Comparison of in Vivo and in Vitro Reporter Gene Assays for Short-Term Screening of Estrogenic Activity

Legler, Juliette; Zeinstra, Laura; Schuitemaker, Femke; Lanser, Peter H.; Bogerd, Jan; Brouwer, Abraham; Vethaak, A.D.; Voogt, P. de; Murk, A.; Burg, Bart van der

doi:10.1021/es010323a

Cited by 185 publications

(147 citation statements)

References 34 publications

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“…This finding is different from data published by Legler et al (2002) who found that all three of the zf ERs, including ERba (ERg in their report) were responsive. The pattern of ER inducibility was also different between bass and zf.…”

Section: Differential Activation Of Lmb Er Subtypes By E 2 and Npcontrasting

confidence: 99%

“…Recently, three ER isotypes have been shown to exist in fish as reported by us and others (Hawkins et al 2000, Legler et al 2002, Sabo-Attwood et al 2004, Filby & Tyler 2005. The three ERs, originally termed ERa, ERb, and ERg, were recently renamed ERa, ERbb, and ERba respectively, to comply with official zebrafish (zf) nomenclature (Hawkins & Thomas 2004, Filby & Tyler 2005.…”

Section: Introductionmentioning

confidence: 92%

“…These include the induction of estrogenresponsive genes in males such as vitellogenins (vtgs) and choriogenins that are involved in normal female reproduction (Allen et al 1999, Arukwe et al 2001, Kannan et al 2003, impaired gonadal development (Lin & Janz 2006), altered steroidogenesis (Harris et al 2001), and intersex (Gray et al 1997, Metcalfe et al 2001, Schwaiger et al 2002, Seki et al 2003. These conditions have been induced in fish in controlled laboratory studies and have also been seen in wild fish found in the vicinity of sewage outflows (Allen et al 1999, Jobling et al 2002, Legler et al 2002, Pettersson et al 2005 where the main contaminants include NP.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, one study showed that NP weakly activated the three zfERs in human embryonic kidney 293 (HEK293) cells (Legler et al 2002). This result was similar but not identical to E 2 , suggesting that NP may bind and activate the ER isotypes differently than the endogenous ligand.…”

Section: Introductionmentioning

confidence: 99%

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Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Sabo‐Attwood

Blum

Kroll

et al. 2007

Journal of Molecular Endocrinology

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The estrogen receptor (ER) signaling cascade is a vulnerable target of exposure to environmental xenoestrogens, like nonylphenol (NP), which are causally associated with impaired health status. However, the impact of xenoestrogens on the individual receptor isotypes (a, ba, and bb) is not well understood. The goal of these studies was to determine the impact of NP on largemouth bass (Micropterus salmoides) ER isotype expression and activity. Here, we show that hepatic expression levels of three receptors are not equivalent in male largemouth bass exposed to NP by injection. Transcript levels of the ERa subtype were predominantly induced in concert with vitellogenin similarly to fish exposed to 17b-estradiol (E 2 ) as measured by quantitative real-time PCR. NP also induced circulating plasma levels of estrogen, which may contribute to overall activation of the ERs. To measure the activation of each receptor isotype by E 2 and NP, we employed reporter assays using an estrogen response element (ERE)-luciferase construct. Results from these studies show that ERa had the greatest activity following exposure to E 2 and NP. This activity was inhibited by the antagonists ICI 182 780 and ZM 189 154. Furthermore, both bb and ba subtypes depressed ERa activation, suggesting that the cellular composition of receptor isotypes may contribute to the overall actions of estrogen and estrogenic contaminants via the receptors. Results from these studies collectively reveal the differential response of fish ER isotypes in response to xenoestrogens.

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Section: Differential Activation Of Lmb Er Subtypes By E 2 and Npcontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Sabo‐Attwood

Blum

Kroll

et al. 2007

Journal of Molecular Endocrinology

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“…They are excreted by humans and livestock and discharged in the environment with effluents of sewage treatment plants (Kolpin et al 2002;Legler et al 2002;Snyder et al 2003) or with agricultural run off (Lange et al 2002;Johnson et al 2006;Matthiessen et al 2006). Only at certain specific locations, estrogenic activities were partly or fully caused by high concentrations of alkylphenols (Sheahan et al 2002;Fenet et al 2003;Cespedes et al 2004;Quiros et al 2005).…”

Section: Endocrine Disrupting Compoundsmentioning

confidence: 99%

Emerging contaminants in surface waters and their relevance for the production of drinking water in Europe

Houtman

2010

Journal of Integrative Environmental Sciences

206

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An increasing part of drinking water in Europe is prepared from surface water. At the same time, a growing number of emerging contaminants is being discovered in surface water. This review provides an overview of classes of emerging contaminants nowadays detected in the aquatic environment that are of relevance for drinking water production. These comprise e.g. endocrine disrupting compounds, such as hormones and compounds with hormone-like properties, pharmaceuticals, illicit and non-controlled drugs, sweeteners, personal care products, complexing agents, nanoparticles, perfluorinated compounds, flame retardants, pesticides, and fuel additives. The individual compounds are observed in concentrations that are generally considered too low to cause acute effects. Nevertheless, health effects due to long-term exposure to a mixture of low concentrations of all kinds of emerging contaminants cannot be excluded with current knowledge. Moreover, contamination of drinking water with man-made substances is considered unwanted in principle. The precautionary principle is used to motivate that prevention of emission of emerging contaminants into the environment is the preferred approach to safeguard sustainable drinking water production. In the mean time, extensive monitoring of the sources and development and application of advanced treatment techniques are used to prepare safe drinking water.

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Zebrafish: An Animal Model for Toxicological Studies

2003

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Zebrafish (Danio rerio) has been extensively studied and well described for environmental toxicity studies. Molecular biology and genetics have recently been used to elucidate the underlying mechanisms of toxicity in zebrafish and to predict effects in mammals. The versatile zebrafish is now incorporated in many areas of toxicological programs for assessing human risk and for preclinical drug discovery and screening.

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Comparison of in Vivo and in Vitro Reporter Gene Assays for Short-Term Screening of Estrogenic Activity

Cited by 185 publications

References 34 publications

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Emerging contaminants in surface waters and their relevance for the production of drinking water in Europe

Zebrafish: An Animal Model for Toxicological Studies

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