Comparison of in situ ruminal straw fiber degradation and bacterial community between buffalo and Holstein fed with high-roughage diet

Pu, Xuan Xuan; Zhang, Xiu Min; Li, Qiu Shuang; Wang, Rong; Zhang, Min; Zhang, Shizhe; Lin, Bo; Tan, Bin; Tan, Zhi Liang; Wang, Min

doi:10.3389/fmicb.2022.1079056

Cited by 8 publications

(8 citation statements)

References 44 publications

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“…The ED of NDF and ADF in GS and AS were similar, but the b value of NDF in GS was 73.15%, which was significantly higher than that of AS (49.22%), and the degradation rate of ADF in GS (55.45%) was also significantly higher than that of AS (50.84%) at 72 h. This indicates that NDF in GS can be degraded slowly and continuously by rumen. These results are consistent with previous studies, which reported a negative correlation between rumen degradation and fiber content [ 34 , 35 ].…”

Section: Discussionsupporting

confidence: 94%

“…Therefore, the number of Prevotella in GS and AS decreased in different degrees in the later stage of degradation, which was consistent with the experimental results of Vahidi et al [ 54 ]. The relative abundance of Prevotella in GS was higher than that in AS, which may be due to the low fiber content in GS, and Prevotella tended to mobilize readily available nutrients (such as soluble sugars and proteins) [ 34 ]. Treponema and Ruminococcus are hydrolytic and acid-producing bacteria [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

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Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Gu,

Liu,

Jiang

et al. 2024

Animals

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The purpose of this study was to study the chemical composition, rumen degradation characteristics, surface attached microbial community and cellulase activity of garlic skin (GS) and Artemisia argyi stalk (AS), in order to explain their feeding value. Four 14-month-old healthy Min Dong male goats with permanent rumen fistula were selected as experimental animals. The rumen degradation characteristics of GS and AS were determined by using the nylon bag method, and the bacterial composition, cellulase activity and their relationship on the surface of the two groups were analyzed with high-throughput sequencing of 16S rRNA gene. The results showed that in GS and AS, the effective degradation rate (ED) values of dry matter (DM) were 42.53% and 37.12%, the ED values of crude protein (CP) were 37.19% and 43.38%, the ED values of neutral detergent fiber (NDF) were 36.83% and 36.23%, and the ED values of acid detergent fiber (ADF) were 33.81% and 34.77%. During rumen degradation, the richness and evenness of bacteria attached to the AS surface were higher. At the phylum level, Bacteroidetes and Firmicutes were always the main rumen bacteria in the two groups. At the genus level, fiber-degrading bacteria such as Prevotella, Treponema, and Ruminococcus showed higher levels in GS (p < 0.05). Compared with GS, the activity of β-glucosidase (BG enzyme), endo-β-1,4-glucanase (C1 enzyme), exo-β-1,4-glucanase (Cx enzyme) and neutral xylanase (NEX enzyme) attached to AS surface showed a higher trend. Correlation analysis showed that the relative abundance of Succinivibrio and Rikenellaceae_RC9_gut_group was positively correlated with the rumen degradability of nutrients in GS, and the relative abundance of Christensenellaceae R-7_group, Succinivibrio and Ruminococcus was positively correlated with the rumen degradability of nutrients in AS. The conclusion of this study shows that AS has more potential to become ruminant roughage than GS. In addition, this study also revealed the relationship between cellulase activity and bacteria, which provided new information for us to better analyze the effects of GS and AS on the rumen of ruminants and provided an important theoretical basis for the development and utilization of agricultural by-products.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Gu,

Liu,

Jiang

et al. 2024

Animals

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“…2C), consistent with the distribution patterns seen in prokaryotic communities within the ruminant GIT 6 . Notably, GIT regions had greater variance (31.2%) S9), which aligns with rumen being the largest compartment in the ruminant GIT and hosting the highest bacterial populations 38 . Such distinct viral communities among GIT regions could be attributable to multiple factors, such as substrate availabilities and microbial metabolism 6 .…”

Section: Ruminant Viral Communities Are Diverse and Primarily Driven ...mentioning

confidence: 65%

Spatial heterogeneity of viral communities across the gastrointestinal tracts of ruminants

