Chinese chive (
Allium tuberosum
Rottler) has potential risks associated with pathogenic bacterial contamination as it is usually consumed raw. In this study, we investigated the microbiota of Chinese chives purchased from traditional markets and grocery stores in March (Spring) and June (Summer) 2017. Differences in bacterial diversity were observed, and the microbial composition varied across sampling times and sites. In June, potential pathogenic genera, such as
Escherichia
,
Enterobacter
, and
Pantoea
, accounted for a high proportion of the microbiota in samples purchased from the traditional market. A large number of pathogenic bacteria (
Acinetobacter lwoffii
,
Bacillus cereus
,
Klebsiella pneumoniae
, and
Serratia marcescens
) were detected in the June samples at a relatively high rate. In addition, the influence of the washing treatment on Chinese chive microbiota was analyzed. After storage at 26°C, the washing treatment accelerated the growth of enterohemorrhagic
Escherichia coli
(EHEC) because it caused dynamic shifts in Chinese chive indigenous microbiota. These results expand our knowledge of the microbiota in Chinese chives and provide data for the prediction and prevention of food-borne illnesses.