Comparison of growth, neovascularization, and enzymatic function of fetal intestinal grafts in the omentum and renal capsule

Tisinai, Karen; Shedd, Frederick; Harris, Robert A.; Unthank, Joseph L.; Grosfeld, J L; Abu-Dalu, K; Grosfeld, Jay L.

doi:10.1016/0022-3468(90)90203-l

Cited by 15 publications

(4 citation statements)

References 5 publications

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“…Numerous studies have shown that fetal and newborn rat intestines, transplanted into some tissues of an adult syngeneic recipient as free grafts, can become vascularized, grow and mature both morphologically and functionally. 6,[8][9][10][11][12][13] The same results have been obtained in allogeneic intestinal transplantation under optimal doses of Cyclosporine A or FK 506 (tacrolimus). [14][15][16][17][18] Our study also confirmed the pos- sibility of fetal and newborn intestine transplantation as an avascular graft into the omentum of an adult recipient.…”

Section: Discussionmentioning

confidence: 75%

Techniques of intestinal transplantation in rat

et al. 1998

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Section: Discussionmentioning

confidence: 75%

Techniques of intestinal transplantation in rat

et al. 1998

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“…Implantation of small bowel in an omental wrap has resulted in better grafts than its implantation into subcutaneous tissue or renal capsule. [11][12] Functional integrity of the grafts following later anastomosis to the recipient's intestine was also demonstrated. 6,15 Winter et al 19 demonstrated that human fetal bowel matures when transplanted into a subcutaneous tunnel on the back of athymic nude mice.…”

Section: Discussionmentioning

confidence: 99%

“…It was the most used segment in other studies. 6,[10][11][12] It seemed easier to identify than the ileum. In the fetal and newborn intestine, jejunum's diameter was slightly larger than the ileum, which made the surgical technique easier.…”

Section: Discussionmentioning

confidence: 99%

Techniques of intestinal transplantation in rat

et al. 1998

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Two surgical models of intestinal transplantation in the rat are described. One is the implantation of fetal and newborn intestine as free grafts into the omentum of adult recipients, the other the adult intestine transplantation as an accessory graft using vascular anastomoses. A hundred and sixteen smallbowel transplantations were done; 36 of which were fetal intestine (group I), 40 of newborn intestine (group II), and 40 of adult intestine (group III). In the fetal and newborn intestinal transplantation, we emphasize the practices that allowed us to avoid ischemic and traumatic injury to the graft. In the adult intestine transplantation with vascular anastomoses, we heighten the modifications in the surgical technique that made the operation easier and the strategies used to prevent hypothermia and hypovolemic shock. Once experienced with the two chosen surgical techniques, transplantation using an avascular segment became much easier and quicker than transplantation with vascular anastomoses.Os autores descrevem dois modelos cirú rgicos de transplantaçã o intestinal no rato. Um corresponde à implantação de intestino de feto e de intestino de recé m-nascido, como enxertos livres, no epíploon de receptores adultos, e o outro corresponde à transplantaçã o de intestino de adulto, como enxerto acessório, usando anastomoses vasculares. Fizémos 116 transplantaçõ es intestinais: 36 de intestino fetal (grupo I), 40 de intestino de recé m-nascido (grupo II) e 40 de intestino de adulto (grupo III). Realçá mos os mé todos utilizados para evitar a isqué mia e a lesã o traumá tica do enxerto, nas transplantaçõ es de intestino de feto e de recé m-nascido. Na transplantaçã o de intestino de adulto com anastomoses vasculares, enfatizá mos as modificações na té cnica cirú rgica, que facilitaram a operaçã o, e as estraté gias usadas para evitar a hipotermia e o choque hipovolé mico. Apó s a familiarizaçã o com os dois modelos cirú rgicos, a transplantação intestinal como enxerto avascular revelou-se de execução mais simples e mais rá pida do que a transplantaçã o intestinal com realizaçã o de anastomoses vasculares.© 1998 Wiley-Liss, Inc. MICROSURGERY 18:424-429 1998Nowadays, the laboratory rat (Rattus norvegicus) is one of the most popular models for intestinal transplantation. Its use started with Monchick and Russell's work 1,2 in the 1970s, in which they applied the surgical techniques with vascular anastomoses, previously developed in bigger models by other investigators. 3,4 The intestinal transplantation technique varies according to the kind of graft and the investigation. While studying immunogenicity of fetal, newborn, and adult jejunal allografts in the rat, the authors used two methods of small-bowel transplantation. Fetal and newborn grafts were implanted as an avascular segment into the omentum of adult recipients. Adult allograft was transplanted to an adult recipient as a heterotopic accessory graft using aorta-aorta and porto-caval anastomoses. 5 The aim of this study is to review the intestinal transplantatio...

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“…These improved outcomes result from (a) the obliteration of dead space and the introduction of nonirradiated tissue, which improve perineal healing, and (b) the potential of VRAM and omental pedicle flaps to diminish adhesions between the pelvic floor and abdominal viscera (3). Omental pedicle flaps also have angiogenic and immunogenic properties that may potentially benefit recipient sites that have been irradiated or are infected (7)(8)(9).…”

Section: Introductionmentioning

confidence: 97%

Pelvic Reconstruction with Omental and VRAM Flaps: Anatomy, Surgical Technique, Normal Postoperative Findings, and Complications

Sagebiel¹,

Faria²,

Balachandran³

et al. 2011

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Soft-tissue reconstruction of the pelvis with vascularized tissue flaps has become an increasingly common procedure. The types of flaps and the indications for their use vary, but all flap procedures are performed with the goal of transferring tissue from a donor site to a recipient site to restore form and function, obliterate dead space, and create an environment favorable to wound healing. Oncologic surgeries, including total pelvic exenteration and abdominoperineal resection, are the leading indications for pelvic reconstruction. The pedicle flaps most commonly used for pelvic reconstruction following these oncologic interventions are the vertical rectus abdominis myocutaneous flap (VRAM) and the omental pedicle flap. Familiarity with the surgical techniques used for pelvic reconstruction with these flaps is crucial for the accurate interpretation of cross-sectional imaging studies, allowing the radiologist to distinguish between normal postoperative findings and complications or recurrent disease.

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Comparison of growth, neovascularization, and enzymatic function of fetal intestinal grafts in the omentum and renal capsule

Cited by 15 publications

References 5 publications

Techniques of intestinal transplantation in rat

Techniques of intestinal transplantation in rat

Techniques of intestinal transplantation in rat

Pelvic Reconstruction with Omental and VRAM Flaps: Anatomy, Surgical Technique, Normal Postoperative Findings, and Complications

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