Comparison of histopathologic effects of carnitine and ascorbic acid on reperfusion injury

Akar, Hacı; Saraç, Atilla; Konuralp, Cüneyt; Yıldız, Levent; Kolbakir, F.

doi:10.1016/s1010-7940(01)00615-7

Cited by 29 publications

(19 citation statements)

References 15 publications

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“…These molecules play a key role in tissue damage by affecting the surrounding and distant endothelial cells (24). In the reperfusion period, the molecules formed during the earlier mentioned ischemia, increase the damage in tissues even more (25,26). During the reperfusion period, these toxic molecules are carried to distant organs by blood, where they cause respiratory insuffi ciency, heart dysfunctions and systemic toxic reactions which are life-threatening in organs such as lungs, heart, liver, and kidneys (27-30).…”

Section: Discussionmentioning

confidence: 99%

Iloprost and vitamin C attenuates acute myocardial injury induced by suprarenal aortic ischemia-reperfusion in rabbits

İriz¹,

İriz²,

Take³

et al. 2015

BLL

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OBJECTIVE: The aim of this study was to evaluate antioxidant and cytoprotective effects of iloprost and Vitamin C in a distant organ after abdominal aorta ischemia-reperfusion injury. MATERIAL AND METHODS: Twenty-eight New Zealand rabbits weighing 2,400-2,800 g were used for this study. The rabbits were divided into four equal groups. These groups are control group, sham group, iloprost group, and iloprost + vitamin C group. Suprarenal aorta was occluded with a vascular clamp. Following 30 minutes of ischemia, the vascular clamp was removed. Rabbits in group 3 received 10 ng/kg/min iloprost and those in group 4 received 10 ng/kg/min iloprost and 10 mg/kg vitamin C. At the end of the reperfusion period, the rabbits were sacrifi ced by a high intraperitoneal dose of xylazine + ketamine injection. Myocardial tissue samples were taken for electron microscopic analysis. We evaluated SOD, MDA and catalase in myocardial tissue samples. RESULTS: Iloprost and iloprost + vitamin C groups signifi cantly reduced the oxidative stress markers in tissue samples (p < 0.05) and signifi cantly decreased the myofi brillar injury and mitochondrial morphology changes in the myocardial tissue as shown with electron microscopy (p < 0.05). Myocardial edema was signifi cantly alleviated by iloprost and iloprost + vitamin C administration (p < 0.05). CONCLUSIONS: This study clearly showed that myocardial injury and edema occurred after ischemia-reperfusion of abdominal aorta and that groups administered with iloprost and iloprost + vitamin C showed an attenuation of ischemia-reperfusion injury in distant organs (Tab. 3, Fig. 4, Ref. 30). Text in PDF www.elis.sk.

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Section: Discussionmentioning

confidence: 99%

Iloprost and vitamin C attenuates acute myocardial injury induced by suprarenal aortic ischemia-reperfusion in rabbits

İriz¹,

İriz²,

Take³

et al. 2015

BLL

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“…High-dose vitamin C and total knee replacement membrane integrity causes the release of calcium and phospholipid A 2 as well as formation of polyunsaturated fatty acids and fatty acid radicals. 12 Once oxygenation occurs during reperfusion, fatty acid radicals react with oxygen, triggering lipid peroxidation that increases membrane permeability and stimulates leucocyte chemotaxis. 12 Thus, cells can release oxygenderived free radicals and proteolytic enzymes, 13 and these materials damage the cells containing DNA, proteins and lipids.…”

Section: Discussionmentioning

confidence: 99%

“…12 Once oxygenation occurs during reperfusion, fatty acid radicals react with oxygen, triggering lipid peroxidation that increases membrane permeability and stimulates leucocyte chemotaxis. 12 Thus, cells can release oxygenderived free radicals and proteolytic enzymes, 13 and these materials damage the cells containing DNA, proteins and lipids. Consequently, amino acid modifications including 8-hydroxydeoxyguanosine, carbonyls, methionin sulphoxide and MDA are produced.…”

Section: Discussionmentioning

confidence: 99%

Effect of High-Dose Vitamin C on Oxygen Free Radical Production and Myocardial Enzyme after Tourniquet Ischaemia–Reperfusion Injury during Bilateral Total Knee Replacement

