Comparison of histamine release induced by synthetic polycations with that by compound 48/80 from rat mast cells.

Abstract

Abstract-We compared the histamine release induced by polyethylenimines and polyallylamines with that induced by compound 48/80.Lidocaine inhibited the histamine release induced by polyethylenimine with a molecular weight of 600 (PEI6), but disodium cromoglycate did not. The histamine releases induced by all polyethylenimines and polyallylamines tested were inhibited by lidocaine, but not by disodium cromoglycate.islet activating protein inhibited the histamine release induced by PEI6. Its effects on the release by other polyethylenimines and polyallylamines were less than that on PEI6. It is likely that the inhibition of G proteins by islet activating protein resulted in a decrease of the histamine release. This possibility was supported by the finding that guanyl-5'-(8,r-imino) triphos phate enhanced the histamine release.An inhibitor of polyphosphoinositide phos phodiesterase, neomycin, did not affect the histamine releases induced by these polymers.The effect of PEI6 seemed to resemble that of compound 48/80. After pretreatment of mast cells with wheat germ agglutinin and with Limax flavus ag glutinin, releases of histamine induced by PEI6 and compound 48/80 decreased, suggesting that the binding sites of PEI6 and compound 48/80 had sialic acid and/ or N-acetyl glucosamine residues. The binding site for PEI6 seemed to especially overlap those of compound 48/80.

Sugar-Specific Inhibitory Effects of Wheat Germ Agglutinin and Phytohemagglutinin-E4 on Histamine Release Induced by Basic Secretagogues from Rat Peritoneal Mast Cells and Their Possible Action Sites

“…Moreover, histamine re lease induced by compound 48/80 or brady kinin is inhibited by pertussis toxin, but that induced by antigen or anti-IgE is not (2)(3)(4). We found that the histamine release induced by polyethylenimine (MW = 600, PEI6) was inhibited by pertussis toxin (5). Compound 48/80, bradykinin and polyethylenimine are all basic secretagogues.…”

“…It is well-known that concanavalin A (Con A) induces histamine release from mast cells in the presence of extracellular calcium and phosphatidylserine, because it dimerizes IgE receptors on the mast cells, as do antigens (10). However, Con A, in the absence of phosphatidylserine, did not affect the hista mine release induced by compound 48/80 or PEI6 (5). From rat peritoneal mast cells, sub stance P induces histamine release, which is inhibited by pertussis toxin (9).…”

Correlating animal and human phase Ia/Ib clinical data with CALAA-01, a targeted, polymer-based nanoparticle containing siRNA

“…The delivery components alone resulted in a 10-fold higher histamine release than CALAA-01. Uchida et al have shown how cationic polymers can induce histamine release in rat mast cells (22). The histamine release following CALAA-01 was mitigated by standard pretreatment with antihistamines and steroids.…”

Datura stramonium Agglutinin Released Histamine from Rat Peritoneal Mast Cells That Was Inhibited by Pertussis Toxin, Haptenic Sugar and N-Acetylglucosamine-Specific Lectins: Involvement of Glycoproteins with A-Acetylglucosamine Residues

“…(1, 2), substance P, bradykinin (3,4) and PEI6 (5,6) is independent of IgE receptors and extracellular calcium, but dependent upon Gi-like G protein activation. Rat peritoneal mast cells are a model system for the study of human skin mast cells as both cells respond to both IgE receptor-dependent and non-immunologic stimuli (7).…”