Comparison of identification methods of vancomycin-resistant enterococci in MALDI-TOF MS, Vitek 2 and API 20 STREP

Chabros, Łukasz; Kuthan, Robert; Sawicka-Grzelak, A.; Młynarczyk, G.

doi:10.5604/08606196.1152292

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…[24, 26] However, our SBEAVM method was different from previous studies in several aspects. First, to increase the specificity of the BEAV method, two carbapenems were added to the broth to better inhibit growth of gram negative bacteria, in addition to the bile in BEAV which served as an inhibitor.…”

Section: Discussionmentioning

confidence: 99%

“…Second, our comparator was ChromID VRE method, not conventional phenotypic identification,[ 24 ] Vitek 2 or API 20 STREP. [ 26 ] Third, the database we used for identification was Vitek v2.0, which is approved by the US Federal and Drug Administration for clinical use, while the database used in the previous study was for research use only. [ 24 ] In conclusion, our study proposed a better solution to VRE screening in the real world.…”

Section: Discussionmentioning

confidence: 99%

“…[ 22 , 25 ] The application of MALDI-TOF MS to identify bacteria isolated from BEAV medium may increase specificity of the VRE screening process and decrease the turnaround time, but this possibility has been rarely investigated in previous studies. [ 24 , 26 ]…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk

Huang¹,

Lee²,

Lee³

et al. 2017

PLoS ONE

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BackgroundBile esculin azide with vancomycin (BEAV) medium is a sensitive, but slightly less specific method for vancomycin-resistant enterococci (VRE) screening. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid method for identification of clinical pathogens. This study aimed to assess the performance of a novel combination screening test for VRE, using BEAV broth combined with MALDI-TOF MS.Materials and methodsClinical specimens were collected from patients at risk of VRE carriage, and tested by the novel combination method, using selective BEAV broth culture method followed by MALDI-TOF MS identification (SBEAVM). The reference method used for comparison was the ChromID VRE agar method.ResultsA total of 135 specimens were collected from 78 patients, and 63 specimens tested positive for VRE positive using the ChromID VRE method (positive rate 46.7%). The sensitivity, specificity, positive predictive value, and negative predictive value of SBEAVM method after an incubation period of 28 hours were 93.7%, 90.3%, 89.4%, and 94.2%, respectively. The SBEAVM method when compared to the ChromID VRE method had a shorter turnaround time (29 vs. 48–72 hours) and lower laboratory cost ($2.11 vs. $3.23 per test).ConclusionsThis study demonstrates that SBEAVM is a rapid, inexpensive, and accurate method for use in VRE screening.

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Section: Discussionmentioning

confidence: 99%