Comparison of histologic, biochemical, and mechanical properties of murine skin treated with the 1064‐nm and 1320‐nm Nd:YAG lasers

Dang, Yongyan; Ren, Qiushi; Liu, Huaxu; Ma, Jian; Zhang, Jinsheng

doi:10.1111/j.1600-0625.2005.00371.x

Cited by 35 publications

(27 citation statements)

References 28 publications

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“…For the other lasers, especially for 1320-nm laser treatment, the increase of collagen type I overpasses markedly collagen type III. We thus assumed the photo -mechanical reaction was helpful to synthesize collagen type III while the photo-thermal effect benefited the synthesis of collagen type I [22]. As the Q-switched 1064-nm laser induced more collagen formation than the 1320-nm laser, especially collagen type III, it was assumed collagen type III was very important in skin remodeling even if it was less in the dermis [22].…”

Section: ⅳDiscussionmentioning

confidence: 99%

“…Upon that, we select KM mice as our animal model, which is cheap, easily feeded and has been accepted [22]. Based on the theory of selective photothermolysis, we applied four commonly used lasers which target differently in our study.…”

Section: ⅳDiscussionmentioning

confidence: 99%

“…The quantitative analysis of hydroxyproline was carried out according to the method described by Dang et al [22].…”

Section: Quantitative Analysis Of Hydroxyprolinementioning

confidence: 99%

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Non-ablative collagen remodeling initiated by different laser effects: a comparative study on mouse model

et al. 2007

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Many lasers have claimed the clinical efficacy on skin rejuvenation. Systematic and comparative studies are needed to compare different laser effects and probe into the mechanism of laser skin rejuvenation. We performed this study to compare collagen remodeling with different laser effects on mice model in vivo. After depilation, the back skin of KM mice was used for the study. The 595nm pulsed dye laser (10ms), 1320nm Nd:YAG laser(350 µs), 1064nm Q-switched (5ns) and long-pulsed Nd:YAG(0.3ms) lasers were applied based on optimal tissue reaction fluence test to irradiate one side of the mice back and leave the other side as the control. Then the collagen remodelling was evaluated at 0, 1, 7, 21, 30 and 60 days, with biophysical parameters' measurements, histological and biochemical examination. All lasers applied showed a statistical improvement in skin elasticity, dermal thickness and synthesis of hydroxyproline compared with their own controls. The Q-switched 1064-nm laser resulted in greater improvement of skin elasticity, dermal thickness, and higher synthesis of hydroxyproline than the other lasers after two months of treatments, while there was no significant difference among the 595nm, 1320nm and long-pulsed 1064nm lasers. Collagen type III increased markedly after the Q-switched 1064-nm laser treatment whereas more collagen type I was elicited by the 1320-nm laser. .INTRODCTION SKIN aging is increasingly becoming a severe cosmetic disfigurement which may cause psychologic damage and prompt patients to seek advice about treatment. Treatment options include massage therapy, microdermabrasion, topical skin care products, chemical peels, and laser resurfacing [1-3]. CO2 and Er:YAG lasers, although effective in wrinkle reduction, are associated with a significant degree adverse effects such as bleeding, infection and hyper-or hypo-pigmentations[4-5]. With the desire for greater efficacy and minimal down-time, non-ablative lasers have been introduced to rejuvenate facial skin [6]. Non-ablative skin rejuvenation involved in delivering a useful packet of photothermal damage to the upper dermis under a cooled epidermis, thereby initiating the wound healing process with little risk of epidermal damage and achieving the required collagen remodeling. The first reported attempt at nonablative resurfacing was by Goldberg with the Q-switched 1064-nm Nd:YAG laser in 1997 [6]. He found that the QS Nd:YAG laser provides satisfactory clinical results with no post-operative morbidity Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/22/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx in the treatment of periocular and perioral rhytides. The 1064-nm QS laser creates a mechanical wave impact on the cutaneous surface and the thermal effect is practically nonexistent, with no effects on adjacent areas. Moreover, 1064 nm is not well absorbed in water and the penetration in tissue is thus deeper so that damage could probably not be limited to the superficial and mid-dermis. Goldberg performed ser...

