Comparison of growth parameters of tiger puffer Takifugu rubripes from two culture systems in China

Wang, Qinglin; Zhang, Hongtao; Ren, Yuqin; Zhou, Qunlan

doi:10.1016/j.aquaculture.2015.11.022

Cited by 24 publications

(12 citation statements)

References 10 publications

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“…bimaculatus inhabits lower latitudes and adapts to higher temperatures than T . rubripes 2 , providing an excellent model for exploring thermal adaptation and adaptive divergence in teleost fishes. In the past decade, T .…”

Section: Background and Summarymentioning

confidence: 99%

The sequence and de novo assembly of Takifugu bimaculatus genome using PacBio and Hi-C technologies

et al. 2019

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Takifugu bimaculatus is a native teleost species of the southeast coast of China where it has been cultivated as an important edible fish in the last decade. Genetic breeding programs, which have been recently initiated for improving the aquaculture performance of T. bimaculatus, urgently require a high-quality reference genome to facilitate genome selection and related genetic studies. To address this need, we produced a chromosome-level reference genome of T. bimaculatus using the PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. The genome was assembled into 2,193 contigs with a total length of 404.21 Mb and a contig N50 length of 1.31 Mb. After chromosome-level scaffolding, 22 chromosomes with a total length of 371.68 Mb were constructed. Moreover, a total of 21,117 protein-coding genes and 3,471 ncRNAs were annotated in the reference genome. The highly accurate, chromosome-level reference genome of T. bimaculatus provides an essential genome resource for not only the genome-scale selective breeding of T. bimaculatus but also the exploration of the evolutionary basis of the speciation and local adaptation of the Takifugu genus.

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Section: Background and Summarymentioning

confidence: 99%

The sequence and de novo assembly of Takifugu bimaculatus genome using PacBio and Hi-C technologies

et al. 2019

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“…More interestingly, the rapid development of the fishing industry has resulted in a huge amount of collagen-rich by-products, including skin, scales, and bones (50.2–117 million metric tons) [10]. Pufferfish aquaculture is a thriving industry in China, producing more than 14,000 metric tons in 2013 [11]. Studies on fish suggest that pufferfish skin is rich in type I collagen and may serve as an appropriate source of collagen to replace their mammalian counterparts.…”

Section: Introductionmentioning

confidence: 99%

Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen

Chen

et al. 2019

Marine Drugs

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Collagen is widely used in drugs, biomaterials, foods, and cosmetics. By-products of the fishing industry are rich sources of collagen, which can be used as an alternative to collagen traditionally harvested from land mammals. However, commercial applications of fish-based collagen are limited by the low efficiency, low productivity, and low sustainability of the extraction process. This study applied a new technique (electrodialysis) for the extraction of Takifugu flavidus skin collagen. We found electrodialysis to have better economic and environmental outcomes than traditional dialysis as it significantly reduced the purification time and wastewater (~95%) while maintaining high extraction yield (67.3 ± 1.3 g/100 g dry weight, p < 0.05). SDS-PAGE, amino acid composition analysis, and spectrophotometric characterization indicated that electrodialysis treatment retained the physicochemical properties of T. flavidus collagen. Heavy metals and tetrodotoxin analyses indicated the safety of T. flavidus collagen. Notably, the collagen had similar thermal stability to calf skin collagen, with the maximum transition temperature and denaturation temperature of 41.8 ± 0.35 and 28.4 ± 2.5 °C, respectively. All evidence suggests that electrodialysis is a promising technique for extracting collagen in the fishing industry and that T. flavidus skin collagen could serve as an alternative source of collagen to meet the increasing demand from consumers.

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“…A high-quality genomic reference (i.e., assembled at the chromosome level) is now available (Kai et al 2011 ) and has been applied to various genetic studies (Kamiya et al 2012 ; Hosoya et al 2013 ; Ieda et al 2018 ). The species is also known for the high market price and is recognized as one of the important aquaculture species in East Asia (Wang et al 2016 ; Hamasaki et al 2017 ). Although the fish itself is valuable, a particularly prized part of the fish in Japan is the testes.…”

Section: Introductionmentioning

confidence: 99%

Genetic Dissection of a Precocious Phenotype in Male Tiger Pufferfish (Takifugu rubripes) using Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di)

et al. 2021

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The novel non-targeted PCR-based genotyping system, namely Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di), is characterized by the simplicity in library construction and robustness against DNA degradation and is expected to facilitate advancements in genetics, in both basic and applied sciences. In this study, we tested the utility of GRAS-Di for genetic analysis in a cultured population of the tiger pufferfish Takifugu rubripes. The genetic analyses included family structure analysis, genetic map construction, and quantitative trait locus (QTL) analysis for the male precocious phenotype using a population consisting of four full-sib families derived from a genetically precocious line. An average of 4.7 million raw reads were obtained from 198 fish. Trimmed reads were mapped onto a Fugu reference genome for genotyping, and 21,938 putative single-nucleotide polymorphisms (SNPs) were obtained. These 22 K SNPs accurately resolved the sibship and parent–offspring pairs. A fine-scale linkage map (total size: 1,949 cM; average interval: 1.75 cM) was constructed from 1,423 effective SNPs, for which the allele inheritance patterns were known. QTL analysis detected a significant locus for testes weight on Chr_14 and three suggestive loci on Chr_1, Chr_8, and Chr_19. The significant QTL was shared by body length and body weight. The effect of each QTL was small (phenotypic variation explained, PVE: 3.1–5.9%), suggesting that the precociousness seen in the cultured pufferfish is polygenic. Taken together, these results indicate that GRAS-Di is a practical genotyping tool for aquaculture species and applicable for molecular breeding programs, such as marker-assisted selection and genomic selection.

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Comparison of growth parameters of tiger puffer Takifugu rubripes from two culture systems in China

Cited by 24 publications

References 10 publications

The sequence and de novo assembly of Takifugu bimaculatus genome using PacBio and Hi-C technologies

The sequence and de novo assembly of Takifugu bimaculatus genome using PacBio and Hi-C technologies

Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen

Genetic Dissection of a Precocious Phenotype in Male Tiger Pufferfish (Takifugu rubripes) using Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di)

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