Wang

2024

Preprint

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The gastrointestinal tract (GIT) microbiome of ruminants significantly influences their nutrition metabolism and health. Current understanding is extensive for bacterial and archaeal communities, but limited for viral communities within the GIT. To address this, we created the Ruminant Gastrointestinal Virome Catalogue (RGVC), which includes 43,981 non-redundant viral Operational Taxonomic Units (vOTUs), with 89.3% newly identified, derived from 370 samples across 10 GIT regions in seven ruminant species. The composition of viral communities is mainly influenced by the GIT regions rather than by the ruminant species. We identified 4,603 putative prokaryotic hosts across 31 bacterial and three archaeal classes for 5,954 viruses, along with significant variations across GIT regions and a strong correlation between hosts and their associated viruses. Lysogeny, constituting 45.6% of survival strategies, was more prevalent than the lytic cycle (4.08%), and the abundances of these viruses varied regionally. The lysogenic viruses encoded 1,805 auxiliary metabolic genes (AMGs) that play key roles in carbohydrates, amino acids, and other metabolisms in their hosts. The variation in AMG abundance across regions highlights the distinct viral communities and the varied virus-host interactions within the GIT. Overall, this study offers a comprehensive view of the spatial heterogeneity of viral communities in the ruminant GIT and indicates that this diversity is driven by the interaction of lysogenic viruses with their prokaryotic hosts through AMGs. These findings set the stage for future research into the ecological and nutritional impacts of the ruminant virome, with the potential to uncover novel roles and mechanisms in various GIT regions.

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“…Distinct forages demonstrate varied dry matter (DM) degradation rates upon rumen entry, with the microbial composition attached to particles differing across feeds [9]. However, Comprehensive knowledge about rumen bacterial diversity, especially concerning feeds rich in cellulose and hemicellulose, remains limited [10][11][12]. The genus Prevotella, notably dominant, plays a significant role in enzyme families associated with fiber degradation [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Impact of Nutrient Composition and Tissue Structure on Rumen Microbiome Dynamics in Roughage Degradation

Ye,

Li,

et al. 2024

Preprint

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Background Ruminant animals, such as goats, depend on rumen microbial communities to degrade fibrous nutrients from roughages, facilitating their growth and development. This research delved into the dynamic shifts in surface-attached rumen microbes found in representative roughages (rice straw, bamboo shoot sheet, and alfalfa) and examined their degradation characteristics. Four 14-month-old Min Dong goats with rumen fistulas were used for the experiment, and the roughages were assessed at seven intervals (4 h, 12 h, 24 h, 36 h, 48 h, and 72 h). Using the 16S rRNA and metagenomics sequencing techniques to reveal the microbiome composition and their functions. Results Prevotella and Treponema were pinpointed as pivotal genera in roughage degradation. The nutritional composition and tissue structure of roughages affected microbial attachment, causing variations in nutrient degradation rates and the overall degradation process. Microbes related to dry matter (DM) and crude protein (CP) degradation were abundant in the early fermentation stages (4-12h) while decreased as time progressed. In contrast, microbes of fiber degradation increased gradually after 24-hour. Dominant in the goat rumen, Prevotella and Treponema are integral to roughage degradation, attributed to their multifaceted functional traits. Furthermore, the surface-attached microbes in the three roughages produced BG (β-Glucosidase), C1 (Endo-β-1,4-glucanase), Cx (Exo-β-1,4-glucanase), and NEX (Neutral xylanase) enzymes. The activity of these enzymes and their correlation with GHs (Glycoside Hydrolases) functional genes increased with the fiber content of the roughages. Conclusions These insights advance our understanding of microbial roles in ruminant nutrition and digestion. The interaction between microbial communities and rumen fermentation is pivotal to understanding the collaborative gene encoding by goat rumen microbiota being critical for fiber degradation.

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Comparison of in situ ruminal straw fiber degradation and bacterial community between buffalo and Holstein fed with high-roughage diet

Cited by 8 publications

References 44 publications

Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Analysis of Rumen Degradation Characteristics, Attached Microbial Community, and Cellulase Activity Changes of Garlic Skin and Artemisia argyi Stalk

Spatial heterogeneity of viral communities across the gastrointestinal tracts of ruminants

Deciphering the Impact of Nutrient Composition and Tissue Structure on Rumen Microbiome Dynamics in Roughage Degradation

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