Comparison of in vitro Antifungal Activity Methods Using Extract of Chitinase-producing Aeromonas sp. BHC02

Abstract

Biological control to prevent fungal plant diseases offers and alternative approach to faciliate sustainable agriculture. Since inhibition of chitinolytic fungal cell walls synthesis is a target for antifungal agents, chitinases are one of the biocontrol agents. This study, it was aimed to investigate isolating a new bacterium from uvial soil as a chitinase source and the antifungal activity of the characterized chitinase. During planning the in vitro antifungal activity, three common methods were preferred and compared. The bacterium with the highest chitinase activity was identi ed as Aeromanas caviae by 16S rRNA sequence analysis. Following the determination of the optimum enzyme production time, the enzyme was partially puri ed, and the physicochemical parameters of the enzyme were investigated. It was determined that the partially puri ed chitinase showed antifungal activity against Alternaria alternata, Fusarium solani, Botrytis cinerea, Penicillium sp. This study also conclude that the results of the antifungal activities depend on the method used. And all fungal chitins cannot be degraded with a chitinase. Depending on the variety of chitin, some fungi can be more resistant. In this context, it is necessary to conduct a detailed study on the chitins in the cell wall of the fungi.

Bioformulados para mantener la viabilidad de rizobacterias y su aplicación en Theobroma cacao L. CCN-51

“…Ecuador, 2020. M a n u s c r i t o a c e p t a d o son productoras de ácidos orgánicos, control de hongos fitopatógenos por actividad de la enzima quitinasa, disminuir el estrés y estimular el crecimiento de plantas hospederas mediante la producción de fitohormonas y enzimas ACC desaminasa, y precipitación de metales pesados por la producción de biopelículas (Cadirci & Yilmaz, 2023;Haque et al, 2023;Nagrale et al, 2023;Wang et al, 2023). Esta particularidad mejora el estado fisiológico de las especies vegetales, ya sea directa o indirecta, e incrementa la productividad de cultivos agrícolas y minimiza la dependencia de los agroquímicos (Mishra et al, 2020).…”