Comparison of infrapatellar and subcutaneous adipose tissue stromal vascular fraction and stromal/stem cells in osteoarthritic subjects

Abstract

Since inflammatory mechanisms have been postulated to link obesity to osteoarthritis, the current study evaluated the ratio of immune cells to multipotent stromal cells within the infrapatellar fat pad (IPFP) and subcutaneous adipose tissue (SQ) of the knee; each depot has potential as a source of regenerative cells. The immunophenotypes of stromal vascular fraction (SVF) and adipose‐derived stem cells (ASCs) of the IPFP and SQ were determined in tissues from osteoarthritic subjects (n = 7) undergoing total knee replacement. Based on a subset of surface antigens, the immunophenotype of ASCs from SQ of OA subjects was not significantly different from that of relatively healthy and leaner subjects undergoing elective liposuction surgery. Flow‐cytometry comparison of SVF cell populations in the IPFP of OA subjects resembled those within the subject's own matched SQ, with the exception of the endothelial marker CD31+, which was significantly greater in cells from SQ. In the OA subjects, lower numbers of capillary‐like structures and higher numbers of stromal and alkaline phosphatase colony‐forming units in the IPFP vs SQ were consistent with this finding; however, ASCs from both depots in OA subjects exhibited comparable adipogenic and osteogenic differentiation potential. Thus, the IPFP contains an ASC and immune cell population similar to that of donor‐matched SQ, making it an alternative ASC source for tissue regeneration. Further studies will be needed to determine whether IPFP immune cell infiltrates play an aetiological role in osteoarthritis equivalent to that shown in diabetes associated with obesity. Copyright © 2012 John Wiley & Sons, Ltd.

Influence on Chondrogenesis of Human Osteoarthritic Chondrocytes in Co-Culture with Donor-Matched Mesenchymal Stem Cells from Infrapatellar Fat Pad and Subcutaneous Adipose Tissue

“…Adipose-derived stem cells (ASCs) have been successfully used in several in vivo studies for cartilage generation (14)(15)(16). Thus, when developing treatments for knee chondral lesions, infrapatellar fat pad and knee subcutaneous adipose tissue, that are easily accessible during orthopaedic surgery, can be considered appealing sources for MSCs harvesting (17)(18)(19)(20)(21)(22).…”

Clinical immunology Comparison of phenotype, chondrogenic and osteogenic potential of rheumatoid mesenchymal stem cells derived from articular and subcutaneous adipose tissue - the role of adipocytokines

“…5). Interestingly, AAT-ASCs and ScAT-ASCs from osteoarthritis (OA) subjects were reported to exhibit similar osteogenic potential [18]. Reassuming, it seems that ASCs derived from rheumatoid articular adipose tissue have stronger differentiation potential despite localization in the site of inflammation.…”

Comparison of Stromal/Stem Cells Isolated from Human Omental and Subcutaneous Adipose Depots: Differentiation and Immunophenotypic Characterization

“…Omental ASC were expanded to P1 before their immunophenotype was analyzed. The detailed protocols used for flow cytometry testing have been published in our previous articles [Yu et al, 2010;Carvalho et al, 2011;Pires de Carvalho et al, 2014].…”

“…While the majority of tissue engineering studies using human stromal/stem cells have employed subcutaneous ASC , direct comparison of infrapatellar-and subcutaneous-derived ASC from individual donors found that the two populations did not display significant differences based on differentiation or immunophenotype; the only exception was the endothelial cell-associated antigen, CD31, which was more abundant in subcutaneous ASC [Pires de Carvalho et al, 2014]. Likewise, comparisons between ASC derived from Bichat's or the buccal fat pad and subcutaneous adipose depots of human subjects or pigs have determined that both ASC populations exhibit similarities based on the immunophenotype as well as adipogenic and osteogenic differentiation abilities [Farre-Guasch et al, 2010;Niada et al, 2013;Kishimoto et al, 2014].…”

“…Both ASC and BMSC share a similar differentiation and immunophenotypic profile; however, a unit volume of adipose tissue yields nearly two to three orders of magnitude more ASC at the initial passage as a similar volume of bone marrow Pachon-Pena et al, 2011]. Consequently, there has been a growing interest in the regenerative medical potential of ASC from multiple adipose depots [Wickham et al, 2003;Baglioni et al, 2009;Villaret et al, 2010;De Girolamo et al, 2013;Pires de Carvalho et al, 2014;Russo et al, 2014]. To address this question, we have compared the differentiation and immunophenotypic characteristics between ASC derived from omental adipose depots of subjects undergoing elective bariatric surgery and ASC from subcutaneous adipose depots of subjects undergoing elective plastic surgery.…”