Comparison of Induced Sputum and Bronchoalveolar Lavage in Lung Transplant Recipients

Mamessier, Émilie; Milhe, F.; Badier, M; Thomas, Philippe; Magnan, A.; Reynaud‐Gaubert, Martine

doi:10.1016/j.healun.2005.12.009

Cited by 18 publications

(22 citation statements)

References 46 publications

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“…This part of my research continued previous works in the field of chronic lung rejection, having demonstrated the compartmentalisation of immune response in transplanted lungs [2] and the importance of T-regulatory (Treg)-cell activation in stable chronic rejection [3].…”

Section: My Research As Part Of My Working Group/ Research Teamsupporting

confidence: 70%

Dendritic cells commit T-cells to a tolerant phenotype in tolerant lung transplant recipients

Botturi¹,

Lacoeuille²,

Thomas³

et al. 2008

Eur Respir Rev

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WINNING ABSTRACT: Immune characteristics distinguishing tolerant lung transplant recipients (LTR) from patients with Bronchiolitis obliterans syndrome (BOS) are largely unknown. We compared the effect of dendritic cells (DC) on T-cell activation in healthy LTR (non BOS) and in BOS patients.30 Non BOS and 14 BOS recipients were studied. Mature dendritic cells were derived from blood monocytes and co-cultured with autologous T cells at various ratios. T cell CD69, CD25, CD28, ICOS and CTLA-4 expression and IL-4, IL-13, IFN-c, and IL-10 production were assessed by flow cytometry. Dendritic cell expression of surface markers and indoleamine 2,3 dioxygenase was also studied. Experiments were repeated in presence of P. aeruginosa or anti-CTLA-4 antibodies.In dendritic cell/LT co-cultures, T cell CD69, CD28 and ICOS decreased in non BOS (p,0.03). By contrast, CD4 In contrast with BOS recipients, dendritic cells from non BOS induce a tolerant T cell phenotype, by using CD80/CD86-CTLA4 axis.

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Section: My Research As Part Of My Working Group/ Research Teamsupporting

confidence: 70%

Dendritic cells commit T-cells to a tolerant phenotype in tolerant lung transplant recipients

Botturi¹,

Lacoeuille²,

Thomas³

et al. 2008

Eur Respir Rev

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“…The correlation was not a constant finding in older patients and adults (three out of four studies) [27,[33][34][35] (table E2).…”

Section: Convergent Validitymentioning

confidence: 99%

“…The correlation between inflammatory markers obtained via BAL and other sampling methods is variable. Two studies showed no correlation between BAL and induced sputum [6,34], while one study showed excellent agreement between IL-8 recovered from BALF and spontaneously expectorated sputum (r50.967, p50.007) [37]. In contrast to induced sputum, which originates from the airways, BAL samples the bronchial or terminal airways and air spaces (alveoli), according to the fractions analysed (the latter aliquots more likely representing the alveolar region).…”

Section: Convergent Validitymentioning

confidence: 99%

Clinimetric properties of bronchoalveolar lavage inflammatory markers in cystic fibrosis

et al. 2013

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The Standardisation Committee of the European Cystic Fibrosis Society Clinical TrialNetwork has undertaken the evaluation of clinical end-points for therapeutic interventions regarding their use in multicentre clinical trials in cystic fibrosis (CF). This review of biomarkers in bronchoalveolar lavage (BAL) is part of the group's work.The aims of this project were: 1) to review the literature on reliability, validity and responsiveness of BAL in patients with CF; 2) to gain consensus of the group on the feasibility of BAL; and 3) to gain consensus on answers to key questions regarding the promotion of BAL to surrogate end-point status.Assessment of BAL inflammatory markers in the literature indicates that their reliability, validity and responsiveness are adequate for clinical trials. After discussion of the practical characteristics it was concluded that BAL has an attractive validity profile, albeit with limited feasibility. It is particularly applicable to multicentre trials in preschool children with CF and early or mild lung disease. This is the first article to collate the literature in this manner. This provides a rationale to support the use of BAL in early clinical trials in preschool children with CF. @ERSpublications Review of the literature provides rationale to support the use of BAL in well-designed early-phase clinical trials in CF

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Section: Sputum Processingmentioning

confidence: 99%

“…Acute rejection (AR), also a T cell-mediated process, is strongly linked to OB [67]. The T cell activation in blood, IS and BALF during AR, stable OB and evolving OB have recently been investigated [67]. Stable and evolving OB were characterized by a Treg, Th1 and Th2 activation, but compared to evolving OB, Treg and Th2 cells predominated in stable OB.…”

Section: Sputum Processingmentioning

confidence: 99%

Induced Sputum in Interstitial Lung Diseases: Novel Insights in the Diagnosis, Evaluation and Research

Economidou¹,

Samara²,

Αντωνίου³

et al. 2008

Respiration

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The search for noninvasive procedures of retrieving cells and soluble material from the lung has gained momentum over the past few years. Induced sputum (IS) by inhalation of hypertonic saline solution is a noninvasive technique used to collect cellular and soluble material from lung airways. During the past decade, this method has been widely used to assess airway inflammation in asthma and chronic obstructive disease, since it produces reliable results and compares favorably to other invasive techniques, such as biopsy and bronchoalveolar lavage fluid. However, recent attention has been paid to its efficacy in the evaluation of interstitial lung diseases. Recent research in this area clearly showed that IS analysis could give extensive information regarding the inflammation in pulmonary sarcoidosis, such as the lymphocytic cell count, the CD4+/CD8+ ratio and the Th1 immunologic response. The CD4+/CD8+ ratio recovered from lymphocytes from IS is as useful as the same value retrieved from examination of lymphocytes recovered from bronchoalveolar lavage fluid for clinical use. The above findings suggest that integrating IS procedure in the diagnosis, evaluation, follow-up and research in patients with pulmonary sarcoidosis is necessary. Besides sarcoidosis, the review of the current literature in other interstitial lung diseases showed that IS could provide us with useful information regarding inflammatory molecules, but cannot fully replace more invasive techniques. This review analyzes the applications of IS in the assessment of fibrotic and granulomatous diseases such as sarcoidosis and extrinsic allergic alveolitis, idiopathic pulmonary fibrosis, connective tissue disorders, occupational lung diseases and other systemic diseases.

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Comparison of Induced Sputum and Bronchoalveolar Lavage in Lung Transplant Recipients

Cited by 18 publications

References 46 publications

Dendritic cells commit T-cells to a tolerant phenotype in tolerant lung transplant recipients

Dendritic cells commit T-cells to a tolerant phenotype in tolerant lung transplant recipients

Clinimetric properties of bronchoalveolar lavage inflammatory markers in cystic fibrosis

Induced Sputum in Interstitial Lung Diseases: Novel Insights in the Diagnosis, Evaluation and Research

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