Comparison of immune responses to different foot-and-mouth disease genetically engineered vaccines in guinea pigs

Yao, Qingxia; Qian, Ping; Huang, Qing-Guo; Cao, Yi; Chen, Huanchun

doi:10.1016/j.jviromet.2007.08.027

Cited by 14 publications

(5 citation statements)

References 39 publications

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“…Due to the reproducibility of the FMDV response, guinea pigs have been used extensively to produce antiserum, which has been used to develop sensitive diagnostic and serotyping assays (Ferris, 1988). Guinea pigs have also been used extensively for FMDV vaccine efficacy trials (Cartwright et al , 1982; Guo et al , 2005; Yao et al , 2008). It is noteworthy that natural FMDV isolates need to be adapted to the guinea pig by serial injection in the footpad (Aramburu, 1949; Knudsen et al , 1979).…”

Section: Introductionmentioning

confidence: 99%

Laboratory animal models to study foot-and-mouth disease: a review with emphasis on natural and vaccine-induced immunity

Habiela

Seago

Pérez-Martín

et al. 2014

Journal of General Virology

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Laboratory animal models have provided valuable insight into foot-and-mouth disease virus (FMDV) pathogenesis in epidemiologically important target species. While not perfect, these models have delivered an accelerated time frame to characterize the immune responses in natural hosts and a platform to evaluate therapeutics and vaccine candidates at a reduced cost. Further expansion of these models in mice has allowed access to genetic mutations not available for target species, providing a powerful and versatile experimental system to interrogate the immune response to FMDV and to target more expensive studies in natural hosts. The purpose of this review is to describe commonly used FMDV infection models in laboratory animals and to cite examples of when these models have failed or successfully provided insight relevant for target species, with an emphasis on natural and vaccine-induced immunity. IntroductionFoot-and-mouth disease virus (FMDV: family Picornaviridae; genus Aphthovirus) is known to naturally infect a wide variety of cloven-hoofed domesticated and wild animal species, causing an acute disease characterized by vesicular lesions of the tongue, snout, buccal cavity, feet and teats (Grubman & Baxt, 2004). Despite causing extensive lesions, the cycle of infection in the individual animal is short, and foot-and-mouth disease (FMD) usually resolves without the need for treatment and is seldom lethal in adults (Arzt et al., 2011b). However, the highly contagious nature, wide dissemination and significant economic impact of FMD have made it one of the most feared livestock diseases and a major research focus for more than a century. Progress towards the development of effective tools for FMD control has been hampered by several factors including the cost and logistics of largeanimal experimentation in specialized high-containment facilities, incomplete knowledge of the host's immune systems and lack of immunological reagents compared to biomedical rodent species and humans. These factors delayed the production of vaccines on an industrial scale and this major research goal was subsequently only achieved in the 1950s (Lombard et al., 2007). In a review, Brown (2003) highlighted that this milestone could not have been achieved without certain significant advances in our knowledge of FMD. The first significant advance was the demonstration by Loeffler & Frosch (1897) that the disease was caused by a virus and the second was the establishment of FMD laboratory animal models, including the guinea-pig model (Waldman & Pape, 1920) followed by the suckling mouse model (Skinner, 1951). Although not without their flaws, these FMD laboratory animal models have helped elucidate several mechanisms of FMD pathogenesis, which would have been difficult to achieve directly in target species. These models have provided an accelerated time frame at significantly reduced costs to develop and test vaccine candidates and continue to be a useful tool for interrogating FMDV immune responses. However, we now know that porcine and ru...

