Comparison of <i>Macrophomina phaseolina</i> inoculation techniques for screening sunflower and soybean germplasm in a controlled environment

Talapov, Talap; Yuceer, S.; Dedecan, Olcay; DEMİREL, Özge; Chen, Can

doi:10.1080/07060661.2021.1937321

Cited by 2 publications

(3 citation statements)

References 25 publications

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“…The ITP method was inefficient since it did not allow for differentiation of the genotypes while presenting the lowest values in both evaluations. According to Talapov et al (2021), the ITP method promotes long incubation periods and low repeatability, generating slow disease development. The PGS method allowed only symptoms of the disease to appear in some genotypes, indicating that it should not be used for all tested genotypes.…”

Section: Ta B L E 2 Analysis Of Variance Resultsmentioning

confidence: 99%

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Methods for inoculation and evaluation of resistance to Macrophomina phaseolina in soybean genotypes

Bizari,

Panizzi,

Pedroso Val

et al. 2024

Journal of Phytopathology

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Charcoal rot, caused by Macrophomina phaseolina, is a disease of economic significance in soybean production. The evaluation of this disease is difficult due to the lack of reliable and efficient methods for testing soybean genotypes. This work aimed at investigating different inoculation methods for Macrophomina phaseolina in soybean genotypes to determine the degree of resistance and their use in genetic improvement programs. Infected sorghum seeds, pregerminated seed in culture medium, and infected toothpick test were the methods tested. Colonization by the fungus, determined as a colony‐forming unit (CFU), and disease development based on root and stem severity (RSS) were evaluated. There was a good correlation between CFU and RSS (0.87), with the infected sorghum seed method being the most efficient means of inoculating. The results show that PI594302 was the most resistant line regarding the two studied parameters and would be a source of resistance to charcoal rot in breeding programs.

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Section: Ta B L E 2 Analysis Of Variance Resultsmentioning

confidence: 99%

“…According to Talapov et al. (2021), the ITP method promotes long incubation periods and low repeatability, generating slow disease development. The PGS method allowed only symptoms of the disease to appear in some genotypes, indicating that it should not be used for all tested genotypes.…”

Section: Discussionmentioning

confidence: 99%

Methods for inoculation and evaluation of resistance to Macrophomina phaseolina in soybean genotypes

Bizari,

Panizzi,

Pedroso Val

et al. 2024

Journal of Phytopathology

View full text Add to dashboard Cite

show abstract

“…phaseolina can be occur due to different hosts (Talapov et al, 2021). Although non-inoculated plants had the narrowest interval between the most resistant and the most susceptible inbred lines, this method showed variation among inbred lines.…”

Section: Discussionmentioning

confidence: 96%

Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina

et al. 2022

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The fast adaptation to different growing conditions of a fungus Macrophomina phaseolina, led to its becoming one of the sunflower (Helianthus annuus L.) disease causal agents in regions with a temperate climate.Methods currently used to determine sunflower resistance require laborious manual inoculation and confirmation of pathogen appearance, due to the late stage of testing. The paper proposes a cut-stem method for inoculating sunflower plants in the controlled conditions and the possibility of early-stage disease evaluation. A set of 15 sunflower inbred lines was inoculated using M. phaselolina isolate in the growth chamber and the obtained data were analysed using Cut-stem Disease Severity (CSDS) and compared with disease severity obtained from field experiments using traditional inoculation methods (toothpick, Unwounded Stem Base Inoculation (USBI) and non-inoculated plants).The results showed that, based on CSDS, inbred lines infected with the cut-stem inoculation method significantly differed regarding resistance to M. phaseolina. None of the inbred lines exhibited complete resistance but three lines could be proposed as a source of resistance to this pathogen. Ranking of inbred lines which was based on resistance to M.phaseolina was similar in all inoculation methods and in non-inoculated plants. There were highly significant correlations between the values obtained from growth chamber experiment and disease severity scores from field evaluations. Thus, the obtained results indicate that the cut-stem method could potentially complement field testing methods and be valuable tool in sunflower breeding for resistance to М. phaseolina.

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Comparison of Macrophomina phaseolina inoculation techniques for screening sunflower and soybean germplasm in a controlled environment

Cited by 2 publications

References 25 publications

Methods for inoculation and evaluation of resistance to Macrophomina phaseolina in soybean genotypes

Methods for inoculation and evaluation of resistance to Macrophomina phaseolina in soybean genotypes

Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina

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