Comparison of inhibitory potency of three different curcuminoid pigments on nitric oxide and tumor necrosis factor production of rat primary microglia induced by lipopolysaccharide

Zhang, Li Jia; Wu, Chun Fu; Meng, Xue; Yuan, Dan; Cai, Xu; Wang, Qi Long; Yang, Jing

doi:10.1016/j.neulet.2008.09.067

Cited by 60 publications

(41 citation statements)

References 30 publications

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“…Our present study reports that DMC can modulate in vitro model of intestinal inflammation by downregulation of iNOS induction at both the transcriptional and translational levels, leading to a decrease in NO production. As DMC can down-regulate iNOS/NO, our results are consistent with the previous reports on the inhibition of iNOS at mRNA and protein levels by DMC in LPS-activated rat primary microglial cells (Zhang et al, 2008) and RAW 264.7 macrophage cells (Guo et al, 2008;Kaewkroek et al, 2010). DMC may have the direct effect in inhibition of NO production via iNOS pathway as NO-scavenging ability of DMC is very weak (Zhang et al, 2008).…”

Section: Figure 5 Effect Of Dmc On Inos Protein Expressionsupporting

confidence: 93%

“…As DMC can down-regulate iNOS/NO, our results are consistent with the previous reports on the inhibition of iNOS at mRNA and protein levels by DMC in LPS-activated rat primary microglial cells (Zhang et al, 2008) and RAW 264.7 macrophage cells (Guo et al, 2008;Kaewkroek et al, 2010). DMC may have the direct effect in inhibition of NO production via iNOS pathway as NO-scavenging ability of DMC is very weak (Zhang et al, 2008). The mechanism of action of DMC may be partly due to its potent down-regulation of intracellular ROS level and thus leading to inactivation of NF-kB and MAPKs as well as inhibiting the expression of iNOS, TNF-α, and IL-1β (Zhang et al, 2010).…”

Section: Figure 5 Effect Of Dmc On Inos Protein Expressionsupporting

confidence: 93%

“…These results are consistent with the anti-inflammatory property of DMC in inhibiting iNOS and COX-2 expression by the inhibition of NF-kB activity (Guo et al, 2008). It has been shown that all of the three curcuminoid pigments (CUR, DMC, and BDMC) significantly suppressed nitric oxide (NO) production in LPS-activated microglia and the relative potency was DMC>BDMC>CUR (Zhang et al, 2008). The absence of one methoxy group may enhance the inhibitory effect of NO production.…”

Section: Figure 5 Effect Of Dmc On Inos Protein Expressionsupporting

confidence: 78%

“…The absence of one methoxy group may enhance the inhibitory effect of NO production. The relative position of 4-phenolic hydroxy group and 3-methoxy group may be very important for the inhibitory effect of DMC (Guo et al, 2008;Zhang et al, 2008). Finally, concentrations of the inflammatory stimuli and DMC used to treat Caco-2 cells were found not to be cytotoxic.…”

Section: Figure 5 Effect Of Dmc On Inos Protein Expressionmentioning

confidence: 95%

“…DMC showed higher potency in suppressing inflammation in lipopolysaccharide (LPS)-induced NO production, iNOS, cycloxygenase-2 (COX-2) and nuclear factor-kB (NF-kB) activity in a RAW 264.7 macrophage cell line than that of bisdemethoxycurcumin (BDMC) and in vivo carrageenan-induced paw edema in mice (Guo et al, 2008). Concomitantly, DMC exhibited the strongest inhibitory activity on NO and TNF-α production in LPS-activated microglia compared to curcumin (CUR) and BDMC (Zhang et al, 2008). Preclinical and clinical research has shown mainly CUR and its anti-inflammatory properties.…”

Section: Introductionmentioning

confidence: 99%

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Demethoxycurcumin from Curcuma longa Rhizome Suppresses iNOS Induction in an in vitro Inflamed Human Intestinal Mucosa Model

