Abstract. Ruminant urine nitrogen (N) concentration and volume are important parameters influencing the size and N loading rate of urine patches deposited to soil. Such parameters can influence N cycling and emissions of the greenhouse gas, nitrous oxide (N 2 O) from grazed grassland, yet, there is limited information on the effect of these parameters within typical ranges reported for sheep. We used an automated, high-frequency gas monitoring system to investigate N 2 O emissions from varying urine N application rates and patch sizes under field conditions. Using artificial sheep urine, we manipulated urine N concentration to provide two urine N application rates (4 and 16 g N/L; equivalent to 200 and 800 kg N/ha). We investigated the effect of urine patch size with equal N application rates (4 · 125 cm 2 vs 500 cm 2 , at 200 and 800 kg N/ha) and the effect of patch size with unequal N application rates, but the same total amount of N applied (62.5 mL over 125 cm 2 at 800 kg N/ha and 250 mL over 500 cm 2 at 200 kg N/ha). Cumulative emissions of N 2 O generally increased with N loading rate, whether applied as one large urine patch or four smaller ones. Cumulative N 2 O emissions increased when the N was applied in four smaller urine patches compared with one large patch; this difference was significant at 800 kg N/ha, but not at 200 kg N/ha. When the total amount of N applied was held constant (1 g of N), the amount of N 2 O released was similar when urine was applied as a high N concentration small patch (800 kg N/ha) compared with a low N concentration large patch (200 kg N/ha). Urine N 2 O emission factors in this study were, on average, 10 times lower than the IPCC default of 1% for sheep excreta. This research clearly demonstrates that the chemical and physical nature of the urine patch influences N 2 O emissions, yet further research is required to gather more data on typical sheep urine volumes (individual and daily), urination frequency, urine N concentrations and the typical volumes of soil influenced by urine deposition, to provide more accurate estimates of emissions from sheep grazed pastures.