Comparison of heparin-immobilized vs. antibody-immobilized microspheres for the capture and detection of cytokines during microdialysis sampling

Duo, Jia; Cabrera, Randy F. Espinal; Stenken, Julie A.

doi:10.1109/lissa.2009.4906722

Cited by 1 publication

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Paired t tests ( p <0.05) between the control and the heparin-immobilized microsphere-containing probes show that the RR values are statistically different for aFGF at the flow rate of 0.5 μL/min, VEGF at all three flow rates, CCL2 at the flow rates of 1.0 and 2.0 μL/min, and CCL5 at the flow rates of 0.5 and 1.0 μL/min. An average of two to fivefold increase of RR was achieved for the four cytokines using these heparin-immobilized microspheres in the perfusion fluid, which is similar to that for antibodies [35]. …”

Section: Resultsmentioning

confidence: 95%

In vitro and in vivo affinity microdialysis sampling of cytokines using heparin-immobilized microspheres

Duo

Stenken

2010

Anal Bioanal Chem

Self Cite

View full text Add to dashboard Cite

Heparin-immobilized microspheres were included in microdialysis sampling perfusion fluids under both in vitro and in vivo conditions to improve the recovery of different cytokines, acidic fibroblast growth factor, vascular endothelial growth factor, monocyte chemoattractant protein-1 (or CCL2), and regulation upon activation normal T cell express sequence (or CCL5). Different strategies to dissociate captured CCL2 and CCL5 from the immobilized heparin were attempted, and both cytokines could be quantitatively eluted from the beads using a phosphate buffer (pH 7.4) containing 25% (v/v) acetonitrile which did not interfere with the subsequent detection of cytokine using an ELISA assay. Using these heparin-immobilized microspheres, a two to fivefold increase of microdialysis relative recovery (RR) was achieved for the four cytokines from a quiescent solution. Enhanced microdialysis RR of CCL2 using the heparin-immobilized microspheres from microdialysis probes implanted into the peritoneal cavity of a rat was performed to test the in vivo application. This work suggests that the heparin-immobilized microspheres provide an alternative affinity agent to the previously used antibody-immobilized microspheres for enhanced microdialysis sampling of cytokines.

show abstract

Section: Resultsmentioning

confidence: 95%