Comparison of Intraocular Treatment of DMTU and SOD Following Retinal Ischemia in Rats

Rios, Laurent; Cluzel, Jacques; Vennat, Jean-Claude; Menerath, J M; Doly, M.

doi:10.1089/jop.1999.15.547

Cited by 14 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies show that oxidative stress is a key player in retina neuronal injury in models of I/R [9–11]. To test whether WD-Hpt could reduce oxidative stress in I/R retina, we assessed formation of the peroxinitrite biomarker nitrotyrosine at 6 h after I/R by western blot analysis.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury

et al. 2015

View full text Add to dashboard Cite

Purpose To determine whether water-dispersible hesperetin (WD-Hpt) can prevent degeneration of ganglion cell neurons in the ischemic retina. Methods Ischemia reperfusion (I/R) injury was induced by increasing the intraocular pressure of mice to 110 mmHg for 40 min. Mice received daily intraperitoneal injections with either normal saline (NS, 0.3 ml/day) or WD-Hpt (0.3 ml, 200 mg/kg/day). Reactive oxygen species (ROS) was assessed by dihydroethidium and nitrotyrosine formation. Inflammation was estimated by microglial morphology in the retina. Lipopolysaccharide (LPS)-stimulated BV-2 cells were used to explore the anti-inflammatory effect of WD-Hpt on activated microglia by quantifying the expression of IL-1β using real-time quantitative reverse transcription-polymerase chain reaction. Ganglion cell loss was assessed by immunohistochemistry of NeuN. Glial activation was quantified with glial fibrillary acidic protein (GFAP) immunoreactivity. Apoptosis was evaluated with a terminal deoxynucleotidyl transferase (TUNEL) assay and immunohistochemistry of cleaved caspase-3. Phosphorylation of extracellular signal-regulated kinase (p-ERK) was surveyed by western blotting. Results WD-Hpt decreased I/R-induced ROS formation. WD-Hpt alleviated microglial activation induced by I/R and reduced mRNA levels of IL-1β in LPS-stimulated BV-2. I/R resulted in a 37 % reduction in the number of ganglion cells in the NS-treated mice, whereas the reduction was only 5 % in the WD-Hpt-treated mice. In addition, WD-Hpt mitigated the immunoreactivity of GFAP, increased expression of cleaved caspase-3, increased number of TUNEL positive cells and p-ERK after I/R. Conclusions WD-Hpt protected ganglion cells from I/R injury by inhibiting oxidative stress and modulating cell death signaling. Moreover, WD-Hpt had an anti-inflammatory effect through the suppression of activated microglia.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Oxidative stress is critically involved in neuronal cell death in ischemic retinopathy [9–11]. It causes diverse pathways such as inflammation [12, 13], proliferation [14] and apoptosis [15].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury

et al. 2015

View full text Add to dashboard Cite

show abstract

“…[31][32][33] NADPH oxidase is a major source of ROS in vascular cells. To understand the potential role of NADPH oxidase in neuronal injury in retinal I/R, the expression of different NOX isoforms and p47 phox and p22 phox subunits was analyzed at 6 hours after retinal ischemic injury.…”

Section: Elevation Of Nox2 Nadph Oxidase Expression and Activity Durimentioning

confidence: 99%

Neuroprotection from Retinal Ischemia/Reperfusion Injury by NOX2 NADPH Oxidase Deletion

Yokota

Narayanan

Zhang

et al. 2011

Invest. Ophthalmol. Vis. Sci.

106

View full text Add to dashboard Cite

show abstract

“…Injury mediated by free radicals is a major cause of damage in ischaemic tissue after reperfusion (McCord, 1985), but it can be reduced by the use of antioxidants (Martz et al, 1989;Punch et al, 1991;Ambrosio & Flaherty, 1992;Karwatowska-Prokopczuk et al, 1992;Ernster, 1998;Rios et al, 1999). This is the first report of free oxygen radicals being generated at the time of oocyte aspiration from ovaries that have been stored for a long time (several hours), and these chemicals impair the developmental competence of oocytes.…”

Section: Discussionmentioning

confidence: 99%

Free oxygen radicals are generated at the time of aspiration of oocytes from ovaries that have been stored for a long time

Iwata¹,

Ohota²,

Hashimoto³

et al. 2003

Zygote

View full text Add to dashboard Cite

In ischaemic tissues, reperfusion induces acute injury and functional changes. In this work, ovaries were stored for various times, and superoxide dismutase (SOD) and dimethylthiourea (DTMU) were used at the time of oocyte aspiration. We then attempted to determine whether free oxygen radicals are generated at oocyte aspiration and whether they impair the developmental competence of oocytes. Over 2 mM of DMTU and 1000 U/ml of SOD significantly improved the rate of blastulation 8 days after insemination. For ovaries that were preserved for 3 and 7 h, using antioxidants also significantly improved the rate of blastulation 8 days after insemination. However, no effect was observed on oocytes from ovaries preserved for 1 h. We examined how the antioxidants affected the presence of germinal vesicles, chromatin configuration, and polar body extrusion 6 or 21 h after culture. Chromatin configuration was classified into three groups according to the amount of chromatin condensation (group 1, strong condensation; group 2, moderate; group 3, slight). Storing ovaries for a long time decreased the frequency of occurrence of group 2, but increased groups 1 and 3. However, using antioxidants at oocyte aspiration decreased the frequency of group 3 and increased group 1. Moreover, there was no difference in the rate of germinal vesicle breakdown and polar body extrusion. Our results show that preserving ovaries for a long time induces the generation of free oxygen radicals and that these chemicals impair oocyte viability. Using antioxidants at oocyte aspiration was beneficial for embryo production.

show abstract

Comparison of Intraocular Treatment of DMTU and SOD Following Retinal Ischemia in Rats

Cited by 14 publications

References 15 publications

Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury

Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury

Neuroprotection from Retinal Ischemia/Reperfusion Injury by NOX2 NADPH Oxidase Deletion

Free oxygen radicals are generated at the time of aspiration of oocytes from ovaries that have been stored for a long time

Contact Info

Product

Resources

About