Comparison of in vitro photodynamic antimicrobial activity of protoporphyrin IX between endoscopic white light and newly developed narrowband endoscopic light against Helicobacter pylori 26695

Choi, SungSook; Lee, HaeKyung; Chae, Hiun-Suk

doi:10.1016/j.jphotobiol.2012.08.015

Cited by 10 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various authors concluded that although DNA damage occurred, it might not be the prime cause of bacterial cell death. In previous studies by Caminos and Choi, slightly degraded DNA bands could only be detected after a relative long time irradiation, although the bacterial strains were effectively inactivated [2,8]. The damage might be repaired by various DNA repairing systems.…”

Section: Photodynamic Effect On Genomic Dnamentioning

confidence: 83%

Mechanistic Aspects of the Photodynamic Inactivation of Vancomycin-Resistant Enterococci Mediated by 5-Aminolevulinic Acid and 5-Aminolevulinic Acid Methyl Ester

et al. 2014

View full text Add to dashboard Cite

Vancomycin-resistant Enterococci (VRE) is a serious concern for public health. Serious infections with VRE have very limited effective antimicrobial therapy, and alternative treatment approaches are highly desirable. One promising approach might be the photodynamic antimicrobial chemotherapy. In the present study, we investigated the photodynamic inactivation (PDI) of two VRE strains mediated by 5-aminolevulinic acid (5-ALA) and its derivative 5-ALA methyl ester (MAL). The photodynamic damages to bacteria on the level of genomic DNA, the leakage of cell components, and the changes of membrane structure were investigated. After treated with 10 mM 5-ALA and irradiated by the 633 ± 10 nm LED for 60 min, 5.37 and 5.22 log10 reductions in bacterial survival were achieved for the clinical isolate of VRE and E. faecalis (ATCC 51299), respectively. After treated with 10 mM MAL and irradiated by the LED for 60 min, 5.02 and 4.91 log10 reductions in bacterial survival were observed for the two VRE strains, respectively. In addition, the photocleavage on genomic DNA and the rapid release of intracellular biopolymers were detected in PDI-treated bacteria. The intensely denatured cytoplasm and the aggregated ribosomes were also found in PDI-treated bacteria by transmission electron microscopy. Although 5-ALA and MAL-mediated PDI could induce the photocleavage on genomic DNA, the PDI of the two VRE strains might be predominantly attributed to the envelope injury, the intracellular biopolymers leakage, and the cytoplasm denature.

show abstract

Section: Photodynamic Effect On Genomic Dnamentioning

confidence: 83%

Mechanistic Aspects of the Photodynamic Inactivation of Vancomycin-Resistant Enterococci Mediated by 5-Aminolevulinic Acid and 5-Aminolevulinic Acid Methyl Ester

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Gastrointestinal endoscopes have been developed that emit wavelengths that activate porphyrins in patients to detect cancer, and similar strategies have been interrogated for their ability to treat gastrointestinal infections (38,39). Osteomyelitis and contamination of orthopedic devices are some of the most common invasive bacterial infections, and PDT strategies to combat these infections are being developed (40)(41)(42)(43)(44)(45)(46).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial photosensitization through activation of coproporphyrinogen oxidase

Surdel

Horvath

Lojek

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Gram-positive bacteria cause the majority of skin and soft tissue infections (SSTIs), resulting in the most common reason for clinic visits in the United States. Recently, it was discovered that Gram-positive pathogens use a unique heme biosynthesis pathway, which implicates this pathway as a target for development of antibacterial therapies. We report here the identification of a small-molecule activator of coproporphyrinogen oxidase (CgoX) from Gram-positive bacteria, an enzyme essential for heme biosynthesis. Activation of CgoX induces accumulation of coproporphyrin III and leads to photosensitization of Gram-positive pathogens. In combination with light, CgoX activation reduces bacterial burden in murine models of SSTI. Thus, small-molecule activation of CgoX represents an effective strategy for the development of light-based antimicrobial therapies.

show abstract

“…As a precursor of heme, PPIX has been used as a representative PS for PDT. It is maximally activated when exposed to light at a wavelength of 410 nm, and it also shows variable absorption at wavelengths of 540 nm, 580 nm, and 635 nm 34,35 . H. pylori only expresses the uvrA gene to repair light‐induced DNA damage 36 .…”

Section: Mechanism Of Photodynamic Therapy For Helicobacter Pylorimentioning

confidence: 99%

“…It is maximally activated when exposed to light at a wavelength of 410 nm, and it also shows variable absorption at wavelengths of 540 nm, 580 nm, and 635 nm. 34,35 H. pylori only expresses the uvrA gene to repair light-induced DNA damage. 36 However, the explosive production of a large amount of ROS leads to significant damage to DNA, which makes it impossible to repair the damage.…”

mentioning

confidence: 99%

Research progress in photodynamic therapy for Helicobacter pylori infection

Luo,

Liu,

Zhang

et al. 2024

Helicobacter

View full text Add to dashboard Cite

Helicobacter pylori (H. pylori) is a pathogenic microorganism that colonizes the human gastric mucosa and can lead to various gastric disorders, including gastritis, gastric ulcers, and gastric cancer. However, the increasing prevalence of antibiotic resistance in H. pylori has prompted the search for alternative treatment options. Photodynamic therapy has emerged as a potential alternative therapy, thus offering the advantage of avoiding some of the side effects associated with antibiotics and effectively targeting drug‐resistant strains. In the postantibiotic era, photodynamic therapy (PDT) has shown promise as a novel treatment for H. pylori infection. This review focused on elucidating the mechanism of photodynamic therapy in the treatment of H. pylori. Additionally, we present an overview of the current research on photodynamic therapy by examining both standalone photodynamic therapy and combination therapies for H. pylori infection treatment. Furthermore, the safety profile of photodynamic therapy was also evaluated. Finally, we discuss the challenges and prospects associated with this innovative technology, with an aim to provide new insights and methodologies for the treatment of H. pylori infection.

show abstract

Comparison of in vitro photodynamic antimicrobial activity of protoporphyrin IX between endoscopic white light and newly developed narrowband endoscopic light against Helicobacter pylori 26695

Cited by 10 publications

References 23 publications

Mechanistic Aspects of the Photodynamic Inactivation of Vancomycin-Resistant Enterococci Mediated by 5-Aminolevulinic Acid and 5-Aminolevulinic Acid Methyl Ester

Mechanistic Aspects of the Photodynamic Inactivation of Vancomycin-Resistant Enterococci Mediated by 5-Aminolevulinic Acid and 5-Aminolevulinic Acid Methyl Ester

Antibacterial photosensitization through activation of coproporphyrinogen oxidase

Research progress in photodynamic therapy for Helicobacter pylori infection

Contact Info

Product

Resources

About