2010

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This study investigated the effects of highdose vitamin C on oxygen free radical production and cardiac enzymes after tourniquet application and ischaemiareperfusion injury during bilateral total knee replacement (TKR) in elderly patients. In the vitamin C (VC) group (VC group, n = 16), during surgery, patients received a priming bolus of 0.06 g/kg vitamin C with 100 ml saline followed by 0.02 g/kg vitamin C mixed with 30 ml saline, intravenously. The control group (n = 16) received no intra-operative vitamin C. In the VC group, malondialdehyde levels were lower, and arterial oxygen tension and mean blood pressure were higher, than in controls after post-operative deflation of both knee tourniquets. Troponin I levels were lower in the VC group than in controls 8 h postoperation.Administering high-dose vitamin C during bilateral TKR could prevent oxygen free radical production and a decline in arterial oxygen tension and mean blood pressure induced by ischaemia-reperfusion injury, thereby protecting the myocardium.

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“…1 Ischemia-reperfusion injury may occur after the re-introduction of oxygenated blood to ischemic tissues, due to released free oxygen radicals and activated neutrophils. 2 After acute inflammatory response, secondary organ dysfunction and finally organ failure might occur, due to free oxygen radicals and leukocyte aggregation.…”

Section: Introductionmentioning

confidence: 99%

Curcumin and dexmedetomidine prevents oxidative stress and renal injury in hind limb ischemia/reperfusion injury in a rat model

et al. 2016

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Curcumin and dexmedetomidine have been shown to have protective effects in ischemia-reperfusion injury on various organs. However, their protective effects on kidney tissue against ischemia-reperfusion injury remain unclear. We aimed to determine whether curcumin or dexmedetomidine prevents renal tissue from injury that was induced by hind limb ischemia-reperfusion in rats. Fifty rats were divided into five groups: sham, control, curcumin (CUR) group (200 mg/kg curcumin, n ¼ 10), dexmedetomidine (DEX) group (25 lg/kg dexmedetomidine, n ¼ 10), and curcumin-dexmedetomidine (CUR-DEX) group (200 mg/kg curcumin and 25 lg/kg dexmedetomidine). Curcumin and dexmedetomidine were administered intraperitoneally immediately after the end of 4 h ischemia, just 5 min before reperfusion. The extremity re-perfused for 2 h and then blood samples were taken and total antioxidant capacity (TAC), total oxidative status (TOS) levels, and oxidative stress index (OSI) were measured, and renal tissue samples were histopathologically examined. The TAC activity levels in blood samples were significantly lower in the control than the other groups (p < 0.01 for all comparisons). The TOS activity levels in blood samples were significantly higher in Control group and than the other groups (p < 0.01 for all comparison). The OSI were found to be significantly increased in the control group compared to others groups (p < 0.001 for all comparisons). Histopathological examination revealed less severe lesions in the sham, CUR, DEX, and CUR-DEX groups, compared with the control group (p < 0.01). Rat hind limb ischemia-reperfusion causes histopathological changes in the kidneys. Curcumin and dexmedetomidine administered intraperitoneally was effective in reducing oxidative stress and renal histopathologic injury in an acute hind limb I/R rat model. ARTICLE HISTORY

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Comparison of histopathologic effects of carnitine and ascorbic acid on reperfusion injury

Abstract: Both sodium ascorbate and carnitine are effective on reducing the reperfusion injury in skeletal muscle. But when we compared these two agents to each other, we found that carnitine seems a little more protective on our experimental model.

Cited by 29 publications

References 15 publications

Iloprost and vitamin C attenuates acute myocardial injury induced by suprarenal aortic ischemia-reperfusion in rabbits

Iloprost and vitamin C attenuates acute myocardial injury induced by suprarenal aortic ischemia-reperfusion in rabbits

Effect of High-Dose Vitamin C on Oxygen Free Radical Production and Myocardial Enzyme after Tourniquet Ischaemia–Reperfusion Injury during Bilateral Total Knee Replacement

Curcumin and dexmedetomidine prevents oxidative stress and renal injury in hind limb ischemia/reperfusion injury in a rat model

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