Comparison of Hypervariable Region (HVR) of mecA Gene in Staphylococcus aureus Isolated From Nasal Carriers and Clinical Samples

nia, Neda zeinali; Pourmand, Mohammad Reza; Afrough, Parviz

doi:10.5812/jjm.7686

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Previous studies in Iran have reported a high rate of MRSA infections (9,23). The results of the current study showed an MRSA infection rate of 20.4% in the samples collected from different wards.…”

Section: Discussionsupporting

confidence: 55%

Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran

Havaei

Halaji

Vidović

et al. 2017

Jundishapur J Microbiol

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Background: Staphylococcus aureus is one of the major causes of community-and hospital-acquired infections, with methicillinresistant strains showing the highest rates of morbidity and mortality. In our previous experiment, isolates, which were also used in the present study, were assessed using multilocus sequence typing (MLST). The sequence types (STs) were determined and documented in the corresponding database. Objectives: In the current study, the isolates were subjected to genotyping with coagulase, SCCmec, and agr typing methods. Methods: A total of 54 isolates were evaluated by polymerase chain reaction (PCR) assay for mecA gene, Sccmec typing, and finally PCR-restriction fragment length polymorphism (RFLP) for coagulase (coa) gene using Alul enzyme. MLST of the isolates showed that the majority of methicillin-resistant S. aureus (MRSA) isolates belonged to ST239. Results: Phenotypic and genotypic tests revealed that 21% of the isolates were MRSA. PCR-RFLP test for coa gene showed similar patterns of MRSA isolates. The majority of the isolates were community-acquired and belonged to the Sccmec type IV, whereas the remaining were hospital-acquired and classified as type I (22.2%) and type III (2.2%). Conclusions: Most of the isolates belonged to agr type I, followed by type II and type III. Agar dilution method showed higher sensitivity and specificity, compared to the disk diffusion method. The majority of the isolates were community-acquired and belonged to Sccmec type IV and agr type I, whereas the remaining were hospital-acquired and classified as types I (22.2%), type III (2.2%), and agr type I.

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Section: Discussionsupporting

confidence: 55%

Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran

Havaei

Halaji

Vidović

et al. 2017

Jundishapur J Microbiol

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“…In human medicine, S. aureus nasal carriage has been extensively studied in patients and healthy individuals ( 19 , 20 ). In Iran, resistance and nasal carriage of S. aureus in healthy children ( 21 ) and health care workers ( 22 ) have also been studied. The latter research also concluded that S. aureus could be transmitted from health care workers to hospitalized patients.…”

Section: Introductionmentioning

confidence: 99%

Nasal Carriage of Staphylococcus aureus: Frequency and Antibiotic Resistance in Healthy Ruminants

Rahimi¹,

Saei²,

Ahmadi³

2015

Jundishapur J Microbiol

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Background:Staphylococcus aureus is a significant pathogen that can colonize the nares of different animals, causing a wide range of infections in various hosts.Objectives:We intended to determine the prevalence of S. aureus in the nasal cavity of healthy ruminants and also to investigate the presence of antibiotic resistance genes.Materials and Methods:In the present study, healthy cattle (n = 79), sheep (n = 78) and goats (n = 44) were screened for nasal carriage of S. aureus by the Polymerase Chain Reaction (PCR). Staphylococcus aureus isolates were further assessed for the presence of blaZ (encoding penicillin resistance), mecA (encoding methicillin resistance), tetK and tetM (encoding tetracycline resistance), and ermA and ermC (encoding macrolide-lincosamide-streptogramin B resistance) genes.Results:The proportion of S. aureus-positive nasal swabs from cattle, sheep and goats were four (5.06%), 11 (14.1%) and 11 isolates (25%), respectively. The blaZ gene was detected in 20 out of 26 S. aureus isolates (76.9%), including four cattle (100%), nine sheep (81.8%) and seven goats (63.6%). Two of the four cattle isolates possessing the blaZ gene also had the tetK gene. Of the nine sheep isolates harboring the blaZ gene, one possessed the mecA and tetK genes together. Of the seven goat isolates with blaZ gene, one harbored the tetM gene. None of the S. aureus isolates were positive for the ermA and ermC genes.Conclusions:In contrast to cattle, S. aureus is frequently present in the nose of sheep and goats, which may represent the primary reservoir of S. aureus in small ruminant flocks. This study also showed that nasal isolates of S. aureus from healthy ruminants might be a potential reservoir of antimicrobial-resistance.

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Comparison of Hypervariable Region (HVR) of mecA Gene in Staphylococcus aureus Isolated From Nasal Carriers and Clinical Samples

Cited by 2 publications

References 12 publications

Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran

Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran

Nasal Carriage of Staphylococcus aureus: Frequency and Antibiotic Resistance in Healthy Ruminants

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