002 mg/g DW) genotypes, respectively. The rutin content in the leaves of the studied genotypes varied from 1.27 ± 0.02 mg/g DW in E4 to 3.24 ± 0.02 mg/g DW in BO5 genotypes. The genotypes from the Ilam population were characterized by higher vegetative growth and yield traits in the aerial parts and roots. The average root dry yield was 2.44 tons per hectare (t/ha) among the studied genotypes and a genotype from Ilam (I5) yielded the maximum value (3.08 ± 0.034 t/ha). The highest coefficient of variation among the genotypes was observed for leaf width (CV = 34.9%). The GA and glabridin-rich genotypes introduced in this study can be used in the future breeding programs to release new bred licorice cultivars.

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“…However, previous studies on ginseng are limited to investigations such as ginsenoside content analysis (Samukawa et al, 1995; Shi et al, 2013), quality standardization methods (Ahn et al, 2008), and analysis of bioactivity by variety or country of origin (Dong et al, 2011; Zhang et al, 2014). Therefore, in this study we designed a three-stage systematic study to explore the physiological activity of ginseng.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Ginsenoside Profiles: Antioxidant, Antiproliferative, and Antigenotoxic Activities of Ginseng Extracts of Fine and Main Roots

Seo

Choi

Kim

et al. 2019

pnf

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The aim of this study was to compare ginsenosides profiles, and antioxidant, antiproliferative, and antigenotoxic activities of ginseng extract derived from fine and main roots. The result of the analysis showed a higher total content of ginsenoside in fine roots than in main roots; differences in levels between the different extracts were also confirmed. The oxygen radical absorbance capacity (ORAC) assay showed that H2O main root extract had a significantly higher activity than that from fine roots. MeOH and H2O extracts from the fine and main roots also exhibited stronger cellular antioxidant capacity 2,2’-azobis(2-amidinopropane) dihydrochloride-induced oxidative stress in HepG2 cells compared with the positive control. Through calculating the half-maximal inhibitory concentration values, the cytotoxicity of the main root extracts were ranked as follows: MeOH (6.1±1.2 μg/mL)> H2O (6.6±0.1 μg/mL)> ethanol (10.4±0.6 μg/mL); however, the cytotoxicity of all fine root extracts did not significantly differ. All the fine root extracts showed an inhibitory capacity against 4-hydroxynonenal-induced DNA damage, however only the MeOH extract of the main root showed a decrease in DNA damage. All three solvent extracts from the fine roots reduced DNA damage more in the H2O2-treated group, whereas only the MeOH and H2O extracts of the main roots produced a significant reduction. Levels of Rg3 ginsenoside were positively correlated with indices of the ORAC value, and total ginsenoside contents showed a negative correlation with DNA damage induced by H2O2. This study suggests that ginseng and the extraction solvent both affect levels of ginsenoside. Furthermore, the antioxidant potency of ginseng can be attributed to the content of some ginsenosides.

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Comparison of Ginsenoside Contents and Pattern Similarity Between Root Parts of New Cultivars in Panax ginseng C.A. Meyer

Cited by 30 publications

References 1 publication

Integrated Transcriptomic and Metabolomic Analysis of Five Panax ginseng Cultivars Reveals the Dynamics of Ginsenoside Biosynthesis

Integrated Transcriptomic and Metabolomic Analysis of Five Panax ginseng Cultivars Reveals the Dynamics of Ginsenoside Biosynthesis

Introducing the glycyrrhizic acid and glabridin rich genotypes from the cultivated Iranian licorice (Glycyrrhiza glabra L.) populations to exploit in production systems

Comparative Analysis of Ginsenoside Profiles: Antioxidant, Antiproliferative, and Antigenotoxic Activities of Ginseng Extracts of Fine and Main Roots

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