Comparison of immunomodulatory effects of placenta mesenchymal stem cells with bone marrow and adipose mesenchymal stem cells

Lee, Jung Min; Jung, Jieun; Lee, Hyunjung; Jeong, Su Jin; Cho, Kyung Jin; Hwang, Seong-Gyu; Kim, Gi Jin

doi:10.1016/j.intimp.2012.03.024

Cited by 171 publications

(134 citation statements)

References 36 publications

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“…As depicted in Table 1, and as reported by us and others, HLA-G is expressed by WJ-MSC [16,47] and BM-MSC [48]. HLA-G has a key role in tolerance induction of the mother's immune system towards the semi-allogeneic foetus during pregnancy; it acts together with other class Ib MHC molecules (such as HLA-E and HLA-F) and trophoblast-secreted factors as EPF (early pregnancy factor) [77,78].…”

Section: Recent Data On the Expression Of Relevant Immunomodulatory Msupporting

confidence: 76%

“…One of the suggested effects due to MSC-expressed HLA-G is the stimulation of regulatory T cells (CD4+CD25+FoxP3+ Tregs). These T lymphocytes have been characterized as key suppressors of effector responses to alloantigens [48,85]. Interestingly, the action mechanisms through which HLA-G may exert this function are almost two and distinct: the membrane-bound isoform is involved in immune modulation mediated by direct intercellular contact, while the secreted isoform (known as HLA-G5) is shedded from the cellular membrane.…”

Section: Recent Data On the Expression Of Relevant Immunomodulatory Mmentioning

confidence: 99%

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Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Rocca¹,

Corrao

Iacono

et al. 2012

TOTERMJ

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Mesenchymal (stromal) stem cells (MSC) are a broad class of stromal populations which are able to differentiate towards mature cell types, and do express molecules involved in immune modulation, tolerance induction and inflammation dampening. MSC can be virtually isolated from each adult organ, as well as from foetus-associated perinatal tissues. In particular, Wharton's jelly-derived MSC (WJ-MSC) bear all of these key properties, together with their ease of sourcing and lack of ethical issues.Cellular therapy is a key technique in regenerative medicine approaches, in particular for the treatment of diseases in which physiological processes of cellular repopulation are blocked by the underlying pathological conditions. Recent data enlightened the ability of administered cells to act also in a repopulation-independent fashion in target organs, since their peculiar immunomodulatory and anti-inflammatory features may favor organ self-repair by reactivating local progenitors by both cell-mediated or paracrine mechanisms. Translating classical regenerative medicine to "reparative medicine" or "support medicine" should represent a further therapeutic strategy independent from the differentiation capacity of MSC populations.Recent data further highlighted that WJ-MSC outperform BM-MSC (which are now being applied clinically) both in terms of immune modulation and lack of tumorigenesis (or tumor-promoting activities) in vivo. Starting from these premises, this paper analyzes the recent data on the biology of WJ-MSC, considering the role of both naïve and differentiated cells in immune modulation. In particular, the role of tolerance promoting pathways via non-classical B7 costimulators or class Ib MHC molecules are examined. In addition, we also analyzed the interconnections with other mechanicistic pathways (as those driven by matrix degrading metalloproteinases) in immune modulation. Our observations strongly support the notion that WJ-MSC may constitute a new tool in regenerative and reparative medicine applications.

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Section: Recent Data On the Expression Of Relevant Immunomodulatory Msupporting

confidence: 76%

Section: Recent Data On the Expression Of Relevant Immunomodulatory Mmentioning

confidence: 99%

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Rocca¹,

Corrao

Iacono

et al. 2012

TOTERMJ

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“…In utero intervention allows clinicians to treat disease quickly after its onset and prevent debilitating symptoms before they ever occur [3,4] . Mesenchymal stromal cell (MSC) therapy can augment existing in utero surgical techniques, and treatment with MSCs has been shown to promote wound healing [5,6] , protect damaged tissues [7][8][9][10][11] , and modulate the immune system [12][13][14][15] . Cells obtained via methods such as chorionic villus sampling (CVS) allow for the potential development of cell therapies that are autologous (derived from the patient's cells) to the fetus [16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Early gestation chorionic villi-derived stromal cells for fetal tissue engineering

