2019
DOI: 10.1038/s41419-019-1498-0
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of human isogeneic Wharton’s jelly MSCs and iPSC-derived MSCs reveals differentiation-dependent metabolic responses to IFNG stimulation

Abstract: Variability among donors, non-standardized methods for isolation, and characterization contribute to mesenchymal stem/stromal cell (MSC) heterogeneity. Induced pluripotent stem cell (iPSCs)-derived MSCs would circumvent many of current issues and enable large-scale production of standardized cellular therapy. To explore differences between native MSCs (nMSCs) and iPSC-derived MSCs (iMSCs), we developed isogeneic lines from Wharton’s jelly (WJ) from the umbilical cords of two donors (#12 and #13) under xeno-fre… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
12
0

Year Published

2019
2019
2022
2022

Publication Types

Select...
8
2

Relationship

1
9

Authors

Journals

citations
Cited by 16 publications
(13 citation statements)
references
References 60 publications
1
12
0
Order By: Relevance
“…Devito et al also observed senescent cells during iMSC differentiation when using a similar method with single-cell plating and incubation of separated iPSCs in MSC medium containing hPL [24]. Interestingly, they did not observe senescent cells when using a protocol in which iPSCs were first incubated with the TGF-β signaling inhibitor SB-431542 to induce differentiation for 20 days, before separation and selection of iMSCs in MSC medium [24].…”
Section: Discussionmentioning
confidence: 97%
“…Devito et al also observed senescent cells during iMSC differentiation when using a similar method with single-cell plating and incubation of separated iPSCs in MSC medium containing hPL [24]. Interestingly, they did not observe senescent cells when using a protocol in which iPSCs were first incubated with the TGF-β signaling inhibitor SB-431542 to induce differentiation for 20 days, before separation and selection of iMSCs in MSC medium [24].…”
Section: Discussionmentioning
confidence: 97%
“…These cells have characteristics similar to stem cells and can differentiate into a number of different cell types. Metabolomics studies on stem cells could provide insights into their level of differentiation or secretory function 12 , 41 , 42 , give better control of scale-up processing 43 , reveal differences in donor sources 44 , and offer information on their efficacy 45 , 46 . In addition, MSC culture conditions can significantly impact their immunomodulatory potential when transplanted into inflammatory environments 47 .…”
Section: Resultsmentioning
confidence: 99%
“…Fresh umbilical cords (UC) were collected for scientific study from three healthy donors who were informed and consented. Mesenchymal tissue was scraped from Wharton’s jelly after blood vessels were removed (Devito et al 2019 ). After cutting it into pieces, the tissue was centrifuged at 600 g for 10 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%