Comparison of heterotrophic and photoautotrophic induction on astaxanthin production by Haematococcus pluvialis

Kang, Chang Duk; Lee, J. S.; Park, T. H.; Sim, Sang Jun

doi:10.1007/s00253-005-1889-2

Cited by 149 publications

(73 citation statements)

References 23 publications

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“…Capabilities in utilization of inorganic carbons cannot be restored by acclimating cells under moderate light and either with N or without N added. This conclusion differed from the previous findings that H. pluvialis cells prefer to assimilate CO 2 for biomass and astaxanthin biosynthesis (Kang et al, 2005). The difference may arise from the distinguished genetic make‐up among different H. pluvialis strains, but also probably due to a metabolic shift caused by culture conditions.…”

Section: Discussioncontrasting

confidence: 99%

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Zhang

Wang

et al. 2016

Biotech & Bioengineering

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The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high‐value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark‐grown cells. Although moderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5 mg L−1 day−1) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark‐grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high‐light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5 mg L−1 day−1 by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark‐grown cells under photo‐induction conditions. Biotechnol. Bioeng. 2016;113: 2088–2099. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

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Section: Discussioncontrasting

confidence: 99%

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Zhang

Wang

et al. 2016

Biotech & Bioengineering

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“…A heterophotric-photoautotrophic culture mode was also explored where heterotrophic culture produced algal biomass, while astaxanthin production was induced during photoautotrophic culture. As a result, a very high cellular astaxanthin content of 7% by dwt, but low astaxanthin productivities of 4.4-6.5 mg L -1 d -1 were obtained (Hata et al 2001, Kang et al 2005. tion (Choi et al 2002).…”

Section: Metabolic Engineering For Enhanced Carotenoid Productionmentioning

confidence: 99%

Astaxanthin in microalgae: pathways, functions and biotechnological implications

Han¹,

Li²,

Hu³

2013

ALGAE

185

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Major progress has been made in the past decade towards understanding of the biosynthesis of red carotenoid astaxanthin and its roles in stress response while exploiting microalgae-based astaxanthin as a potent antioxidant for human health and as a coloring agent for aquaculture applications. In this review, astaxanthin-producing green microalgae are briefly summarized with Haematococcus pluvialis and Chlorella zofingiensis recognized to be the most popular astaxanthin-producers. Two distinct pathways for astaxanthin synthesis along with associated cellular, physiological, and biochemical changes are elucidated using H. pluvialis and C. zofingiensis as the model systems. Interactions between astaxanthin biosynthesis and photosynthesis, fatty acid biosynthesis and enzymatic defense systems are described in the context of multiple lines of defense mechanisms working in concert against photooxidative stress. Major pros and cons of mass cultivation of H. pluvialis and C. zofingiensis in phototrophic, heterotrophic, and mixotrophic culture modes are analyzed. Recent progress in genetic engineering of plants and microalgae for astaxanthin production is presented. Future advancement in microalgal astaxanthin research will depend largely on genome sequencing of H. pluvialis and C. zofingiensis and genetic toolbox development. Continuous effort along the heterotrophic-phototrophic culture mode could lead to major expansion of the microalgal astaxanthin industry.

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“…Haematococcus accumulates astaxanthin when exposed to disadvantageous environmental factors, such as nitrogen starvation, strong illumination, high values of temperature, pH or salt concentration and phosphorus deficit (Fabregas et al, 2003;Kang et al, 2005) and forms thickwalled cells impregnated with algaenan, an acetolysis-resistant biopolymer (Montsant et al, 2001). The addition of sodium acetate, L-asparagine, trace elements and vitamin B causes an increase in biomass production and total astaxanthin production during a short time under both autotrophic and mixotrophic conditions (Tripathi et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Assimilation of various organic carbon sources by Haematococcus strains

Zych

Stolarczyk

Maca

et al. 2009

Acta Agronomica Hungarica

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Differences in the assimilation of individual organic compounds (5 mM sugars and L-asparagine) under mixotrophic growth conditions were described for three naturally occurring Haematococcus strains.The effects of assimilation were measured by the growth intensity and size of algal cells, and the effect of colour changes in the cultures was observed. Some compounds caused the cell colouration to change from green to yellow, being the result of chlorophyll disappearance and the accumulation of yellow secondary carotenoids. In the present experiment none of the cultures turned red, thus excluding the intense accumulation of the commercially interesting carotenoid, astaxanthin.

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Comparison of heterotrophic and photoautotrophic induction on astaxanthin production by Haematococcus pluvialis

Cited by 149 publications

References 23 publications

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Astaxanthin in microalgae: pathways, functions and biotechnological implications

Assimilation of various organic carbon sources by Haematococcus strains

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