Comparison of HbA1c measurement methods between automatic boronate affinity point of care testing and high-performance liquid chromatography

Tjahjadi, Ricky; Giantini, Astuti

doi:10.15562/bmj.v8i1.1346

Cited by 1 publication

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“…The fresh samples were assayed using boronate affinity HPLC, while the frozen ones were assayed using cation-exchange HPLC. Although several studies 22,26 had crossvalidated both assay methods and a high correlation (r = 0.993) and interclass correlation coefficient (ICC = 0.961) (File S1) were observed in our samples, assay instrumentation changes might contribute to the variation in HbA1c measurements. In addition, other confounding factors, such as different blood collection tubes and the additives added to preserve the biospecimens, might influence the measurement.…”

Section: Discussionmentioning

confidence: 95%

“…Although this study used two different systems for HbA1c measurement, previous studies indicate no systemic and proportional differences between HPLC systems of boronate affinity and cation exchange 26 . However, we still did a pilot study to assess their correlation using fresh blood samples ( n = 41).…”

Section: Methodsmentioning

confidence: 95%

“…25 Although this study used two different systems for HbA1c measurement, previous studies indicate no systemic and proportional differences between HPLC systems boronate affinity and cation exchange. 26 However, we still did a pilot study to assess their correlation using fresh blood samples (n = 41). The Pearson's correlation and interclass correlation coefficient (ICC) between Premier Hb9210 and Variant™ II Turbo analyzers were high (r = 0.993 and ICC = 0.96, respectively), with a regression formula of y = 0.95x + 0.58 (Supplementary material S1).…”

Section: Hba1c Measurementmentioning

confidence: 99%

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Stability of glycated haemoglobin (HbA1c) measurements from whole blood samples kept at −196°C for seven to eight years in The Malaysian Cohort study

Abdullah

Goh

Othman

et al. 2023

Clinical Laboratory Analysis

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Objective Glycated haemoglobin (HbA1c) is a standard indication for screening type 2 diabetes that also has been widely used in large‐scale epidemiological studies. However, its long‐term quality (in terms of reproducibility) stored in liquid nitrogen is still unknown. This study is aimed to evaluate the stability and reproducibility of HbA1c measurements from frozen whole blood samples kept at −196°C for more than 7 years. Methods A total of 401 whole blood samples with a fresh HbA1c measurement were randomly selected from The Malaysian Cohort's (TMC) biobank. The HbA1c measurements of fresh and frozen (stored for 7–8 years) samples were assayed using different high‐performance liquid chromatography (HPLC) systems. The HbA1c values of the fresh samples were then calculated and corrected according to the later system. The reproducibility of HbA1c measurements between calculated‐fresh and frozen samples was assessed using a Passing‐Bablok linear regression model. The Bland–Altman plot was then used to evaluate the concordance of HbA1c values. Results The different HPLC systems highly correlated (r = 0.99) and agreed (ICC = 0.96) with each other. Furthermore, the HbA1c measurements for frozen samples strongly correlate with the corrected HbA1c values of the fresh samples (r = 0.875) with a mean difference of −0.02 (SD: −0.38 to 0.38). Although the mean difference is small, discrepancies were observed within the diabetic and non‐diabetic samples. Conclusion These data demonstrate that the HbA1c measurements between fresh and frozen samples are highly correlated and reproducible.

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Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 95%