Comparison of high-performance liquid chromatography with fluorescence polarization immunoassay for the analysis of vancomycin in patients with chronic renal failure

Clinical Laboratory–Based Assay Methodologies May Underestimate and Increase Variability of Vancomycin Protein Binding in Hospitalized Patients

“…Although these methods offer sufficient sensitivity and selectivity for clinical purposes, they were developed and validated for anticipated total drug serum concentrations. Consistent with our findings, studies in the analytical literature have shown a trend toward an overprediction of vancomycin concentrations with these methods, particularly with concentrations on the lower end of the linear range . Although the clinical significance of the noted differences in protein binding between the two methods is debatable, use of the FPIA assay certainly results in an underestimation of protein binding.…”

Vancomycin assay performance in patients with acute renal failure

“…Numerous studies have shown that pFPIA significantly overestimates vancomycin serum concentrations compared to EMIT [6±8] or high-performance liquid chromatography (HPLC) [7,9,10] in patients with chronic renal dysfunction. However, recent reports suggest that the FPIA-based vancomycin assays conducted on the TDx system no longer overestimate vancomycin serum concentrations compared to EMIT [11] or HPLC [12].…”

Analytical methods for vancomycin determination in biological fluids and in pharmaceuticals

“…This could be a consequence, among other factors, of the interference of crystalline degradation products 17,[24][25][26][27][28][29][30][31][32] .…”