Comparison of Haliotis rubra hemocyanin isoforms 1 and 2

Wu, Jiadai; Cunningham, Anthony L.; Dehghani, Fariba; Diefenbach, Russell J.

doi:10.1016/j.genrep.2016.04.011

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…They were allowed to air dry at room temperature, and were then inserted in the mass spectrometer and subjected to mass analysis. The mass spectrometer was externally calibrated with a mixture of angiotensin I, Glu-fibrinopeptide B, ACTH (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17), and ACTH . For MS/MS experiments, the instrument was externally calibrated with fragments of Glu-fibrinopeptide B.…”

Section: Protein Identification By Ms and Ms/ms Analysesmentioning

confidence: 99%

“…Hemocyanins (Hcs), well-studied oligomeric glycometalloproteins carrying oxygen in the hemolymph of arthropod and mollusc species, differ largely in molecular masses, quaternary structures [1][2][3][4][5], carbohydrate content, and composition [6][7][8]. They have large molecular weights (from 4 up to 9 MDa) [4] and, due to their xenogenic nature, are strongly immunogenic.…”

Section: Introductionmentioning

confidence: 99%

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Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines

Dolashki

Dolashka

Stenzl

et al. 2019

Biotechnology & Biotechnological Equipment

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Hemocyanins are oxygen-transporting glycoproteins in molluscs and arthropods. In this study, we assayed the biomacromolecules from the molluscs Helix lucorum (HlH), Rapana venosa (RvH) and Megatura crenulata (KLH) including their functional units (FUs), for therapy of bladder cancer permanent cells. In vitro studies antitumour activities of these proteins were performed with bladder cancer permanent cell line CAL-29 and the normal urothelial cell line HL 10/29 in comparison to doxorubicin. The obtained results showed that the human tumour CAL-29 cell line is sensitive to the action of the tested hemocyanins and their isoforms. We observed a dose-and time-dependent inhibition of tumour cell growth after incubation with native HlH and two FUs (b c-HlH-a and FU b c-HlH-h); and of particular significance, FU b c-HlH-h showed a surprisingly stronger effect than that the doxorubicin-treated cells. Cells treated with b c-HlH-h, showed both, apoptotic and necrotic cells. In addition, two-dimensional polyacrylamide gel electrophoresis (PAGE) found for differential up-regulation of several proteins after hemocyanin treatment. Eight different down-regulated and two up-regulated proteins were identified, which may be associated with the apoptosis pathway. No inhibition of the normal urothelial cell line HL 10/29 was observed after treatment with HlH and its isoforms. The most effective inhibition of CAL-29 tumour cells was observed after treatment with b c-HlH-h, probably caused by a specific oligosaccharide structure of HlH with methylated hexoses. These results suggest that hemocyanin glycosylation plays an important role in its anticancer activity.

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Section: Protein Identification By Ms and Ms/ms Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines

Dolashki

Dolashka

Stenzl

et al. 2019

Biotechnology & Biotechnological Equipment

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“…Hence, the observed upregulation of the Hc isoform CaH αD in the midgut gland of Cu‐treated snails may reflect a role of the isoform in stress resistance, rather than indicating a direct connection between an increased Cu supply and Hc synthesis. In fact, intraspecific multiplicity of Hc isoforms may translate into functional specification of single isoforms, including their involvement in stress resistance like innate immunity, as well as antiviral or antibacterial activity (Wu et al, 2016; Yao et al, 2019). In the cuttlefish Sepia officinalis , several Hc genes are expressed differentially depending on the developmental stage and on adaptation to external stressors such as temperature of hypercapnia (Strobel et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Responsiveness of metallothionein and hemocyanin genes to cadmium and copper exposure in the garden snailCornu aspersum

et al. 2020

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Terrestrial gastropods express metal‐selective metallothioneins (MTs) by which they handle metal ions such as Zn2+, Cd2+, and Cu+/Cu2+ through separate metabolic pathways. At the same time, they depend on the availability of sufficient amounts of Cu as an essential constituent of their respiratory protein, hemocyanin (Hc). It was, therefore, suggested that in snails Cu‐dependent MT and Hc pathways might be metabolically connected. In fact, the Cu‐specific snail MT (CuMT) is exclusively expressed in rhogocytes, a particular molluscan cell type present in the hemocoel and connective tissues. Snail rhogocytes are also the sites of Hc synthesis. In the present study, possible interactions between the metal‐regulatory and detoxifying activity of MTs and the Cu demand of Hc isoforms was explored in the edible snail Cornu aspersum, one of the most common European helicid land snails. This species possesses CdMT and CuMT isoforms involved in metal‐selective physiological tasks. In addition, C. aspersum expresses three different Hc isoforms (CaH ɑD, CaH ɑN, CaH β). We have examined the effect of Cd2+ and Cu2+ exposure on metal accumulation in the midgut gland and mantle of C. aspersum, testing the impact of these metals on transcriptional upregulation of CdMT, CuMT, and the three Hc genes in the two organs. We found that the CuMT and CaH ɑD genes exhibit an organ‐specific transcriptional upregulation in the midgut gland of Cu‐exposed snails. These results are discussed in view of possible interrelationships between the metal‐selective activity of snail MT isoforms and the synthesis and metabolism of Hc isoforms.

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Recovery of high-value compounds from food by-products

Blackshaw

Cho

et al. 2022

Food Engineering Innovations Across the Food Supply Chain

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Comparison of Haliotis rubra hemocyanin isoforms 1 and 2

Cited by 4 publications

References 45 publications

Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines

Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines

Responsiveness of metallothionein and hemocyanin genes to cadmium and copper exposure in the garden snailCornu aspersum

Recovery of high-value compounds from food by-products

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