Somchit

Changtam

Kimseng³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: It is known that inducible nitric oxide synthase (iNOS)/nitric oxide (NO) plays an integral role during intestinal inflammation, an important factor for colon cancer development. Natural compounds from Curcuma longa L. (Zingiberaceae) have long been a potential source of bioactive materials with various beneficial biological functions. Among them, a major active curcuminoid, demethoxycurcumin (DMC) has been shown to possess anti-inflammatory properties in lipopolysaccharide (LPS)-activated macrophages or microglia cells. However, the role of DMC on iNOS expression and NO production in an in vitro inflamed human intestinal mucosa model has not yet been elucidated. This study concerned inhibitory effects on iNOS expression and NO production of DMC in inflamed human intestinal Caco-2 cells. An in vitro model was generated and inhibitory effects on NO production of DMC at 65 μM for 24-96 h were assessed by monitoring nitrite levels. Expression of iNOS mRNA and protein was also investigated. DMC significantly decreased NO secretion by 35-41% in our inflamed cell model. Decrease in NO production by DMC was concomitant with down-regulation of iNOS at mRNA and protein levels compared to proinflammatory cytokine cocktail and LPS-treated controls. Mechanism of action of DMC may be partly due to its potent inhibition of the iNOS pathway. Our findings suggest that DMC may have potential as a therapeutic agent against inflammation-related diseases, especially in the gut.

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Section: Figure 5 Effect Of Dmc On Inos Protein Expressionsupporting

confidence: 93%

Section: Figure 5 Effect Of Dmc On Inos Protein Expressionsupporting

confidence: 93%

Section: Figure 5 Effect Of Dmc On Inos Protein Expressionsupporting

confidence: 78%

Section: Figure 5 Effect Of Dmc On Inos Protein Expressionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Demethoxycurcumin from Curcuma longa Rhizome Suppresses iNOS Induction in an in vitro Inflamed Human Intestinal Mucosa Model

Somchit

Changtam

Kimseng³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Potential Use of Curcuminoids for the Treatment of Alzheimer's Disease

Ahmed

Javed

Tariq

et al. 2017

Neuroprotective Effects of Phytochemicals in Neurological Disorders

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Demethoxycurcumin: A naturally occurring curcumin analogue with antitumor properties

Hatamipour

Ramezani

Tabassi

et al. 2018

Journal Cellular Physiology

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The eradication of cancer in a patient remains an elusive challenge despite advances in early detection and diagnosis, chemo- and immunotherapy, pinpoint radiation treatments, and expert surgical intervention. Although significant gains have been made in our understanding of cancer cell biology, a definite cure for most cancers does not exist at present. Thus, it is not surprising that the research and medical communities continue to explore the importance and therapeutic potential of natural products in their multimodality cancer treatment approach. Curcuminoids found in turmeric are one such class of natural products that have been extensively investigated for their potential to halt the progression of cancer cell proliferation and, more important, to stop metastasis from occurring. In this review, we examine one curcuminoid (demethoxycurcumin [DMC]) largely because of its increased stability and better aqueous solubility at physiological pH, unlike the more well-known curcuminoid (curcumin), which is largely unabsorbed after oral ingestion. The present review will focus on the signaling pathways that DMC utilizes to modulate the growth, invasion, and metastasis of cancer cells in an effort to provide enhanced mechanistic insight into DMC's action as it pertains to brain, ovarian, breast, lung, skin, and prostate cancer. Additionally, this review will attempt to provide an overview of DMC's mechanism of action by modulating apoptosis, cell cycle, angiogenesis, metastasis, and chemosensitivity. Lastly, it is hoped that increased understanding will be gained concerning DMC's interactive role with microRNA-551a, 5' adenosine monophosphate-activated protein kinase, nuclear factor-κB, Wnt inhibitory factor-1, and heat shock protein 70 to affect the progression of cancer.

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Comparison of inhibitory potency of three different curcuminoid pigments on nitric oxide and tumor necrosis factor production of rat primary microglia induced by lipopolysaccharide

Cited by 60 publications

References 30 publications

Demethoxycurcumin from Curcuma longa Rhizome Suppresses iNOS Induction in an in vitro Inflamed Human Intestinal Mucosa Model

Demethoxycurcumin from Curcuma longa Rhizome Suppresses iNOS Induction in an in vitro Inflamed Human Intestinal Mucosa Model

Potential Use of Curcuminoids for the Treatment of Alzheimer's Disease

Demethoxycurcumin: A naturally occurring curcumin analogue with antitumor properties

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