Lankford¹

2015

WJSC

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AIM:To investigate the potential for early gestation placenta-derived mesenchymal stromal cells (PMSCs) for fetal tissue engineering. METHODS:PMSCs were isolated from early gestation chorionic villus tissue by explant culture. Chorionic villus sampling (CVS)-size tissue samples (mean = 35.93 mg) were used to test the feasibility of obtaining large cell numbers from CVS within a clinically relevant timeframe. We characterized PMSCs isolated from 6 donor placentas by flow cytometry immunophenotyping, multipotency assays, and through immunofluorescent staining. Protein secretion from PMSCs was examined using two cytokine array assays capable of probing for over 70 factors in total. Delivery vehicle compatibility of PMSCs was determined using three common scaffold systems: fibrin glue, collagen hydrogel, and biodegradable nanofibrous scaffolds made from a combination of polylactic acid (PLA) and poly(lactic-co-glycolic acid) (PLGA). Viral transduction of PMSCs was performed using a Luciferase-GFPcontaining lentiviral vector and efficiency of transduction was tested by fluorescent microscopy and flow cytometry analysis. RESULTS:We determined that an average of 2.09 × 10 6 (SD ± 8.59 × 10 5 ) PMSCs could be obtained from CVS-size tissue samples within 30 d (mean = 27 d, SD ± 2.28), indicating that therapeutic numbers of cells can be rapidly expanded from very limited masses of tissue. Immunophenotyping by flow cytometry demonstrated that PMSCs were positive for MSC markers CD105, CD90, CD73, CD44, and CD29, and were negative for hematopoietic and endothelial markers CD45, CD34, and CD31. PMSCs displayed trilineage differentiation capability, and were found to express developmental transcription factors Sox10 and Sox17 as well as neuralrelated structural proteins NFM, Nestin, and S100β. Cytokine arrays revealed a robust and extensive profile of PMSC-secreted cytokines and growth factors, and detected 34 factors with spot density values exceeding 10 3 . Detected factors had widely diverse functions that include modulation of angiogenesis and immune response, cell chemotaxis, cell proliferation, blood vessel maturation and homeostasis, modulation of insulin-like growth factor activity, neuroprotection, extracellular matrix degradation and even blood coagulation. Importantly, PMSCs were also determined to be compatible with both Core tip: In this study we characterize mesenchymal stromal cells derived from early gestation human placenta chorionic villi (PMSCs) for the purpose of fetal tissue engineering. We examine cell expansion in early passages from chorionic villus sampling-size tissue samples, as well as PMSC surface marker expression, multipotency, intracellular protein expression, protein secretion, and compatibility with delivery vehicles and tracking methods often used for in vivo experimentation. We show that early gestation PMSCs are excellent candidates for future tissue engineering studies, particularly as it applies to in utero therapy for congenital anomalies.

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“…Our results showing that all tested MSCs did not express constitutively one of the main mediators of immunosuppression, HLA-G5, were unexpected, since previously published data evidenced the expression of HLA-G5 in MSCs from different tissue sources [31]. As for the molecules related to the paracrine effects of MSCs, a marked expression of IL-6 and COX2 mRNA was demonstrated for the AT-MSCs and DP-MSCs, a low level of IDO-1 transcript only in AT-MSCs, whereas AT-MSCs, PBMSCs and UC-MSCs displayed similar low expression levels for the TGF-β mRNA.…”

Section: Discussionmentioning

confidence: 41%

Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton’s jelly

Trivanović

Mojsilović²,

Ilić³

et al. 2013

cejoi

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Comparison of immunomodulatory effects of placenta mesenchymal stem cells with bone marrow and adipose mesenchymal stem cells

Cited by 171 publications

References 36 publications

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Novel Immunomodulatory Markers Expressed by Human WJ-MSC: an Updated Review in Regenerative and Reparative Medicine

Early gestation chorionic villi-derived stromal cells for fetal tissue engineering

Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton’s jelly

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