Comparison of immunoregulatory effects of polysaccharides from three natural herbs and cellular uptake in dendritic cells

Zhu, Ning; Lv, Xiaochen; Wang, Yangyun; Li, Jingling; Liu, Yongming; Lu, Wenfei; Yang, Lijing; Zhao, Jian; Wang, Fujun; Zhang, Leshuai W.

doi:10.1016/j.ijbiomac.2016.09.064

Cited by 60 publications

(25 citation statements)

References 45 publications

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“…Polysaccharide treatment not only activates macrophages, lymphocytes, lymphoid factorsactivated killer cells, toxic T cells, and dendritic cells but also promotes the production of cytokines, activates the complement system, and accelerates the production of antibodies [24,25]. After treatment with BCG-PSN, GPS, and SFPS (Table 2), NKT-like cells produced more IFN-γ and TNF-α, which are both important for the killing of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

In vitro Assessment of Polysaccharides Immunoregulatory Effects on CD3+CD56+ NKT-like Cells

Huang

Lian-yang

et al. 2021

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Aims: Polysaccharides have shown efficacy in a broad range of applications, especially in immune regulation. However, there are only few effective methods available to evaluate and compare the immunoregulatory properties of the different polysaccharides in vitro. To screen stringently and extend the fields of application of polysaccharide-based treatments, a novel in vitro method, based on the use of cord blood-derived CD3+CD56+ NKT-like cells, was developed in this study. Methodology: Four injectable polysaccharides used in clinics, including Lentinan (LNT), Bacillus Calmette-Guerin polysaccharide nucleic acids (BCG-PSN), ginseng polysaccharides (GPS), and Shengfu polysaccharides (SFPS), which all might benefit cancer therapy, were further validated by testing their effect on cell proliferation and cytotoxicity in vitro. This new evaluation system could discriminate the effects of the four polysaccharides. Results: Cells treated for 4 days with either of the four polysaccharides were fewer than untreated cells. Upon 7-day treatments, plant- or bacteria-derived BCG-PSN, GPS, and SFPS induced higher level of IFN-, proportion of cells with a CD3+CD56+ phenotype, and cytolytic activity toward A549 cells, compared to controls. However, fungus-derived LNT yielded lower IFN-, proportion of CD3+CD56+ cells, and cytolytic activity than control conditions.Additionally, all four polysaccharides, in decreasing order GPS, SFPS, BCG-PSN, and LNT, induced significantly higher concentrations of TNF-α compared to control, with respectively. Conclusion:Cord blood-derived CD3+CD56+ NKT-like cells may represent a powerful tool to screen and evaluate the immunoregulatory potential of biological or chemical compounds for clinical developments and applications.

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Section: Discussionmentioning

confidence: 99%

In vitro Assessment of Polysaccharides Immunoregulatory Effects on CD3+CD56+ NKT-like Cells

Huang

Lian-yang

et al. 2021

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“…[ 21 ] Polysaccharides isolated from Astragalus membranaceus are able to stimulate dendritic cells maturation through increasing expression of CD40, MHC II, CD80, and CD86. [ 22 ] Isoliquiritigenin and liquiritigenin which are isolated from Astragalus membranaceus can significantly inhibit the production of IL-6 and IL-12 p40 stimulated by lipopolysaccharide in bone marrow derived dendritic cells. Isoliquiritigenin also has a moderate inhibition on LPS-induced TNF-α production.…”

Section: Discussionmentioning

confidence: 99%

The therapeutic effect of modified Huangqi Guizhi Wuwu Tang for multiple myeloma

Tian

Huang²

2017

Medicine

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“…Zhu and colleagues compared PDPs extracted from three herbs at the same concentration in vitro namely, Astragalus membranaceus, G. lucidum and Radix ophiopogonis, all of which were previously reported to positively influence DC maturation. For the parameters measured they concluded that G. lucidum exhibited the strongest activity while R. ophiopogonis exhibited the weakest one (Zhu et al 2016). Previously, when comparing highly purified polysaccharide extracts from A. membranaceus and Codonopsis pilosulae, Chang et al reported that in culture the former induced significantly a higher expression of CD40 while the latter induced higher expression of CD80 and CD86.…”

Section: Comparisons and Combinationsmentioning

confidence: 97%

“…Endocytosis of Ganoderma lucidum polysaccharides results in the upregulation of CD40, CD80, CD86 and MHCII. These maturation markers were significantly depressed when sodium azide (an endocytosis inhibitor) was co-cultured with polysaccharide treated DCs, indicating that endocytosis is critical for DC maturation (Zhu et al 2016).…”

Section: Exogenous Maturation Stimulimentioning

confidence: 99%

Plant-derived polysaccharides activate dendritic cell-based anti-cancer immunity

et al. 2018

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Today, cancers pose a major public health burden. Although a myriad of cancer treatments are available, only a few have achieved clinical efficacy. This is partly attributed to cancers capability to evade host immunity by converting dendritic cells (DCs) from potent stimulators to negative modulators of immunity. Dendritic cell-based immunotherapy attempts to resolve this problem by manipulating the functional characteristics of DCs. Plant-derived polysaccharides (PDPs) can stimulate the maturation of DCs conferring on them the capacity to present internalised tumorigenic antigens to naïve T cells and subsequently priming T cells to eliminate tumours. PDPs have been used as immune modulators and later as anti-cancer agents by Traditional Chinese Medicine practitioners for centuries. They are abundant in nature and form a large group of heterogeneous though structurally related macromolecules that exhibit diverse immunological properties. They can induce antigen pulsed DCs to acquire functional characteristics in vitro which can subsequently be re-introduced into cancer patients. They can also be used as adjuvants in DC-based vaccines or independently for their intrinsic anti-tumour activities. Clinically, some in vitro generated DCs have been shown to be both safe and immunogenic although their clinical application is limited in part by unsatisfactory functional maturation as well as impaired migration to draining lymph nodes where T cells reside. We review the relative potencies of individual PDPs to induce both phenotypic and functional maturation in DCs, their relative abilities to activate anti-cancer immunity, the possible mechanisms by which they act and also the challenges surrounding their clinical application.

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Comparison of immunoregulatory effects of polysaccharides from three natural herbs and cellular uptake in dendritic cells

Cited by 60 publications

References 45 publications

In vitro Assessment of Polysaccharides Immunoregulatory Effects on CD3+CD56+ NKT-like Cells

In vitro Assessment of Polysaccharides Immunoregulatory Effects on CD3+CD56+ NKT-like Cells

The therapeutic effect of modified Huangqi Guizhi Wuwu Tang for multiple myeloma

Plant-derived polysaccharides activate dendritic cell-based anti-cancer immunity

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