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Section: Introductionmentioning

confidence: 99%

Laboratory animal models to study foot-and-mouth disease: a review with emphasis on natural and vaccine-induced immunity

Habiela

Seago

Pérez-Martín

et al. 2014

Journal of General Virology

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“…After centrifugation at 4 • C, 4500 rpm for 15 min, the serum samples were collected and stored at −80 • C. Serum of each group was heat-inactivated at 56 • C for 30 min and diluted at a rate of 1:20 in PBS. The serum neutralization titers were measured by CyHV-2 and GiCB cells according to the previous report [25]. Briefly, a serial 1:2 dilution of the serum was mixed with equal volume of CyHV-2 containing 50% TCID 50 mL −1 and incubated at 37 • C for 1 h (the dilution range is between 1:2 to 1:40).…”

Section: Serum Antibody Neutralization Tests and Biochemistry Index Amentioning

confidence: 99%

The Combination of Molecular Adjuvant CCL35.2 and DNA Vaccine Significantly Enhances the Immune Protection of Carassius auratus gibelio against CyHV-2 Infection

Huo

Fan

et al. 2020

Vaccines

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Cyprinid herpesvirus 2 (CyHV-2) infection results in huge economic losses in gibel carp (Carassius auratus gibelio) industry. In this study, we first constructed recombinant plasmids pcORF25 and pcCCL35.2 as DNA vaccine and molecular adjuvant against CyHV-2, respectively, and confirmed that both recombinant plasmids could be effectively expressed in vitro and in vivo. Then, the vaccination and infection experiments (n = 50) were set as seven groups. The survival rate (70%) in ORF25/CCL35.2 group was highest. The highest specific antibody levels were found in ORF25/CCL35.2 group in major immune tissues by qRT-PCR, and confirmed in serum by ELISA assay, antibody neutralization titer, and serum incubation-infection experiments. Three crucial innate immune indices, namely C3 content, lysozyme, and total superoxide dismutase (TSOD) activities, were highest in ORF25/CCL35.2 group in serum. pcORF25/pcCCL35.2 can effectively up-regulate mRNA expressions of some important immune genes (IL-1β, IL-2, IFN-γ2, and viperin), and significantly suppress CyHV-2 replication in head kidney and spleen tissues. The minimal tissue lesions can be seen in ORF25/CCL35.2 group in gill, spleen, and trunk kidney tissues by histopathological examination. The results indicated that the combination of DNA vaccine pcORF25 and molecular adjuvant pcCCL35.2 is an effective method against CyHV-2 infection, suggesting a feasible strategy for the control of fish viral diseases.

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“…Some researchers had an attempt to exploit recombinant vaccinia virus [32] fowlpox virus [33] and pseudorabies virus [34] expressing FMDV structural protein, non-structure protein or both of them, but swine and guinea pigs inoculated with multiple doses were partially protected because of their low level of expression. However, FMD vaccines are required to protect animals from FMDV challenge by a single dose of vaccine in the epidemic period.…”

Section: Live Vector Vaccinementioning

confidence: 99%

Research in advance for FMD Novel Vaccines

Zhang

Chen

et al. 2011

Virol J

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Foot-and-Mouth Disease (FMD), as a major global animal disease, affects millions of animals worldwide and remains the main sanitary barrier to the international and national trade of animals and animal products. Inactivated vaccination is the most effective measure for prevention of FMD at present, but fail to induce long-term protection and content new requires for production of FMD vaccines. As a number of Researchers hope to obtain satisfactory novel vaccines by new bio-technology, novel vaccines have been studied for more than thirty years. Here reviews the latest research progress of new vaccines, summarizes some importance and raises several suggestions for the future of FMD vaccine.

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Comparison of immune responses to different foot-and-mouth disease genetically engineered vaccines in guinea pigs

Cited by 14 publications

References 39 publications

Laboratory animal models to study foot-and-mouth disease: a review with emphasis on natural and vaccine-induced immunity

Laboratory animal models to study foot-and-mouth disease: a review with emphasis on natural and vaccine-induced immunity

The Combination of Molecular Adjuvant CCL35.2 and DNA Vaccine Significantly Enhances the Immune Protection of Carassius auratus gibelio against CyHV-2 Infection

Research in advance for FMD Novel Vaccines

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