Comparison of GN Hajna and Tetrathionate as Initial Enrichment for Salmonellae Recovery from Swine Lymph Nodes and Cecal Contents Collected at Slaughter

Harvey, Roger B.; Anderson, Robin C.; Farrington, Leigh A.; Droleskey, Robert E.; Genovese, Kenneth J.; Ziprin, Richard L.; Nisbet, David J.

doi:10.1177/104063870101300314

Cited by 8 publications

(8 citation statements)

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“…In addition, satisfactory isolation of Salmonella Choleraesuis using RV broth and no inhibitory effect by TT broth for this serotype have been reported. 4 As noted by others 3,6 and on the basis of our experience in previous investigations, results reported here corroborate with the observation that method A is significantly (P Ͻ 0.05) less efficient than method B, indicating that it is not a good choice for food safety studies.…”

Section: Culture Methods Differ On the Isolation Of Salmonella Enterica Serotypes From Naturally Contaminated Swine Fecal Samplessupporting

confidence: 92%

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

et al. 2005

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Abstract. Four culture methods (A, B, C, and D) were comparatively evaluated for their ability to isolate Salmonella enterica from pooled swine fecal samples (n ϭ 100). None of the methods was able to isolate Salmonella from all positive samples. The relative sensitivity of the culture methods evaluated was 82%, 94%, 95%, and 78% for methods A, B, C, and D, respectively. The comparison of sensitivities showed that methods B and C performed significantly better (P Ͻ 0.05) than methods A and D. Although relative sensitivities of methods B and C were equal, from the 89 positive samples concomitantly detected by both, 35 (39.3%) had different serotypes (no match) isolated by each method. On the basis of the results of this study, it was concluded that culture methods differ on the isolation of S. enterica serotypes from naturally contaminated swine fecal samples. Depending on the objective(s) of investigations on the ecology and epidemiology of S. enterica in swine populations, a method or a combination of methods should be considered for more reliable results.An incredible number of different culture methods for isolation of Salmonella have been published, but currently, there appears to be no consensus on what would be the best method. 12 As a consequence, confusing and contradictory results are frequently found in the literature.For years, 2 culture methods (methods A and B, described below) have been concomitantly applied in our laboratory routine for isolation of Salmonella from swine samples. 13 A recent study 3 compared these methods with another frequently applied culture method (preenrichment in buffered peptone water, followed by enrichment in Rappaport-Vassiliadis [RV] broth and isolation on Xylose-Lysine-Tergitol-4 [XLT-4] agar), reporting better results when isolation was conducted using method B. However, we felt that continuous im- Disclaimer: Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement of the U.S. Department of Agriculture. provement in the sensitivity of culture methods was necessary and should be persistently pursued. Therefore, culture methods C and D (described below) were developed and included in this study, whose objective was to comparatively evaluate 4 different culture methods (methods A, B, C, and D) and to assess their relative sensitivity for the isolation of Salmonella enterica from naturally contaminated swine fecal samples. The current knowledge of the effect of different culture methods on the isolation of different S. enterica serotypes from naturally contaminated samples is very limited. Most studies published to date have simply focused on the sensitivity of culture methods for the detection of positive samples presenting little information on the isolation of multiple serotypes. This information is crucial, particularly when trying to understand the complex ecology and epidemiology of Salmonella in livestock.To compare the culture methods for the isolatio...

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Section: Culture Methods Differ On the Isolation Of Salmonella Enterica Serotypes From Naturally Contaminated Swine Fecal Samplessupporting

confidence: 92%

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

et al. 2005

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“…These methods are based on the detection and amplification of specific DNA sequences of S. enterica, such as the invA gene, which encodes an invasion protein, or the rfb gene cluster, which encodes the O antigen [12]. Molecular methods can also provide information on the genetic diversity, phylogeny, and epidemiology of S. enterica serotypes in cattle [13].…”

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confidence: 99%

Accurate Identification of Salmonella enterica in Calves

Kabir,

Khan,

Ur Rahman

2024

Salmonella - Current Trends and Perspectives in Detection and Control

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Salmonella enterica is a bacterium that can cause serious infections in humans and animals, especially cattle. The identification and differentiation of S. enterica serotypes in cattle is important for epidemiological surveillance, disease prevention and control, and public health protection. However, the current methods and techniques for S. enterica detection have various challenges and limitations, such as low sensitivity and specificity, high cost and complexity, and the need for viable and pure bacterial cultures. Therefore, there is a need for further research and development of novel and improved methods and techniques that can overcome these challenges and provide reliable and accurate information on S. enterica serotypes in cattle. Such information can help to improve the understanding of the epidemiology, pathogenesis, and transmission of S. enterica in cattle, as well as to design and implement effective strategies for its prevention and control. This chapter reviews the current methods and techniques for S. enterica detection, such as culture-based methods, biochemical methods, molecular methods, phage-based methods, and biosensor methods, and discusses their advantages and disadvantages, as well as their future trends and perspectives.

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“…Many Salmonella serovars other than S. Choleraesuis cause food poisoning in human. Previous studies have shown that several serovars of Salmonella are prevalent among pigs [2,4,6,[11][12][13]. Hence, Salmonella prevalence among pigs causes a significant health problem for pigs and for people.…”

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confidence: 99%

“…Several studies have shown that the difference in results depends on the enrichment culture [6,7,9,11,20]. The discrepancy in isolation results between RV and HTT may occur in the case of isolation from feces or drag swabs, contaminated with other bacterial pathogens.…”

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Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

et al. 2003

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ABSTRACT. The growth of Salmonella Choleraesuis was examined in Rappaport Vassiliadis broth (RV) and Hajna-tetrathionate broth (HTT) at 37 and 42°C. As the enrichment in RV at 37°C was satisfactory for isolating S. Choleraesuis, we used this enrichment for isolation from the samples collected from 15 asymptomatic pigs reared on a S. Choleraesuis contaminated farm. S. Choleraesuis was frequently isolated from six pigs (40.0%) under field conditions. The isolation of other Salmonella serovars than S. Choleraesuis was attempted by using both RV enrichment at 37°C and HTT enrichment at 42°C. Salmonella organisms were isolated from 156 (44.8%) of 348 fecal samples and more frequently with HTT at 42°C (43.4%) than with RV at 37°C (20.9%). If other serovars in addition to S. Choleraesuis are to be surveyed, HTT enrichment should be used in combination with RV enrichment.

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Comparison of GN Hajna and Tetrathionate as Initial Enrichment for Salmonellae Recovery from Swine Lymph Nodes and Cecal Contents Collected at Slaughter

Cited by 8 publications

References 10 publications

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

Culture Methods Differ on the Isolation ofSalmonella EntericaSerotypes from Naturally Contaminated Swine Fecal Samples

Accurate Identification of Salmonella enterica in Calves

Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

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