Comparison of hydrodistillation and ultrasonic solvent extraction for the isolation of volatile compounds from two unifloral honeys of Robinia pseudoacacia L. and Castanea sativa L.

Abstract

A comparative study of ultrasound-assisted extraction (USE) with the mixture pentane:ether (1:2) and hydrodistillation (HD) with the same trapping mixture is presented for the isolation of volatile compounds from two unifloral honeys of Robinia pseudoacacia L. and Castanea sativa L. All HD isolates contained many thermally derived artefacts (especially phenylacetaldehyde with lower percentages of furfural, cis- and trans-linalool oxides and others). USE method gave the most representative profile of all honey volatiles (without artefacts). In addition, USE enabled extraction of low molecular weight semivolatile markers (especially benzoic, vanillic and phenylacetic acids) that were not extracted by HD. In this regard, low percentage of benzoic acid (0.7-7.4%), vanillic acid (0.0-1.6%) and phenylacetic acid (0.5-4.1%) was determined in Rp USE extracts, while Cs USE extracts contained phenylacetic acid (20.2-23.5%) as the major constituent with low percentage of benzoic acid (2.5-5.5%).

Characterization of the volatile profile of unifloral honey from Kashmir Valley of India by using solid-phase microextraction and gas chromatography–mass spectrometry

“…Of the organic acids identified, hexadecanoic acid was present in apple honey (M. domestica) (0.0162 µg/g) and in cherry honey (P. avium) (0.0762 µg/g), acetic acid (0.0519 µg/g) and stearic acid (0.0011 µg/g) only in cherry honey and apple honey, respectively, while there was no organic acid found in saffron honey (C. sativus). These results were in agreement with the studies conducted by Jerkovic et al [11] and Lusic et al [34] which found hexadecanoic acid in the unifloral honeys of black locust (Robinia pseudoacacia L.), chestnut (C. sativa L.) and fir honeydew honey (Abies alba), respectively. Hexadecanoic acid has been reported in apple blossom (M. domestica) as well as in Mahaleb cherry (Prunus mahaleb), thus revealing similarity of both volatile profiles [23,40].…”

Contribution of the Bees and Combs to Honey Volatiles: Blank‐Trial Probe for Chemical Profiling of Honey Biodiversity

“…H 2 O in a 100-ml flask. MgSO 4 (1.5 g) was added, and each sample was extensively vortexed [10] [28]. Different extraction solvents were separately used for USE: 1) a mixture of pentane/Et 2 O 1 :2 (v/v), 2) pentane, and 3) Et 2 O (applied to the samples after sonication with pentane and after removing the pentane extract).…”

“…Only a few compounds seem to be really specific, and many of them can be found in variable concentrations in various honey types. Ultrasonic solvent extraction (USE) is a suitable method to obtain reliable honey fingerprint of more and less volatile (low-and high-molecular) compounds in comparison with other methods such as hydrodistillation or simultaneous distillation -extraction [9] [10].…”

Screening of Volatile Composition of Lavandula hybridaReverchon II Honey Using Headspace Solid‐Phase Microextraction and Ultrasonic Solvent Extraction

“…H 2 O in a 100-ml flask. MgSO 4 (1.5 g) was added, and each sample was extensively vortexed [19] [20]. Different extraction solvents were separately used: a mixture pentane/Et 2 O 1:2 (v/v), pentane, and Et 2 O (applied on the samples after sonication with pentane and after removing the pentane layer).…”