Comparison of Helicobacter pylori Detection Using Immunohistochemistry and Giemsa and Its Association with Morphological Changes in Active Chronic Gastritis

Nurdin, Wildawati; Krisnuhoni, Ening; Kusmardi, Kusmardi

doi:10.24871/171201621-27

Cited by 5 publications

(10 citation statements)

References 14 publications

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“…All the patients (with or without treatment) were reported with IHC, as it is an antigen/antibody-based reaction in which antibodies react with the somatic antigen of bacteria. The patients are diagnosed with active and chronic gastritis with mild and moderate colonization, most patients are reported as having mild active chronic gastritis and our results are supported by the study of Nurdin et al (2016). The patients are grouped into different age groups, males within the most prominent group 21-30 and females 31-40), these two groups are severely affected, Dogar and Qureshy (2012) also concluded the same results in their study.…”

Section: Identification Of Hpylori By Handesupporting

confidence: 84%

“…49 cases were negative among all age groups of male patients. Normal Mucosa was seen as shown in figures 14 Immunohistochemistry was carried out with Helicobacter pylori immunostain, and all the 66 cases which were initially screened as positive by H&E were positive by IHC and the 22 cases which were negative on H&E were also positive by IHC (Table 1). Among our 210 cases, there were 51% males, and 49% females (Table 2).…”

Section: Identification Of Hpylori By Handementioning

confidence: 99%

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Applications Of The Immune-Histochemical Technique For Diagnosis Of Helicobacter Pylori From Gastric Biopsy Samples

Larik¹

2023

Journal of Pharmaceutical Negative Results

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Section: Identification Of Hpylori By Handesupporting

confidence: 84%

Section: Identification Of Hpylori By Handementioning

confidence: 99%

Applications Of The Immune-Histochemical Technique For Diagnosis Of Helicobacter Pylori From Gastric Biopsy Samples

Larik¹

2023

Journal of Pharmaceutical Negative Results

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“…Worldwide, the incidence of gastritis was 1.8-2.1 million, while in South East Asia, 583,635 per year. The incidence of gastritis in Indonesia itself is very high, which is 247,396 cases from 238.452.952 population [1]. Gastritis is defined as the histological presence of gastric mucosal inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…H. pylori infection plays an important role in the development of peptic ulcer in gastritis patients. H. pylori infection in developing countries was about 25-30%, where 5-27% were found in early childhood and 50-60% were found in adults aged more than 60 years old [1]. H. pylori reside within the mucous layer.…”

Section: Introductionmentioning

confidence: 99%

The Difference of Serum Gastrin-17 Level Based on Gastritis Severity and Helicobacter Pylori Infection

Parhusip¹,

Siregar²,

Dairi³

2019

Open Access Maced J Med Sci

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BACKGROUND: Gastritis was defined as the histological presence of gastric mucosal inflammation. One of the most common aetiology was H. pylori. Gastrin-17 was a hormone that was secreted by G cells. H. pylori infection induced increased in gastrin-17 in gastritis. Therefore, this study was to investigate the relationship of gastrin-17 with gastritis severity and H. pylori infection. AIM: To determine the difference in serum Gastrin-17 level based on gastritis severity and H. pylori infection. METHODS: A cross-sectional study enrolling 45 patients with gastritis was conducted in Haji Adam Malik General Hospital between April and July 2018. Endoscopy and biopsy examinations were performed to confirm the diagnosis of gastritis. Gastritis severity was assessed using the Updated Sydney System. The presence of H. pylori infection was detected by a Campylobacter-like organism (CLO) examination. Gastrin-17 level and demographic data were also gathered. The analysis was done using Mann Whitney and Kruskal-Wallis test. P-value of < 0.05 was considered statistically significant. RESULTS: Serum Gastrin-17 level was significantly different based on gastritis severity (P = 0.001 according to neutrophils infiltration and P = 0.023 according to degree of atrophy), H. pylori infection (P = 0.038), and combined gastritis severity and H. pylori infection (P < 0.001). Serum Gastrin-17 level was higher in subjects with severe neutrophils infiltration, without atrophy, and with H. pylori infection. CONCLUSION: There was a significant difference in serum Gastrin-17 level based on gastritis severity and H. pylori infection.

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“…Generally, the prevalence of H. pylori infection varies demographically. Although the infection may affect all ages, adults ≥ 60 years of age were rather prone to the infection ( 4 ). H. pylori can cause infection by multiple routes; however, the ingestion of bacteria is speculated as primary mode of transmission from one person to another ( 5 ), which might be attributed to the consumption of bacteria-tainted water and failure to practice proper hygiene ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Warthin-Starry Staining for the Detection of Helicobacter pylori in Gastric Biopsies

Farouk

Hassan

Ismail

et al. 2018

MJMS

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BackgroundWarthin-Starry (WS) staining is an ancillary stain used in the detection of Helicobacter sp., spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of H. pylori diagnosis in gastric biopsies in comparison with anti-H. pylori immunohistochemistry (IHC) staining.MethodsThis study involved 104 cases of gastric biopsies that were previously subjected to WS staining. All cases involved retrieval of formalin-fixed paraffin-embedded (FFPE) gastric biopsies that were re-cut, subjected to anti-H. pylori IHC staining and reviewed blindly by a pathologist. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of WS as compared to IHC were calculated.ResultsIn this study, WS stain was less sensitive in detecting H. pylori. The sensitivity, specificity, PPV and NPV for WS stain were 50.0%, 92.4%, 79.2% and 76.3%, respectively.ConclusionsThe sensitivity of WS stain in the histopathology laboratory was lower than that described previously. Several external factors that might influence the results were identified. However, sufficient information on patients’ history of treatment and medication would be required for the diagnosis or confirmation of the presence of H. pylori in gastric biopsies by WS staining.

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Comparison of Helicobacter pylori Detection Using Immunohistochemistry and Giemsa and Its Association with Morphological Changes in Active Chronic Gastritis

Abstract: Background: the gaster. The most common aetiology of chronic gastritis is Helicobacter pylori (H. pylori) infection. Presence

Cited by 5 publications

References 14 publications

Applications Of The Immune-Histochemical Technique For Diagnosis Of Helicobacter Pylori From Gastric Biopsy Samples

Applications Of The Immune-Histochemical Technique For Diagnosis Of Helicobacter Pylori From Gastric Biopsy Samples

The Difference of Serum Gastrin-17 Level Based on Gastritis Severity and Helicobacter Pylori Infection

Warthin-Starry Staining for the Detection of Helicobacter pylori in Gastric Biopsies

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