Comparison Of Immunonephelometric And Immunoturbidimetric Methods for Measuring Beta 2-Microglobulin: Fit for Purpose in Routine Clinical Laboratories

Ariyurek, Sedefgul Yuzbasioglu

doi:10.4172/2161-1009.1000119

Cited by 1 publication

(1 citation statement)

References 24 publications

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“…bTP and b2M measurements were carried out using nephelometric and turbidimetric techniques, respectively. Alternative methods are available for both proteins, 32,33 and variation between different assays may therefore limit applicability of our equations. Additionally, although the precision of both assays seems robust in non-uremic serum, we cannot exclude the potential interference of the assay by toxins related to advanced uremia or the dialysis procedure;…”

Section: Discussionmentioning

confidence: 99%

Predicting residual kidney function in hemodialysis patients using serum β-trace protein and β2-microglobulin

Wong

Sridharan

Berdeprado

et al. 2016

Kidney International

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Residual kidney function (RKF) contributes significant solute clearance in hemodialysis patients. Kidney Diseases Outcomes Quality Initiative (KDOQI) guidelines suggest that hemodialysis dose can be safely reduced in those with residual urea clearance (KRU) of 2 ml/min/1.73 m(2) or more. However, serial measurement of RKF is cumbersome and requires regular interdialytic urine collections. Simpler methods for assessing RKF are needed. β-trace protein (βTP) and β2-microglobulin (β2M) have been proposed as alternative markers of RKF. We derived predictive equations to estimate glomerular filtration rate (GFR) and KRU based on serum βTP and β2M from 191 hemodialysis patients based on standard measurements of KRU and GFR (mean of urea and creatinine clearances) using interdialytic urine collections. These modeled equations were tested in a separate validation cohort of 40 patients. A prediction equation for GFR that includes both βTP and β2M provided a better estimate than either alone and contained the terms 1/βTP, 1/β2M, 1/serum creatinine, and a factor for gender. The equation for KRU contained the terms 1/βTP, 1/β2M, and a factor for ethnicity. Mean bias between predicted and measured GFR was 0.63 ml/min and 0.50 ml/min for KRU. There was substantial agreement between predicted and measured KRU at a cut-off level of 2 ml/min/1.73 m(2). Thus, equations involving βTP and β2M provide reasonable estimates of RKF and could potentially be used to identify those with KRU of 2 ml/min/1.73 m(2) or more to follow the KDOQI incremental hemodialysis algorithm.

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Section: Discussionmentioning

confidence: 99%