Comparison of in vitro and in vivo Dog Dentin Permeability

Pashley, David H.; Kehl, Thomas; Pashley, Edna L.; Palmer, P. H.

doi:10.1177/00220345810600031801

Cited by 44 publications

(25 citation statements)

References 14 publications

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“…This behavior is analogous to that observed in our previous report, that the permeability of dog dentin was similar when studied in vivo or in vitro, in the same teeth (Pashley et al, 1981a). These observations suggest that dentin is relatively stable over time.…”

Section: Resultssupporting

confidence: 90%

Comparison of in vivo vs. in vitro Bonding of Composite Resin to the Dentin of Canine Teeth

et al. 1988

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Dogs were utilized in a study to compare the bond strengths of dentin bonding agents made to dentin in vivo and then again in vitro in the same teeth 30 min, one day, one week, and one month post-extraction. No statistically significant differences were observed between bonds made in vivo and those made in vitro at any time period. Contamination of the dentin surfaces with blood or saliva lowered the bond strengths, but these could be restored to control values by re-surfacing of the dentin with a bur.

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Section: Resultssupporting

confidence: 90%

Comparison of in vivo vs. in vitro Bonding of Composite Resin to the Dentin of Canine Teeth

et al. 1988

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“…2.5 ml was chosen as the collection volume, to allow four 0.5-ml samples from each tooth to be tested. The various data on the diffusion of known substances across dentin (Pashley et al, 1977(Pashley et al, , 1978b(Pashley et al, , 1981Hume, 1984a) indicated that one day was a reasonable initial collection time in which to expect peak availability of applied chemicals at the pulpal surface.…”

Section: Resultsmentioning

confidence: 99%

A New Technique for Screening Chemical Toxicity to the Pulp from Dental Restorative Materials and Procedures

Hume

1985

J Dent Res

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An in vitro test system is described which allows for quick and relatively inexpensive examination of the potential for chemical toxicity to the pulp of materials and procedures used in the restoration of single teeth. The test system consisted of two sequential steps. First, a restorative procedure was carried out on a freshly-extracted human tooth crown, to the pulpal surface of which had been attached a chamber filled with sterile tissue-culture medium. The preparation was kept at 37 degrees C. The culture medium was removed at day one and replaced with fresh medium, which was removed at day 3. In the second step, we used a standard tissue-culture toxicity assessment technique to examine both culture medium samples for the presence of chemical toxins. In use, this system gave results which correlated well with the known clinical potential for pulpal toxicity of various dental materials and techniques. For example, zinc oxide-eugenol used as temporary filling or base had no apparent potential for toxicity. Sealing a cotton pellet containing phenol into a cavity was of high apparent potential toxicity. Acrylic resin as intracoronal or extracoronal fillings showed potential for toxicity; this potential was decreased by lining with calcium hydroxide cement. Composite resin placed onto etched dentin had apparent toxic potential, but had less such potential when placed onto unetched dentin. The technique had some advantages over previously described in vitro toxicity test for restorative materials, because it included a step requiring diffusion of potential toxins into and through human dentin, and because it allowed for examination of variations in technique which mimic clinical behavior, and of materials used in sequence or in combination.

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“…He demonstrated that a smear layer existing on polished surfaces, reduces water movement through the dentin tubules. [28] Surface etching resulted in lower contact angles on enamel E3(E) (Figure 2a and 3E3(E)). On dentin the contact angle is decreased only for water D3(E) (Figure 2b and 3D3(E)).…”

Section: Wettabilitymentioning

confidence: 92%

Modification of Enamel and Dentin Surfaces by Non‐Thermal Atmospheric Plasma

Lehmann

André

Schindler

et al. 2013

Plasma Processes & Polymers

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Tooth material surface chemical and micromechanical properties are important for success and sustainability in restorative dentistry. Modification by cold atmospheric plasma jet treatment may help to improve presently existing shortcomings as a part of future restorative therapies. The present study has been focused on changes of surface properties of tooth substances by this approach. Polished and etched enamel and dentin slices from the vestibular face of bovine incisors are used. A plasma jet modifies these surfaces at a distance of 2 mm and with a scan velocity of 5.5 mm Á s À1. The treatment parameters are: helium process gas flow 3.525 slm, plasma excitation by 2.45 GHz with an average pulsed microwave power of 2 W, single pulse power of 250 W, pulse width 5 ms. The surface modifications are subjected to laser scanning microscope roughness-measurement, contact angle measurements, X-ray photoelectron spectroscopy (XPS) analysis and morphological visualization by scanning electron microscopy (SEM). Plasma treatment causes changes of surface roughness and morphological alterations on dentin, while no such changes are measureable on etched dentin and polished and etched enamel surfaces. Plasma causes contact angle reductions for both, water and ethylene glycol. XPS analyses show significant carbon reduction on enamel surfaces after plasma treatment. Admixture of 30% of hydrogen peroxide or deionized water, respectively, as precursors in the plasma jet processing gas flow generates measureable amounts of CÀO-bindings at the surfaces assigned to hydrophilic groups. Surface modifications by plasma treatment might enable interesting new options for dental procedures in all areas of interaction between tooth substance and restorative materials.

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Comparison of in vitro and in vivo Dog Dentin Permeability

Cited by 44 publications

References 14 publications

Comparison of in vivo vs. in vitro Bonding of Composite Resin to the Dentin of Canine Teeth

Comparison of in vivo vs. in vitro Bonding of Composite Resin to the Dentin of Canine Teeth

A New Technique for Screening Chemical Toxicity to the Pulp from Dental Restorative Materials and Procedures

Modification of Enamel and Dentin Surfaces by Non‐Thermal Atmospheric Plasma

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