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Section: ⅳDiscussionmentioning

confidence: 99%

Section: ⅳDiscussionmentioning

confidence: 99%

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Non-ablative collagen remodeling initiated by different laser effects: a comparative study on mouse model

et al. 2007

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“…The Nd:YAG laser wavelength of 1064 nm is associated with increased collagen deposition in the papillary and upper reticular dermis [11][12][13][14] and it has also been suggested that this wavelength may lead to a deeper dermal wound that can be utilized for nonablative dermal remodeling. 10) In addition, heat-induced protein (hsp) 70 and procollagen 1 have been reported to be expressed in dermal dendritic cells and we suggest that these cells may participate in the deposition of dermal collagen after the treatment.…”

Section: Discussionmentioning

confidence: 99%

“…[10][11] Many authors reported that laser treatment at this wavelength increased homogenization of the collagen through the penetration of this wavelength into the deeper dermis and its stimulation of the wound healing mechanisms with the formation of controlled zones of mild photothermal damage. [11][12][13][14] This was argued to trigger wound healing-related neocollagenesis from the dermal fibroblasts in the mid-to upper dermis, followed by the remodeling phase to give a tight, well-organized dermal matrix under a younger looking epidermis. The present study was designed to evaluate and compare the efficacy between the micropulsed and Q-switched modes of the 1064 nm Nd:YAG laser in the treatment of enlarged pores.…”

Section: Roh Mr Et Almentioning

confidence: 99%

TREATMENT OF ENLARGED PORES WITH THE QUASI LONG-PULSED VERSUS Q-SWITCHED 1064nm Nd:YAG LASERS: A SPLIT-FACE, COMPARATIVE, CONTROLLED STUDY

et al. 2011

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Enhanced absorption of millimeter wave energy in murine subcutaneous blood vessels

Алексеев

Ziskin

2011

Bioelectromagnetics

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The aim of the present study was to determine millimeter wave (MMW) absorption by blood vessels traversing the subcutaneous fat layer of murine skin. Most calculations were performed using the finite-difference time-domain (FDTD) technique. We used two types of models: (1) a rectangular block of multilayer tissue with blood vessels traversing the fat layer and (2) cylindrical models with circular and elliptical cross sections simulating the real geometry of murine limbs. We found that the specific absorption rate (SAR) in blood vessels normally traversing the fat layer achieved its maximal value at the parallel orientation of the E-field to the vessel axis. At 42 GHz exposure, the maximal SAR in small blood vessels could be more than 30 times greater than that in the skin. The SAR increased with decreasing the blood vessel diameter and increasing the fat thickness. The SAR decreased with increasing the exposure frequency. When the cylindrical or elliptical models of murine limbs were exposed to plane MMW, the greatest absorption of MMW energy occurred in blood vessels located on the lateral areas of the limb model. At these areas the maximal SAR values were comparable with or were greater than the maximal SAR on the front surface of the skin. Enhanced absorption of MMW energy by blood vessels traversing the fat layer may play a primary role in initiating MMW effects on blood cells and vasodilatation of cutaneous blood vessels.

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Comparison of histologic, biochemical, and mechanical properties of murine skin treated with the 1064‐nm and 1320‐nm Nd:YAG lasers

Cited by 35 publications

References 28 publications

Non-ablative collagen remodeling initiated by different laser effects: a comparative study on mouse model

Non-ablative collagen remodeling initiated by different laser effects: a comparative study on mouse model

TREATMENT OF ENLARGED PORES WITH THE QUASI LONG-PULSED VERSUS Q-SWITCHED 1064nm Nd:YAG LASERS: A SPLIT-FACE, COMPARATIVE, CONTROLLED STUDY

Enhanced absorption of millimeter wave energy in murine subcutaneous blood vessels

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