Comparison of gpELISA and neutralizing antibody responses to Oka/Merck live varicella vaccine (Varivax®) in children and adults

Krah, David L.; Cho, Iksung; Schofield, T L; Ellis, Ronald W.

doi:10.1016/s0264-410x(96)00107-7

Cited by 43 publications

(24 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of a whole-antigen extract from VZV-infected cells containing a variety of VZV structural and nonstructural proteins has been shown as the most likely reason for that since a significantly greater sensitivity of ELISAs using purified viral gp antigen has been demonstrated (12,21). Thus, the gpELISA developed by the Merck Research Laboratories in the United States has a sensitivity comparable to that of FAMA, and the results agree with neutralizing antibodies (8). Unfortunately, this test is restricted to a small number of research laboratories and is not commercially available to date.…”

Section: Discussionmentioning

confidence: 97%

Serological Detection of Varicella-Zoster Virus-Specific Immunoglobulin G by an Enzyme-Linked Immunosorbent Assay Using Glycoprotein Antigen

Sauerbrei

Wutzler

2006

J Clin Microbiol

View full text Add to dashboard Cite

Since the introduction of varicella vaccination in several countries, there has been an urgent need for commercially available test procedures that allow highly sensitive and specific quantitative determination of the varicella-zoster virus (VZV)-specific immune status, including immunity postimmunization. This study compared the performance of two enzyme-linked immunosorbent assays (ELISAs) for the sensitive and specific determination of VZV-specific immunoglobulin G (IgG) in seronegative and latently infected persons, as well as in vaccinees. One ELISA is based on the detection of antibody to VZV-specific envelope glycoproteins (gp), and the other comprises the whole antigen extract prepared from VZV-infected cells. A modified standard fluorescent-antibody-to-membrane-antigen (FAMA) assay was used as a reference. An excellent sensitivity (100%) in relation to FAMA was demonstrated for the gpELISA (Virion\Serion), while the non-gpELISA (Dade Behring) had a lower sensitivity (83%) when sera from latently infected persons were tested. After postvaccinal immunity was measured, a sensitivity of 87% was achieved with gpELISA, whereas the ELISA incorporating antigen extract of VZV-infected cells had a sensitivity of 78%. Excellent specificity (100%) was calculated for both the gpELISA and the non-gpELISA. In conclusion, SERION ELISA classic VZV IgG is useful for the sensitive and specific quantitative determination of VZV immune status after natural infection. The test can also be recommended for measuring antibody response after varicella vaccination, particularly after the cutoff value was optimized.

show abstract

Section: Discussionmentioning

confidence: 97%

Serological Detection of Varicella-Zoster Virus-Specific Immunoglobulin G by an Enzyme-Linked Immunosorbent Assay Using Glycoprotein Antigen

Sauerbrei

Wutzler

2006

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…VZV produces at least seven glycoproteins, gK, gC, gB, gH, gL, gI, and gE, which are the products of ORFs 5,14,31,37,60,67,and 68,respectively (11). Two putative additional glycoproteins were recently described, gN (ORF 9A) and gM (ORF 50) (55,77).…”

Section: Vol 82 2008 Full-length Sequencing Of Two Oka Vaccine Stramentioning

confidence: 99%

“…Different sets of serological readouts have been used to characterize the adaptive humoral immune response after varicella vaccination or infection (4,13,26,31,34,58,73,74). Comparative analysis has raised the possibility that differences in the genetic code between the vaccine strains could be responsible for disparity in vaccine-induced humoral responses (36).…”

mentioning

confidence: 99%

Complete DNA Sequences of Two Oka Strain Varicella-Zoster Virus Genomes

Tillieux

Halsey

Thomas

et al. 2008

J Virol

View full text Add to dashboard Cite

show abstract

“…The gpELISAs are ELISAs that use the external glycoproteins (typically gE, gB, gH) as antigens of the solid phase [219,339,371,[404][405][406][407][408][409][410][411][412] . They are sensitive enough to detect low antibody levels after vaccination, and have been used to evaluate the level of protection [407] : In one study, 95% of the vaccinated subjects had a gpELISA value of ≥ 5 U/ mL six weeks after immunisation, whereas those with values of < 5 were at three times greater risk of developing BV [413] .…”

Section: Gpelisamentioning

confidence: 99%

Microbiology laboratory and the management of mother-child varicella-zoster virus infection

Paschale

Clerici

2016

WJV

View full text Add to dashboard Cite

Varicella-zoster virus, which is responsible for varicella (chickenpox) and herpes zoster (shingles), is ubiquitous and causes an acute infection among children, especially those aged less than six years. As 90% of adults have had varicella in childhood, it is unusual to encounter an infected pregnant woman but, if the disease does appear, it can lead to complications for both the mother and fetus or newborn. The major maternal complications include pneumonia, which can lead to death if not treated. If the virus passes to the fetus, congenital varicella syndrome, neonatal varicella (particularly serious if maternal rash appears in the days immediately before or after childbirth) or herpes zoster in the early years of life may occur depending on the time of infection. A Microbiology laboratory can help in the diagnosis and management of mother-child infection at four main times: (1) when a pregnant woman has been exposed to varicella or herpes zoster, a prompt search for specific antibodies can determine whether she is susceptible to, or protected against infection; (2) when a pregnant woman develops clinical symptoms consistent with varicella, the diagnosis is usually clinical, but a laboratory can be crucial if the symptoms are doubtful or otherwise unclear (atypical patterns in immunocompromised subjects, patients with post-vaccination varicella, or subjects who have received immunoglobulins), or if there is a need for a differential diagnosis between varicella and other types of dermatoses with vesicle formation; (3) when a prenatal diagnosis of uterine infection is required in order to detect cases of congenital varicella syndrome after the onset of varicella in the mother; and (4) when the baby is born and it is necessary to confirm a diagnosis of varicella (and its complications), make a differential diagnosis between varicella and other diseases with similar symptoms, or confirm a causal relationship between maternal varicella and malformations in a newborn. Core tip: Although varicella during pregnancy is infrequent and congenital varicella syndrome (CVS) is rare, every available means should be used to prevent and diagnose them. Microbiology laboratories can be crucial in these situations: Evaluating a mother's immune status with sensitive and specific tests for the detection of antibodies; allowing a rapid diagnosis with molecular biology tests when a clinical manifestation may be due to different etiologies; following pregnant women with varicella for the prenatal diagnosis of CVS with close collaboration between molecular biology investigators and specialists in imaging diagnostics.De Paschale M, Clerici P. Microbiology laboratory and the management of mother-child varicella-zoster virus infection. World

show abstract

Comparison of gpELISA and neutralizing antibody responses to Oka/Merck live varicella vaccine (Varivax®) in children and adults

Cited by 43 publications

References 20 publications

Serological Detection of Varicella-Zoster Virus-Specific Immunoglobulin G by an Enzyme-Linked Immunosorbent Assay Using Glycoprotein Antigen

Serological Detection of Varicella-Zoster Virus-Specific Immunoglobulin G by an Enzyme-Linked Immunosorbent Assay Using Glycoprotein Antigen

Complete DNA Sequences of Two Oka Strain Varicella-Zoster Virus Genomes

Microbiology laboratory and the management of mother-child varicella-zoster virus infection

Contact Info

Product

Resources

About