Comparison of Histamine and 5-Hydroxytryptamine Content and Secretion in Rat Mast Cells Isolated from Different Anatomical Locations

Abstract

Rat mast cells, isolated from different anatomical locations, were found to exhibit heterogeneity with respect to histamine and 5-hydroxytryptamine (5-HT) content. Unlike peritoneal mast cells, those derived by enzymatic digestion of rat lung and mesentery tissues contained amounts of 5-HT in excess of histamine. Both compound 48/80 and A23187 were more effective liberators of histamine than 5-HT in each cell type, despite their differences in relative amine content. However, in response to a mixture of specific antigen and phosphatidylserine, the pattern of secretion of both amines from each mast cell type was observed to be essentially collinear. These results are discussed in terms of amine release mechanisms and post-secretory events.

Facial injections of pruritogens or algogens elicit distinct behavior responses in rats and excite overlapping populations of primary sensory and trigeminal subnucleus caudalis neurons

“…Cheek injection of 5-HT was presently found to elicit dose-dependent hindlimb scratching, consistent with previous reports that 5-HT evoked hindlimb scratch bouts directed to the injection site in the nape of the neck in rats (Hachisuka et al 2010;Jinks and Carstens 2002;Carstens 2003a, 2003b;Nojima et al 2003;Thomsen et al 2001Thomsen et al , 2002 and mice (Inagaki et al 2001;Maekawa et al 2000;Yamaguchi et al 1999). Rat mast cells contain higher concentrations of 5-HT than histamine (Purcell et al 1989), possibly related to the scratch-inducing capacity of 5-HT in this species. 5-HT elicited relatively few forelimb wipes, with only one concentration of 5-HT evoking significantly more wipes compared with vehicle control animals ( Table 1).…”

5-Hydroxytryptamine (5-HT)2Receptor Involvement in Acute 5-HT-Evoked Scratching but Not in Allergic Pruritus Induced by Dinitrofluorobenzene in Rats

“…Intradermal injection of other agents that induce itch in humans, such as histamine, compound 48/80, substance P, and proteases, elicited little or no scratching in Sprague-Dawley rats (Thomsen et al, 2001;Jinks and Carstens, 2002). Rodent mast cells contain relatively high concentrations of 5-HT (Gustafsson, 1980;Graziano, 1988;Purcell et al, 1989), consistent with a role for 5-HT as an itch mediator in rodents.…”

Glucosylsphingosine Induces Itch-Scratch Responses in Mice

“…This finding was indicative of the involvement of the serotonin-mediated pathway in Gsp-induced ISRs. Serotonin has been reported to be an important pruritogen in mice, because dermal mast cells in rodents contain little histamine but high concentrations of serotonin (Purcell et al, 1989). It is also reported that serotonin receptors such as 5HT1 and 5-HT2 receptors exist in the peripheral nerve endings (Kim et al, 2008a).…”